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Evaluation of the biological and wound healing activities of Centaurea virgata

Year 2020, Volume: 10 Issue: 3, 304 - 308, 29.09.2020

Halil Aksoy

https://doi.org/10.33808/marusbed.789734
Cited By: 2

Abstract

Objective: Plants have significant potential effects in treating the wound. The vast majority of plants are used by the public in the treatment of wounds. The aim of this study was to investigate the antioxidant and in vitro wound healing activities of Centaurea virgata (C. Virgata).
Methods: The antioxidant activities of extracts were examined by 2,2-diphenyl-1-picryl-hydrazyl (DPPH) and ABTS methods. The amount of total phenolic was determined using the Folin-Ciocalteu reagent (FCR) method. In addition, the antiproliferative activity of different extracts on fibroblast cells were performed by MTT method and in vitro wound healing activity of ethanol extract of C. Virgata was determined by scratch assay.
Results: Ethyl acetate extract of C. virgata (CVEA) had the highest DPPH radical scavenging activity with a IC50 value of 138.7 μg/mL. On the other hand, hexane (CVH) and aqueous ethanol (CVAE) revealed a very poor antioxidant activity with a IC50 values of 824,8 µg/mL and 610,3 ± 12,8 µg/mL, respectively. According to ABTS results, it was determined that the CVEA extract had stronger radical scavenging activity than the other extracts. On the other hand, among all of the extracts analysed, it was found that phenolic contents of CVEA extract had higher than other extracts. Except for CVAE extract, other extracts showed antiproliferative activity depending on the dose.
Conclusion: According to the obtained results, our finding suggests that CVAE extract has a migratory effect on fibroblasts and that CVAE might be a potential therapeutic agent for wound healing.

Keywords

Centaurea virgata antioxidant activity wound healing

Thanks

The author would like to thank Dr. İsmail Şenkardeş for his help in identification of the plant material.

References

  • [1] Aksoy H, Özakpınar OB. Yara İyileşmesi ve Oksidatif Stres. Marmara Pharmaceutical Journal 2014;18:153-158.
  • [2] Aksoy H, Apikoğlu-Rabus S, Uras F, Savcı N, F. Alatlı C, Erseven G, Emekli N. Evaluation of the Effect of Fibrin Glue Prepared from Single-Donor Plasma on Wound Healing in Rats. Hacettepe University Journal of the Faculty of Pharmacy 2009; 29(2): 83-93.
  • [3] Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res. 2010, 89(3):219-29.
  • [4] Schreier T, Degen E, Baschong W. Fibroblast migration and proliferation during in vitro wound healing. A quantitative comparison between various growth factors and a low molecular weight blood dialysate used in the clinic to normalize impaired wound healing. Res Exp Med (Berl). 1993;193(4):195-205.
  • [5] Aksoy H, Sen A, Sancar M, Sekerler T, Akakin D, Bitis L, Uras F, Kultur S, Izzettin FV. Ethanol extract of Cotinus coggygria leaves accelerates wound healing process in diabetic rats. Pharm Biol 2016; 54(11): 2732-2736.
  • [6] Aslam MS, Ahmad MS, Mamat AS, Ahmad MZ, Salam F. Antioxidant and Wound Healing Activity of Polyherbal Fractions of Clinacanthus nutans and Elephantopus scaber. Evid Based Complement Alternat Med. 2016; 4685246.
  • [7] Chin CY, Jalil J, Ng PY, Ng SF. Development and formulation of Moringa oleifera standardised leaf extract film dressing for wound healing application. J Ethnopharmacol 2018, 15;212:188-199.
  • [8] Aksoy H, Demirbağ Ç, Şen A, Şekerler T, Özakpınar Ö, Şener A, Ahmad S, Tetik S. Evaluation of biochemical parameters in Rubus tereticaulis treated rats and its implications in wound healing. Mol Cell Biochem. 2020;c472(1-2):67-78.
  • [9] Garcia-Jacas N, Susanna A, Mozaffarian V, Ilarslan R. The natural delimitation of Centaurea (Asteraceae: Cardueae): ITS sequence analysis of the Centaurea jacea group. Plant Systematics and Evol 2000; 223 (3): 185-199.
  • [10] Köse YB, İşcan G, Demirci B, Başer KHC, Çelik S. Antimicrobial activity of the essential oil of Centaurea aladagensis. Fitoterapia 200778 (3): 253-254.
  • [11] Ozsoy N, Kultur S, Yilmaz-Ozden T, Celik BO, Can A, Melikoglu G. Antioxidant, AntiInflammatory, Acetylcholinesterase Inhibitory and Antimicrobial Activities of Turkish Endemic Centaurea antiochia var-Praealta. J Food Biochem 2015;39 (6): 771-776.
  • [12] Yirtici U, Goger F, Sarimahmut M, Ergene A. Cytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell line. Turkish Journal of Biology 2017;41(2): 370-377.
  • [13] Zou Y, Chang SK, Gu Y, Qian SY. Antioxidant activity and phenolic compositions of lentil (Lens culinaris var. morton) extract and its fractions. J Agric Food Chem. 2011; 59(6): 2268-2276.
  • [14] Ozsoy N, Can A, Yanardağ R, Akev N. Antioxidant activity of Sm ilax excelsa L. leaf extracts. Food Chem 2008; 110(3): 571¬ 583. [15] Raina R, Parwez S, Verma PK, Pankaj NK. Medicinal plants and their role in wound healing. Vet Scan 2008; 3(1): 21-26.
  • [16] Kumarasamyraja D, Jeganathan NS, Manavalan R. A review on medicinal plants with potential wound healing activity. International Journal of Pharma Sciences 2012; 2(4): 105-111.
  • [17] Syarina PN, Karthivashan G, Abas F, Arulselvan P, Fakurazi S. Wound healing potential of Spirulina platensis extracts on human dermal fibroblast cells. EXCLI J. 2015, 2;14:385-93.
  • [18] Pitz Hda S, Pereira A, Blasius MB, Voytena AP, Affonso RC, Fanan S, Trevisan AC, Ribeiro-do-Valle RM, Maraschin M. In Vitro Evaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract. Oxid Med Cell Longev. 2016; 2016:3403586.
  • [19] Keser S, Keser F, Türkoğlu İ, Kaygılı Ö, Tekin S, Demir E, Karatepe M, Yılmaz Ö, Kırbağ S, Sandal S, Türkoğlu S 2020. In Vitro Biological Evaluation and Phytochemical Contents of Three Centaurea L. Species Growing from Eastern Anatolia in Turkey. KSU J. Agric Nat 23 (1): 148-156.
  • [20] Goto T, Hagiwara K, Shirai N, Yoshida K, Hagiwara H. Apigenin inhibits osteoblastogenesis and osteoclastogenesis and prevents bone loss in ovariectomized mice. Cytotechnology. 2015;67(2):357-65.
  • [21] Csapi B, Hajdú Z, Zupkó I, Berényi A, Forgo P, Szabó P, Hohmann J. Bioactivity-guided isolation of antiproliferative compounds from Centaurea arenaria. Phytother Res. 2010; 24(11):1664-1669.
  • [22] Androutsopoulos V, Arroo RR, Hall JF, Surichan S, Potter GA. Antiproliferative and cytostatic effects of the natural product eupatorin on MDA-MB-468 human breast cancer cells due to CYP1-mediated metabolism. Breast Cancer Res. 2008;10(3):R39.
  • [23] Tuzun BS, Hajdu Z, Orban-Gyapai O, Zomborszki ZP, Jedlinszki N, Forgo P, Kıvcak B, Hohmann J. Isolation of Chemical Constituents of Centaurea virgata Lam. and Xanthine Oxidase Inhibitory Activity of the Plant Extract and Compounds. Med Chem. 2017;13(5):498-502.
  • [24] Miliauskas G, Venskutonis PR, van Beek BA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry 2004; 85: 231-237.
  • [25] Demirci S, Özhatay N. An Ethnobotanical Study in Kahramanmaraş (Turkey); Wild Plants Used For Medicinal Purpose in Andırın, Kahramanmaraş, Turk J Pharm Sci. 2012; 9 (1): 75-92.

Year 2020, Volume: 10 Issue: 3, 304 - 308, 29.09.2020

Halil Aksoy

https://doi.org/10.33808/marusbed.789734
Cited By: 2

Abstract

References

  • [1] Aksoy H, Özakpınar OB. Yara İyileşmesi ve Oksidatif Stres. Marmara Pharmaceutical Journal 2014;18:153-158.
  • [2] Aksoy H, Apikoğlu-Rabus S, Uras F, Savcı N, F. Alatlı C, Erseven G, Emekli N. Evaluation of the Effect of Fibrin Glue Prepared from Single-Donor Plasma on Wound Healing in Rats. Hacettepe University Journal of the Faculty of Pharmacy 2009; 29(2): 83-93.
  • [3] Guo S, Dipietro LA. Factors affecting wound healing. J Dent Res. 2010, 89(3):219-29.
  • [4] Schreier T, Degen E, Baschong W. Fibroblast migration and proliferation during in vitro wound healing. A quantitative comparison between various growth factors and a low molecular weight blood dialysate used in the clinic to normalize impaired wound healing. Res Exp Med (Berl). 1993;193(4):195-205.
  • [5] Aksoy H, Sen A, Sancar M, Sekerler T, Akakin D, Bitis L, Uras F, Kultur S, Izzettin FV. Ethanol extract of Cotinus coggygria leaves accelerates wound healing process in diabetic rats. Pharm Biol 2016; 54(11): 2732-2736.
  • [6] Aslam MS, Ahmad MS, Mamat AS, Ahmad MZ, Salam F. Antioxidant and Wound Healing Activity of Polyherbal Fractions of Clinacanthus nutans and Elephantopus scaber. Evid Based Complement Alternat Med. 2016; 4685246.
  • [7] Chin CY, Jalil J, Ng PY, Ng SF. Development and formulation of Moringa oleifera standardised leaf extract film dressing for wound healing application. J Ethnopharmacol 2018, 15;212:188-199.
  • [8] Aksoy H, Demirbağ Ç, Şen A, Şekerler T, Özakpınar Ö, Şener A, Ahmad S, Tetik S. Evaluation of biochemical parameters in Rubus tereticaulis treated rats and its implications in wound healing. Mol Cell Biochem. 2020;c472(1-2):67-78.
  • [9] Garcia-Jacas N, Susanna A, Mozaffarian V, Ilarslan R. The natural delimitation of Centaurea (Asteraceae: Cardueae): ITS sequence analysis of the Centaurea jacea group. Plant Systematics and Evol 2000; 223 (3): 185-199.
  • [10] Köse YB, İşcan G, Demirci B, Başer KHC, Çelik S. Antimicrobial activity of the essential oil of Centaurea aladagensis. Fitoterapia 200778 (3): 253-254.
  • [11] Ozsoy N, Kultur S, Yilmaz-Ozden T, Celik BO, Can A, Melikoglu G. Antioxidant, AntiInflammatory, Acetylcholinesterase Inhibitory and Antimicrobial Activities of Turkish Endemic Centaurea antiochia var-Praealta. J Food Biochem 2015;39 (6): 771-776.
  • [12] Yirtici U, Goger F, Sarimahmut M, Ergene A. Cytotoxic and apoptotic effects of endemic Centaurea fenzlii Reichardt on the MCF-7 breast cancer cell line. Turkish Journal of Biology 2017;41(2): 370-377.
  • [13] Zou Y, Chang SK, Gu Y, Qian SY. Antioxidant activity and phenolic compositions of lentil (Lens culinaris var. morton) extract and its fractions. J Agric Food Chem. 2011; 59(6): 2268-2276.
  • [14] Ozsoy N, Can A, Yanardağ R, Akev N. Antioxidant activity of Sm ilax excelsa L. leaf extracts. Food Chem 2008; 110(3): 571¬ 583. [15] Raina R, Parwez S, Verma PK, Pankaj NK. Medicinal plants and their role in wound healing. Vet Scan 2008; 3(1): 21-26.
  • [16] Kumarasamyraja D, Jeganathan NS, Manavalan R. A review on medicinal plants with potential wound healing activity. International Journal of Pharma Sciences 2012; 2(4): 105-111.
  • [17] Syarina PN, Karthivashan G, Abas F, Arulselvan P, Fakurazi S. Wound healing potential of Spirulina platensis extracts on human dermal fibroblast cells. EXCLI J. 2015, 2;14:385-93.
  • [18] Pitz Hda S, Pereira A, Blasius MB, Voytena AP, Affonso RC, Fanan S, Trevisan AC, Ribeiro-do-Valle RM, Maraschin M. In Vitro Evaluation of the Antioxidant Activity and Wound Healing Properties of Jaboticaba (Plinia peruviana) Fruit Peel Hydroalcoholic Extract. Oxid Med Cell Longev. 2016; 2016:3403586.
  • [19] Keser S, Keser F, Türkoğlu İ, Kaygılı Ö, Tekin S, Demir E, Karatepe M, Yılmaz Ö, Kırbağ S, Sandal S, Türkoğlu S 2020. In Vitro Biological Evaluation and Phytochemical Contents of Three Centaurea L. Species Growing from Eastern Anatolia in Turkey. KSU J. Agric Nat 23 (1): 148-156.
  • [20] Goto T, Hagiwara K, Shirai N, Yoshida K, Hagiwara H. Apigenin inhibits osteoblastogenesis and osteoclastogenesis and prevents bone loss in ovariectomized mice. Cytotechnology. 2015;67(2):357-65.
  • [21] Csapi B, Hajdú Z, Zupkó I, Berényi A, Forgo P, Szabó P, Hohmann J. Bioactivity-guided isolation of antiproliferative compounds from Centaurea arenaria. Phytother Res. 2010; 24(11):1664-1669.
  • [22] Androutsopoulos V, Arroo RR, Hall JF, Surichan S, Potter GA. Antiproliferative and cytostatic effects of the natural product eupatorin on MDA-MB-468 human breast cancer cells due to CYP1-mediated metabolism. Breast Cancer Res. 2008;10(3):R39.
  • [23] Tuzun BS, Hajdu Z, Orban-Gyapai O, Zomborszki ZP, Jedlinszki N, Forgo P, Kıvcak B, Hohmann J. Isolation of Chemical Constituents of Centaurea virgata Lam. and Xanthine Oxidase Inhibitory Activity of the Plant Extract and Compounds. Med Chem. 2017;13(5):498-502.
  • [24] Miliauskas G, Venskutonis PR, van Beek BA. Screening of radical scavenging activity of some medicinal and aromatic plant extracts. Food Chemistry 2004; 85: 231-237.
  • [25] Demirci S, Özhatay N. An Ethnobotanical Study in Kahramanmaraş (Turkey); Wild Plants Used For Medicinal Purpose in Andırın, Kahramanmaraş, Turk J Pharm Sci. 2012; 9 (1): 75-92.
There are 24 citations in total.

Details

Primary Language English
Subjects Health Care Administration
Journal Section Articles
Authors

Halil Aksoy 0000-0002-7256-0037

Publication Date September 29, 2020
Submission Date September 2, 2020
Published in Issue Year 2020 Volume: 10 Issue: 3

Cite

APA Aksoy, H. (2020). Evaluation of the biological and wound healing activities of Centaurea virgata. Clinical and Experimental Health Sciences, 10(3), 304-308. https://doi.org/10.33808/marusbed.789734
AMA Aksoy H. Evaluation of the biological and wound healing activities of Centaurea virgata. Clinical and Experimental Health Sciences. September 2020;10(3):304-308. doi:10.33808/marusbed.789734
Chicago Aksoy, Halil. “Evaluation of the Biological and Wound Healing Activities of Centaurea Virgata”. Clinical and Experimental Health Sciences 10, no. 3 (September 2020): 304-8. https://doi.org/10.33808/marusbed.789734.
EndNote Aksoy H (September 1, 2020) Evaluation of the biological and wound healing activities of Centaurea virgata. Clinical and Experimental Health Sciences 10 3 304–308.
IEEE H. Aksoy, “Evaluation of the biological and wound healing activities of Centaurea virgata”, Clinical and Experimental Health Sciences, vol. 10, no. 3, pp. 304–308, 2020, doi: 10.33808/marusbed.789734.
ISNAD Aksoy, Halil. “Evaluation of the Biological and Wound Healing Activities of Centaurea Virgata”. Clinical and Experimental Health Sciences 10/3 (September 2020), 304-308. https://doi.org/10.33808/marusbed.789734.
JAMA Aksoy H. Evaluation of the biological and wound healing activities of Centaurea virgata. Clinical and Experimental Health Sciences. 2020;10:304–308.
MLA Aksoy, Halil. “Evaluation of the Biological and Wound Healing Activities of Centaurea Virgata”. Clinical and Experimental Health Sciences, vol. 10, no. 3, 2020, pp. 304-8, doi:10.33808/marusbed.789734.
Vancouver Aksoy H. Evaluation of the biological and wound healing activities of Centaurea virgata. Clinical and Experimental Health Sciences. 2020;10(3):304-8.

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