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Yüksek yağlı diyet ile beslenen ve tam kalınlıkta deri defekti modeli oluşturulan sıçanlarda laktoferrinin etkisi

Year 2023, Volume: 48 Issue: 4, 1396 - 1404, 29.12.2023
https://doi.org/10.17826/cumj.1396328

Abstract

Amaç: Laktoferrin, geniş bir biyoaktivite yelpazesine sahip süt proteini ailesinin bir üyesidir ve birçok mikrop, virüs ve diğer patojenlere karşı tedavi edici etkilere sahiptir. Bu çalışmanın amacı laktoferinin, obeziteye bağlı yara iyileşmesine karşı iyileştirici etkisi göstermesi amaçlanmaktadır.
Gereç ve Yöntem: Bu amaçla 48 adet erkek sıçan Kontrol (C) (n=8), Tam kalınlıkta deri defekti modeli (FT) (n=8), Sığır Laktoferrin (bLf) (n= 8), Yüksek Yağlı Diyet olarak gruplandırıldı. (HFD) + Sığır Laktoferrin (bLf) (n = 8)(200 mg/kg), Yüksek Yağlı Diyet (HFD)+Tam kalınlıkta cilt defekti modeli (FT) (n=8) ve HFD+Tam kalınlıkta cilt defekti modeli (FT) + Sığır Laktoferrin bLf (n=8). Yüksek Yağlı Diyet + bLf ve Yüksek Yağlı Diyet + Tam Kalınlık cilt defekti + bLf grubundaki sıçanlara yüksek yağlı diyet ve sığır laktoferrin oral olarak verildi.IL-6 ve VEGF biyokimyasal parametrelerine serumda bakılmıştır.
Bulgular: Grupların serum IL-6 protein miktarları incelendiğinde 21. günde en yüksek HFD+FT+bLf grubunda, diğer grup olan serum VEGF protein miktarları ise FT grubunda en düşüktü. Biyokimyasal parametreler değerlendirildiğinde bu miktarların 21. günde en yüksek HFD+FT+bLf grubunda, en düşük ise FT grubunda olduğu görüldü.
Sonuç: Laktoferrinin yara iyileşmesini hızlandırdığı gösterilmiştir ve laktoferrinin kolaylıkla bulunabilmesi ve sütte bol miktarda bulunması, onu gelecekte yara iyileşmesi ve iltihaplanma için heyecan verici bir tedavi seçeneği haline getirmektedir.

Supporting Institution

Gaziantep Üniversitesi Bilimsel araştırma projeleri birimi (Protokol no: TF. YLT.21.31).

References

  • Chooi YC, Ding C, Magkos F. The epidemiology of obesity. Metabolism. 2019;92:6-10.
  • Sarma S, Sockalingam S, Dash S. Obesity as a multisystem disease: Trends in obesity rates and obesity‐related complications. Diabetes Obes Metab. 2021;23:3-16.
  • Kato Y, Iwata T, Morikawa S, Yamato M, Okano T, Uchigata Y. Allogeneic transplantation of an adipose-derived stem cell sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity. Diabetes. 2015;64:2723-34.
  • Lee RC, Leem H, Lee J, Park KC. Reversal of silver sulfadiazine-impaired wound healing by epidermal growth factor. Biomaterials. 2005;26:4670-6.
  • González-Chávez SA, Arévalo-Gallegos S, Rascón-Cruz Q. Lactoferrin: structure, function and applications. Int J Antimicrob Agents. 2009;33:301.e1-8.
  • Conneely OM. Antiinflammatory activities of lactoferrin. J Am Coll Nutr. 2001;20:389-95.
  • Farnaud S, Evans RW. Lactoferrin a multifunctional protein with antimicrobial properties. Mol Immunol. 2003;40:395-405.
  • Berkhout B, Floris R, Recio I, Visser S. Antibacterial effects of the milk protein lactoferrin. Agro Food Industry Hi Tech. 2003;14:32-3.
  • Chen Y, Liu Q, Guo D. Emerging coronaviruses: genome structure, replication, and pathogenesis. J Med Virol. 2020;92:418-23.
  • Hessin A, Hegazy R, Hassan A, Yassin N, Kenawy S. Lactoferrin enhanced apoptosis and protected against thioacetamide-induced liver fibrosis in rats. Open Access Maced J Medical Sci. 2015;3:195-201.
  • Reeves PG, Nielsen FH, Fahey Jr GC. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993;123:1939-51.
  • Nozari S, Fathi Maroufi N, Nouri M, Paytakhti Oskouei M, Shiralizade J, Yekani F et al. Decreasing serum homocysteine and hypocholesterolemic effects of Bovine lactoferrin in male rat fed with high-cholesterol diet. J Cardiovasc Thorac Res. 2018;10:203-8.
  • Kruzel ML, Bacsi A, Choudhury B, Sur S, Boldogh I. Lactoferrin decreases pollen antigen‐induced allergic airway inflammation in a murine model of asthma. Immunology. 2006;119:159-66.
  • Bastard JP, Jardel C, Bruckert E, Blondy P, Capeau J, Laville M et al. Elevated levels of interleukin 6 are reduced in serum and subcutaneous adipose tissue of obese women after weight loss. J Clin Endocrinol Metab. 2000;85:3338-42.
  • Andrich DE, Melbouci L, Ou Y, Auclair N, Mercier J, Grenier JC et al. A short-term high-fat diet alters glutathione levels and IL-6 gene expression in oxidative skeletal muscles of young rats. Front Physiol. 2019;10:372.
  • Elmenam HS, Farouk MH. Bovine lactoferrin in preterm labor with sterile inflammation. The Scientific Journal of Al-Azhar Medical Faculty Girls. 2021;5:299-306.
  • Orhan C, Akdemir F, Tuzcu M, Sahin N, Yilmaz I, Deshpande J et al. Mesozeaxanthin protects retina from oxidative stress in a rat model. J Ocul Pharmacol. Ther. 2016;631-7.
  • Choi J, Kim J, Kim J, Jeong W, Jo T, Lee HW et al. A high-fat diet in the absence of obesity increases lymphangiogenesis by inducing VEGF-C in a murine lymphedema model. Plast Reconstr Surg. 2023;152:87-96.
  • Saaristo A, Tammela T, Farkkilā A, Kärkkäinen M, Suominen E, Yla-Herttuala S et al. Vascular endothelial growth factor-C accelerates diabetic wound healing. Am J Pathol. 2006;169:1080-7.
  • Shams F, Moravvej H, Hosseinzadeh S, Mostafavi E, Bayat H, Kazemi B et al. Overexpression of VEGF in dermal fibroblast cells accelerates the angiogenesis and wound healing function: in vitro and in vivo studies. Sci Rep. 2022;12:18529.

Effect of lactoferrin in rats fed with a high-fat diet and with a full-thickness skin defect model

Year 2023, Volume: 48 Issue: 4, 1396 - 1404, 29.12.2023
https://doi.org/10.17826/cumj.1396328

Abstract

Purpose: Lactoferrin is a member of the milk protein family with a broad spectrum of bioactivities and has therapeutic effects against many microbes, viruses, and other pathogens. This study aims to show the healing effect of lactoferrin against obesity-related wound healing.
Materials and Methods: In this study, 48 male rats were grouped as the Control (C) (n =8), Full-thickness skin defect model (FT) (n =8), Bovine Lactoferrin (bLf) (200 mg/kg)(n= 8), High Fat Diet (HFD) + Bovine Lactoferrin (bLf) (n = 8), High-Fat Diet (HFD)+Full-thickness skin defect model (FT) (n=8), and HFD+Full-thickness skin defect model (FT) + Bovine Lactoferrin bLf (n=8). High-Fat Diet + bLf and High-Fat Diet + Full-thickness skin defect + bLf group rats were given a high-fat diet and Bovine Lactoferrin orally. IL-6 and VEGF biochemical parameters were examined in serum.
Results: When the serum IL-6 protein amounts of the groups were examined, it was the highest in the HFD+FT+bLf group on the 21st day, and the lowest in the FT group, when the serum VEGF protein amounts, which were the other biochemical parameters, were evaluated, these amounts were found to be the highest in the HFD+FT+bLf group and the lowest in the FT group on the 21st day.
Conclusion: Lactoferrin has been shown to accelerate wound healing, and the fact that lactoferrin is readily available and abundant in milk makes it an exciting treatment option for wound healing and inflammation in the future.

Supporting Institution

Gaziantep University Research Project Management Unit for funding. (Grant: TF. YLT.21.31).

References

  • Chooi YC, Ding C, Magkos F. The epidemiology of obesity. Metabolism. 2019;92:6-10.
  • Sarma S, Sockalingam S, Dash S. Obesity as a multisystem disease: Trends in obesity rates and obesity‐related complications. Diabetes Obes Metab. 2021;23:3-16.
  • Kato Y, Iwata T, Morikawa S, Yamato M, Okano T, Uchigata Y. Allogeneic transplantation of an adipose-derived stem cell sheet combined with artificial skin accelerates wound healing in a rat wound model of type 2 diabetes and obesity. Diabetes. 2015;64:2723-34.
  • Lee RC, Leem H, Lee J, Park KC. Reversal of silver sulfadiazine-impaired wound healing by epidermal growth factor. Biomaterials. 2005;26:4670-6.
  • González-Chávez SA, Arévalo-Gallegos S, Rascón-Cruz Q. Lactoferrin: structure, function and applications. Int J Antimicrob Agents. 2009;33:301.e1-8.
  • Conneely OM. Antiinflammatory activities of lactoferrin. J Am Coll Nutr. 2001;20:389-95.
  • Farnaud S, Evans RW. Lactoferrin a multifunctional protein with antimicrobial properties. Mol Immunol. 2003;40:395-405.
  • Berkhout B, Floris R, Recio I, Visser S. Antibacterial effects of the milk protein lactoferrin. Agro Food Industry Hi Tech. 2003;14:32-3.
  • Chen Y, Liu Q, Guo D. Emerging coronaviruses: genome structure, replication, and pathogenesis. J Med Virol. 2020;92:418-23.
  • Hessin A, Hegazy R, Hassan A, Yassin N, Kenawy S. Lactoferrin enhanced apoptosis and protected against thioacetamide-induced liver fibrosis in rats. Open Access Maced J Medical Sci. 2015;3:195-201.
  • Reeves PG, Nielsen FH, Fahey Jr GC. AIN-93 purified diets for laboratory rodents: final report of the American Institute of Nutrition ad hoc writing committee on the reformulation of the AIN-76A rodent diet. J Nutr. 1993;123:1939-51.
  • Nozari S, Fathi Maroufi N, Nouri M, Paytakhti Oskouei M, Shiralizade J, Yekani F et al. Decreasing serum homocysteine and hypocholesterolemic effects of Bovine lactoferrin in male rat fed with high-cholesterol diet. J Cardiovasc Thorac Res. 2018;10:203-8.
  • Kruzel ML, Bacsi A, Choudhury B, Sur S, Boldogh I. Lactoferrin decreases pollen antigen‐induced allergic airway inflammation in a murine model of asthma. Immunology. 2006;119:159-66.
  • Bastard JP, Jardel C, Bruckert E, Blondy P, Capeau J, Laville M et al. Elevated levels of interleukin 6 are reduced in serum and subcutaneous adipose tissue of obese women after weight loss. J Clin Endocrinol Metab. 2000;85:3338-42.
  • Andrich DE, Melbouci L, Ou Y, Auclair N, Mercier J, Grenier JC et al. A short-term high-fat diet alters glutathione levels and IL-6 gene expression in oxidative skeletal muscles of young rats. Front Physiol. 2019;10:372.
  • Elmenam HS, Farouk MH. Bovine lactoferrin in preterm labor with sterile inflammation. The Scientific Journal of Al-Azhar Medical Faculty Girls. 2021;5:299-306.
  • Orhan C, Akdemir F, Tuzcu M, Sahin N, Yilmaz I, Deshpande J et al. Mesozeaxanthin protects retina from oxidative stress in a rat model. J Ocul Pharmacol. Ther. 2016;631-7.
  • Choi J, Kim J, Kim J, Jeong W, Jo T, Lee HW et al. A high-fat diet in the absence of obesity increases lymphangiogenesis by inducing VEGF-C in a murine lymphedema model. Plast Reconstr Surg. 2023;152:87-96.
  • Saaristo A, Tammela T, Farkkilā A, Kärkkäinen M, Suominen E, Yla-Herttuala S et al. Vascular endothelial growth factor-C accelerates diabetic wound healing. Am J Pathol. 2006;169:1080-7.
  • Shams F, Moravvej H, Hosseinzadeh S, Mostafavi E, Bayat H, Kazemi B et al. Overexpression of VEGF in dermal fibroblast cells accelerates the angiogenesis and wound healing function: in vitro and in vivo studies. Sci Rep. 2022;12:18529.
There are 20 citations in total.

Details

Primary Language English
Subjects Medical Biochemistry - Proteins, Peptides and Proteomics
Journal Section Research
Authors

Gönül Sena Yıldırım 0000-0002-0512-1958

Ahmet Sarper Bozkurt 0000-0002-7293-0974

Mustafa Örkmez 0000-0001-5255-0504

Hasan Ulusal 0000-0003-3890-2088

Publication Date December 29, 2023
Submission Date November 26, 2023
Acceptance Date December 26, 2023
Published in Issue Year 2023 Volume: 48 Issue: 4

Cite

MLA Yıldırım, Gönül Sena et al. “Effect of Lactoferrin in Rats Fed With a High-Fat Diet and With a Full-Thickness Skin Defect Model”. Cukurova Medical Journal, vol. 48, no. 4, 2023, pp. 1396-04, doi:10.17826/cumj.1396328.