Abstract
Since biochemical tests play an important role in the decisions of clinicians, it become necessary for each region andeach laboratory to have different biochemical results. When the reference population is obtained from hospital data, thehealthy and the diseased groups are also mixed. By using Bhattacharya procedure, it is possible to have a healthy subgroup out of the mixed data. The main objective of this study is to introduce Bhattacharya procedure that will providea healthy selection of population while calculating the reference interval by using hospital data, and to have a practicefor Aspartat Transaminase (AST) parameter. Big data set is required in order to use Bhattacharya method (n≈8000).Aspartat Transaminase (AST) data of 40.486 registered individuals applying only once to the polyclinic of Health,Research and Application Hospital of Mersin University, Clinic Biochemical Laboratory is used in this study. Referenceinterval is also calculated by using the nonparametric method and the same data. In the nonparametric method, thereference interval is calculated in the range of [12.28-49.00] with 95% confidence. According to Bhattacharya method,it is calculated as [7.10-32.26]. The comparison of the results shows that in Bhattacharya method, the reference intervalis narrower in comparison to the nonparametric method
References
- clpmag.com [Internet].Utah Governor Huntsman recognizes medical laboratory week. Clinical Lab Products. Issue Stories. May 2006. [Updated: 2006 May; http://www.clpmag.com/issues/articles/2006- 05_03.asp. Available from:
- Shirts HB, Wilson AR, Jackson BR. Partitioning reference intervals by use of genetic information. Clinical Chemistry. 2011; 57(3): 475-81.
- Kataye A, Balciza C, Seccombe DW. Establishing reference intervals for clinical laboratory test results. Is there a better way? Clinical chemistry / estimating reference intervals. Am J Clin Pathol. 2010; 133(1): 180-6.
- Clinical and laboratory standards institute. Defining, establishing, and verifying reference intervals in the clinical laboratory; approved guideline. Third Edition. CLSI document C28-A3. Wayne, PA: Clinical and Laboratory Standards Institute; 2008.
- International federation of clinical chemistry and international committee for standardization in hematology: Approved recommendation (1986) on the theory of reference values. Part I. The concept of reference values. J Clin Chem Clin Biochem. 1987; 25(5): 337-42.
- Solberg HE, Grasberck R. Reference values. Adv Clin Chem. 1989; 27(1): 1-79.
- Nagayama I, Yamamoto K, Saito K, Kuzuya T, Saito T. Subject-based reference values in thyroid tests. Endocrine Journal. 1993; 40(5): 557-62.
- Baadenhuijsen H, Smit JC. Indirect estimation of clinical chemical reference intervals from total hospital patient data: Application of a modified bhattacharya procedure. J Clin Chem Clin Biochem. 1985; 23(12): 829-39.
- Bhattacharya CG. A simple method of resolution of a distribution into Gaussian components. Biometrics. 1967; 23(1): 115-35.
- Calleja J. Bhattacharya analysis. AACB SES; 2010; Sydney. http://oldsite.aacb.asn.au/files/File/SES/2010/AQ/1130- 1200%20Calleja.pdf. from:
- Usta M. Koagülasyon testlerinin referans aralıklarının indirekt belirlenmesinde bhattacharya ve hoffman metotlarının kalite kontrol prosedürleri olarak karşılaştırılması [Uzmanlık Tezi]. İstanbul: T.C. Sağlık Bakanlığı İstanbul Eğitim ve Araştırma Hastanesi Klinik Biyokimya Laboratuvarı; 2009.
Abstract
Biyokimyasal testlerin, klinisyenlerin verdiği kararlarda önemli rol oynaması, her bölgenin ve her laboratuvarın farklı biyokimyasal sonuçlara sahip olmasını gerektirmiştir. Referans popülasyon, hastane verilerinden elde edildiği zaman sağlıklı ve hasta grupları birbirine karışır. Bhattacharya prosedürü kullanılarak bu karışık verilerden sağlıklı alt grup elde edilebilir. Bu çalışmanın amacı, hastane verileri ile referans aralık hesaplaması yapılırken, sağlıklı popülasyonun seçimini sağlayacak Bhattacharya prosedürlerini tanıtmak ve Aspartat Transaminaz (AST) parametresi için bir uygulama yapmaktır. Bhattacharya yönteminin kullanılabilmesi için büyük veri setlerine ihtiyaç vardır (n≈8000). Çalışmada Bhattacharya yönteminin adımlarını anlatmak için Mersin Üniversitesi Sağlık Araştırma ve Uygulama Hastanesi Klinik Biyokimya Laboratuvarında sadece bir kez polikliniğe başvuran hastaların kayıtlı 40.486 bireye ait ASpartatTransaminaz (AST) ölçümleri kullanılmıştır. Aynı veriler kullanılarak parametrik olmayan yöntemle de referans aralık hesabı yapılmıştır. Parametrik olmayan yöntemle referans aralığı %95 güvenle [12.28-49.00] aralığında hesaplanmıştır. Bhattacharya yöntemine göre [7.10-32.26] olarak hesaplanmıştır. Elde edilen sonuçlar karşılaştırıldığında parametrik olmayan yönteme göre Bhattacharya yönteminin referans aralığı daha dar bir aralıkta çıkmıştır.
References
- clpmag.com [Internet].Utah Governor Huntsman recognizes medical laboratory week. Clinical Lab Products. Issue Stories. May 2006. [Updated: 2006 May; http://www.clpmag.com/issues/articles/2006- 05_03.asp. Available from:
- Shirts HB, Wilson AR, Jackson BR. Partitioning reference intervals by use of genetic information. Clinical Chemistry. 2011; 57(3): 475-81.
- Kataye A, Balciza C, Seccombe DW. Establishing reference intervals for clinical laboratory test results. Is there a better way? Clinical chemistry / estimating reference intervals. Am J Clin Pathol. 2010; 133(1): 180-6.
- Clinical and laboratory standards institute. Defining, establishing, and verifying reference intervals in the clinical laboratory; approved guideline. Third Edition. CLSI document C28-A3. Wayne, PA: Clinical and Laboratory Standards Institute; 2008.
- International federation of clinical chemistry and international committee for standardization in hematology: Approved recommendation (1986) on the theory of reference values. Part I. The concept of reference values. J Clin Chem Clin Biochem. 1987; 25(5): 337-42.
- Solberg HE, Grasberck R. Reference values. Adv Clin Chem. 1989; 27(1): 1-79.
- Nagayama I, Yamamoto K, Saito K, Kuzuya T, Saito T. Subject-based reference values in thyroid tests. Endocrine Journal. 1993; 40(5): 557-62.
- Baadenhuijsen H, Smit JC. Indirect estimation of clinical chemical reference intervals from total hospital patient data: Application of a modified bhattacharya procedure. J Clin Chem Clin Biochem. 1985; 23(12): 829-39.
- Bhattacharya CG. A simple method of resolution of a distribution into Gaussian components. Biometrics. 1967; 23(1): 115-35.
- Calleja J. Bhattacharya analysis. AACB SES; 2010; Sydney. http://oldsite.aacb.asn.au/files/File/SES/2010/AQ/1130- 1200%20Calleja.pdf. from:
- Usta M. Koagülasyon testlerinin referans aralıklarının indirekt belirlenmesinde bhattacharya ve hoffman metotlarının kalite kontrol prosedürleri olarak karşılaştırılması [Uzmanlık Tezi]. İstanbul: T.C. Sağlık Bakanlığı İstanbul Eğitim ve Araştırma Hastanesi Klinik Biyokimya Laboratuvarı; 2009.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research Articles |
| Authors | |
| Publication Date | May 31, 2015 |
| Submission Date | July 10, 2014 |
| Published in Issue | Year 2015 Volume: 5 Issue: 2 |
