Abstract
Detection of auto-antibody by Enzyme Linked Immunosorbent Assay (ELISA) technique is used method for diagnosis of autoimmune disease. This study was aimed to compare the measurement of antinuclear antibodies (ANA) and double-stranded DNA (ds DNA) by ELISA in serum samples.
Serum samples were obtained from 2 sources of: sent to the laboratory for ANA testing and sent to the laboratory for dsDNA. Each of these sera was tested for the presence of ANA and dsDNA by ELISA kits. This tests were performed by commercial ‘‘Aeskulisa dsDNA check” (AESKU DIAGNOSTICS, GERMANY) according to the manufacturer's instructions. ANA and ds DNA results were classified as positive or negative for each patient. Borderline results were arbitrarily classified as positive.
In patients, 2,96% (58/1975) were ANA positive and 4,52% (29/642) were ds DNA positive by ELISA. There is no significant correlation between ANA and ds DNA positivity. Only two patient both ANA and ds DNA positivity were found by ELISA kits. Agreement between assays is generally marginal.
There are a number of factors which may contribute to the variability between these assays. The ELISA ANA assay in our laboratory uses a commercial kit utilize an IgG-specifik second antibody. Finally, each of the assays provides different criteria for clinicians and ANA screen test should be followed up with a fluorescent tests to provide information on ANA pattern.
Keywords
References
- Takeuchi Y, Ishikawa O, Miyachi Y. The comparative
- study of anti-double stranded DNA antibody levels
- measured by radioimmunoassay and enzyme-linked immunosorbent assay in systemic lupus erythematosus. J Dermatol 1997; 24: 297-300.
- Krippner H, Merle S, Pirlet K. Enzyme immunoassay for IgG and IgM antibodies against dsDNA and ssDNA. Z Rheumatol 1983; 42: 256-260.
- Ward MM. Laboratory testing for systemic rheumatic diseases. Postgrad Med 1998; 103: 93-100.
- Şimşek B, Mısırlıoğlu H, Ören S, et al. Retrospektif olarak Antinükleer Antikor Pozitif olan hastalarda indirect immunofloresan (İİF) ve İmmünoblotting (İB) test sonuçlarının değerlendirilmesi. 21. Ulusal İmmünoloji Kongresi 2011; 21: 111.
- İlhan F, Akbulut H, Gödekmerdan A. Laboratuvarımızda saptanan ANA paternlerinin retrospektif incelenmesi. 21. İmmünoloji Kongresi 2011; 21: 126.
- Kavanaugh A, Tomar R, Reveille J, Solomon DH, Homburger HA. Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. American College of Pathologists. Arch Pathol Lab Med 2000; 124: 71- 81.
Abstract
References
- Takeuchi Y, Ishikawa O, Miyachi Y. The comparative
- study of anti-double stranded DNA antibody levels
- measured by radioimmunoassay and enzyme-linked immunosorbent assay in systemic lupus erythematosus. J Dermatol 1997; 24: 297-300.
- Krippner H, Merle S, Pirlet K. Enzyme immunoassay for IgG and IgM antibodies against dsDNA and ssDNA. Z Rheumatol 1983; 42: 256-260.
- Ward MM. Laboratory testing for systemic rheumatic diseases. Postgrad Med 1998; 103: 93-100.
- Şimşek B, Mısırlıoğlu H, Ören S, et al. Retrospektif olarak Antinükleer Antikor Pozitif olan hastalarda indirect immunofloresan (İİF) ve İmmünoblotting (İB) test sonuçlarının değerlendirilmesi. 21. Ulusal İmmünoloji Kongresi 2011; 21: 111.
- İlhan F, Akbulut H, Gödekmerdan A. Laboratuvarımızda saptanan ANA paternlerinin retrospektif incelenmesi. 21. İmmünoloji Kongresi 2011; 21: 126.
- Kavanaugh A, Tomar R, Reveille J, Solomon DH, Homburger HA. Guidelines for clinical use of the antinuclear antibody test and tests for specific autoantibodies to nuclear antigens. American College of Pathologists. Arch Pathol Lab Med 2000; 124: 71- 81.
Details
| Primary Language | English |
|---|---|
| Journal Section | Articles |
| Authors | |
| Publication Date | June 2, 2015 |
| Published in Issue | Year 2015 Volume: 20 Issue: 1 |