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Investigation of Lactobacillus spp. bacteria in infants consuming breast milk and formula and determination of some probiotic characters

Year 2023, Volume: 9 Issue: 6 - November 2023, 1334 - 1342, 04.11.2023
https://doi.org/10.18621/eurj.1202118

Abstract

Objectives: It was aimed to investigate some probiotic properties of Lactic Acid Bacteria (LAB) isolated from stool samples taken from 45 healthy 0-4 month old babies, who did not take antibiotics or probiotic supplements in the last 3 months, and who did not have any health problems.

Methods: Six different species were obtained from 21 isolates selected by the method of Mass Spectrometry (MALDI-TOF MS).

Results: The most common strain was Lactobacillus rhamnosus with a rate of 59%, followed by Lactobacillus paracasei with a rate of 13.6%. Vancomycin, tetracycline, gentamicin, netilmicin, tobramycin, penicillin, ampicillin, teicoplanin and amikacin antibiotics were used to evaluate the antimicrobial activities of the strains. In our study, while all strains were resistant to the antibiotic amikacin, they were sensitive to tetracycline, penicillin, gentamicin, netilmicin, teicoplanin, vancomycin, ampicillin and tobramycin antibiotics. In the evaluation of the antagonistic activities of LAB, 6 different pathogens (Escherichia coli ATCC 25922, Staphylococcus aureus ATCC 6538, Bacillus subtilis ATCC 6633, S. aureus ATCC 25923, Candida albicans ATCC 10231, Listeria monocytogenes ATCC 19111) were used and it was determined that the strains showed antimicrobial effects on all pathogenic microorganisms. Cholesterol assimilation abilities, T21 and T22 strains achieved the highest cholesterol assimilation rate of 39.1%.

Conclusions: It is thought that most of the isolated strains have probiotic potential, and especially Lactobacillus gasseri T21 and L. paracasei T22 strains may be probiotic strains that can be used in the production of preparations alone or together with other Lactobacillus strains.

Thanks

I am grateful to Esin KIRAY for providing checking/correction services by examining this document with great care in terms of word choice, style and consistency, as well as grammar, spelling and punctuation.

References

  • 1. Amenyogbe N, Kollmann TR, Ben-Othman R. Early-life host-microbiome interphase: the key frontier for immune development. Front Pediatr 2017;5:111.
  • 2. Tao N, Wu S, Kim J, An HJ, Hinde K, Power ML, et al. Evolutionary glycomics: characterization of milk oligosaccharides in primates. J Proteome Res 2011;10:1548-57.
  • 3. Gorbach SL. Probiotics and gastrointestinal health. Am J Gastroenterol 2000;95:2-4.
  • 4. Yılmaz S, Duyan S, Artuk C, Diktaş H. [Applications of MALDI-TOF MS in microbiological identification]. TAF Preventive Medicine Bulltein 2014;13:421-6. [Article in Turkish]
  • 5. Maragkoudakis PA, Zoumpopoulou G, Miaris C, Kalantzopoulos G, Pot B, Tsakalidou E. Probiotic potential of Lactobacillus strains isolated from dairy products. Int Dairy J 2006;16:189-99.
  • 6. Chung HS, Kim YB, Chun, SL, Ji GE. Screening and selection of acid and bile resistant bifidobacteria. Food Microbiol 1999;47:25-32.
  • 7. Zoral S. İnsan Kaynaklı Lactobacillus spp. Suşlarının Probiyotik Özelliklerinin Belirlenmesi. 2013. YÖK Ulusal Tez Merkezi.
  • 8. Metin M. Süt Teknolojisi. 7. Baskı. Ege Üniversitesi Basımevi. 2008
  • 9. Kurt A. Süt ve Mamülleri Muayene Ve Analiz Metodları Rehberi. (3. Baskı). Atatürk Üniversitesi Basımevi.1984.
  • 10. Caicedo RA,  Schanler RJ, Li N, Neu J. The Developing intestinal ecosystem: implications for the neonate. Pediatr Resource 2005;58:625-8.
  • 11. Martin R, Makino H, Cetinyurek Yavuz A, Ben- Amor K, Roelofs M, Ishikawa E, et al. Early-life events, including mode of delivery and type of feeding, siblings and gender, shape the developing gut microbiota. PLoS One 2016;11:e0158498.
  • 12. Penders J, Thijs C, Vink C, Stelma FF, Snijders B, Kummeling I, et al. Factors influencing the composition of the intestinal microbiota in early infancy. Pediatrics 2016;118:511-21.
  • 13.Underwood MA, Bruce German J, Lebrilla CB, Mills DA. Bifidobacterium longum subspecies infantis: Champion colonizer of the infant gut. Pediatr Res 2015;77:229-35.
  • 14. Rinne M, Kalliomaki M, Arvilommi H, Salminen S, Isolauri E. Effect of probiotics and breastfeeding on the Bifidobacterium and Lactobacillus/Enterococcus microbiota and humoral immune responses. J Pediatr 2005;147:186-91.
  • 15. Ozgun D, Cingilli Vural H. Identification of Lactobacillus strains isolated from faecal specimens of babies and human milk colostrum by API 50 CHL system. J Med Genet Genomics 2011;3:46-49.
  • 16. Reale A, Di Renzo T, Rossi F, Zotta T, Lacumin L, Preziuso M, et al. Tolerance of Lactobacillus casei, Lactobacillus paracasei and Lactobacillus rhamnosus strains to stress factors encountered in food processing and in the gastro-intestinal tract. LWT Food Sci Technol 2015;60:721-8.
  • 17. Kalui MC, Mathara, JM, Kutima PM, Kiiyuka, C, Wongo, LE Functional characteristics of Lactobacillus plantarum and Lactobacillus rhamnosus from Ikii, A Kenyan Traditional Fermented Maize Porridge. Afr J Biotechnol 2009;8:4363-73.
  • 18. Oh NS, Joung JY, Lee JY, Kim Y. Probiotic and anti-inflammatory potential of Lactobacillus rhamnosus 4B15 and Lactobacillus gasseri 4M13 isolated from infant feces, PLoS One 2018;13:e0192021.
  • 19. Mandal V, Sen SK, Mandal NC. Effect of prebiotics on bacteriocin production and cholesterol lowering activity of Pediococcus acidilactici LAB 5. World J Microbiol Biotechnol 2009;25:1837-47.
  • 20. Al Atya AK, Drider- Hadiouche K, Ravallec R, Silvain, A, Vachee A, Drider, D. Probiotic potential of Enterococcus faecalis strains isolated from meconium. Front Microbiol 2015;6 227.
  • 21. Kıray E. İnsan Kaynaklı Vajen Laktik Asit Bakterilerinin Bazı Probiyotik Özelliklerinin Araştırılması. YÖK Ulusal Tez Merkezi. 2017.
  • 22. Tokatlı Demirok N. Bebeklerden İzole Edilen Lactobacillus spp.'nin Fonksiyonel Özelliklerinin Belirlenmesi, YÖK Ulusal Tez Merkezi, 2014.
  • 23. Belviso S, Giordano M, Dolci P, Zeppa, G. In vitro cholesterol-lowering activity of Lactobacillus plantarum and Lactobacillus paracasei strains isolated from the Italian Castelmagno PDO cheese. Dairy Sci Technol 2009;89:169-76.
  • 24. Fugaban JII, Vazquez Bucheli JE, Kim B, Holzapfel WH, Todorov SD. Safety and beneficial properties of bacteriocinogenic Pediococcus acidilactici and Pediococcus pentosaceus isolated from silage. Lett Appl Microbiol 2021;73:725-34.
  • 25. Jan WJ, Kim CE, Hyeon Jeon M, Jeong Lee S, Lee JM, Woo Lee E, et al. Characterization of Pediococcus acidilactici FS2 isolated from Korean traditional fermented seafood and its blood cholesterol reduction effect in mice. J Funct Foods, 2021;87:1-81.
  • 26. Rathnayake IU, Hargreaves M, Huygens F. Antibiotic resistance and virulence traits in clinical and environmental Enterococcus faecalis and Enterococcus faecium isolates. Syst Appl Microbiol 2012;35:326-33.
  • 27. Toğay SO, Keskin AC, Açik L, Temiz, A. Virulence genes, antibiotic resistance and plasmid profiles of Enterococcus faecalis and Enterococcus faecium from naturally fermented Turkish foods. J Appl Microbiol 2010;109:1084-92.
  • 28. Chou LS, Weimer B. Isolation and characterization of acid- and biletolerant isolates from strains of Lactobacillus acidophilus, J Dairy Sci 1999;82:23-31.
  • 29. Castorena-Alba MM, Vázquez-Rodríguez, JA, Cabanillas Lomelí ML, González-Martínez BE. Cholesterol assimilation, acid and bile survival of probiotic bacteria isolated from food and reference strains. CyTA J Food 2017;16 :36-41.
Year 2023, Volume: 9 Issue: 6 - November 2023, 1334 - 1342, 04.11.2023
https://doi.org/10.18621/eurj.1202118

Abstract

References

  • 1. Amenyogbe N, Kollmann TR, Ben-Othman R. Early-life host-microbiome interphase: the key frontier for immune development. Front Pediatr 2017;5:111.
  • 2. Tao N, Wu S, Kim J, An HJ, Hinde K, Power ML, et al. Evolutionary glycomics: characterization of milk oligosaccharides in primates. J Proteome Res 2011;10:1548-57.
  • 3. Gorbach SL. Probiotics and gastrointestinal health. Am J Gastroenterol 2000;95:2-4.
  • 4. Yılmaz S, Duyan S, Artuk C, Diktaş H. [Applications of MALDI-TOF MS in microbiological identification]. TAF Preventive Medicine Bulltein 2014;13:421-6. [Article in Turkish]
  • 5. Maragkoudakis PA, Zoumpopoulou G, Miaris C, Kalantzopoulos G, Pot B, Tsakalidou E. Probiotic potential of Lactobacillus strains isolated from dairy products. Int Dairy J 2006;16:189-99.
  • 6. Chung HS, Kim YB, Chun, SL, Ji GE. Screening and selection of acid and bile resistant bifidobacteria. Food Microbiol 1999;47:25-32.
  • 7. Zoral S. İnsan Kaynaklı Lactobacillus spp. Suşlarının Probiyotik Özelliklerinin Belirlenmesi. 2013. YÖK Ulusal Tez Merkezi.
  • 8. Metin M. Süt Teknolojisi. 7. Baskı. Ege Üniversitesi Basımevi. 2008
  • 9. Kurt A. Süt ve Mamülleri Muayene Ve Analiz Metodları Rehberi. (3. Baskı). Atatürk Üniversitesi Basımevi.1984.
  • 10. Caicedo RA,  Schanler RJ, Li N, Neu J. The Developing intestinal ecosystem: implications for the neonate. Pediatr Resource 2005;58:625-8.
  • 11. Martin R, Makino H, Cetinyurek Yavuz A, Ben- Amor K, Roelofs M, Ishikawa E, et al. Early-life events, including mode of delivery and type of feeding, siblings and gender, shape the developing gut microbiota. PLoS One 2016;11:e0158498.
  • 12. Penders J, Thijs C, Vink C, Stelma FF, Snijders B, Kummeling I, et al. Factors influencing the composition of the intestinal microbiota in early infancy. Pediatrics 2016;118:511-21.
  • 13.Underwood MA, Bruce German J, Lebrilla CB, Mills DA. Bifidobacterium longum subspecies infantis: Champion colonizer of the infant gut. Pediatr Res 2015;77:229-35.
  • 14. Rinne M, Kalliomaki M, Arvilommi H, Salminen S, Isolauri E. Effect of probiotics and breastfeeding on the Bifidobacterium and Lactobacillus/Enterococcus microbiota and humoral immune responses. J Pediatr 2005;147:186-91.
  • 15. Ozgun D, Cingilli Vural H. Identification of Lactobacillus strains isolated from faecal specimens of babies and human milk colostrum by API 50 CHL system. J Med Genet Genomics 2011;3:46-49.
  • 16. Reale A, Di Renzo T, Rossi F, Zotta T, Lacumin L, Preziuso M, et al. Tolerance of Lactobacillus casei, Lactobacillus paracasei and Lactobacillus rhamnosus strains to stress factors encountered in food processing and in the gastro-intestinal tract. LWT Food Sci Technol 2015;60:721-8.
  • 17. Kalui MC, Mathara, JM, Kutima PM, Kiiyuka, C, Wongo, LE Functional characteristics of Lactobacillus plantarum and Lactobacillus rhamnosus from Ikii, A Kenyan Traditional Fermented Maize Porridge. Afr J Biotechnol 2009;8:4363-73.
  • 18. Oh NS, Joung JY, Lee JY, Kim Y. Probiotic and anti-inflammatory potential of Lactobacillus rhamnosus 4B15 and Lactobacillus gasseri 4M13 isolated from infant feces, PLoS One 2018;13:e0192021.
  • 19. Mandal V, Sen SK, Mandal NC. Effect of prebiotics on bacteriocin production and cholesterol lowering activity of Pediococcus acidilactici LAB 5. World J Microbiol Biotechnol 2009;25:1837-47.
  • 20. Al Atya AK, Drider- Hadiouche K, Ravallec R, Silvain, A, Vachee A, Drider, D. Probiotic potential of Enterococcus faecalis strains isolated from meconium. Front Microbiol 2015;6 227.
  • 21. Kıray E. İnsan Kaynaklı Vajen Laktik Asit Bakterilerinin Bazı Probiyotik Özelliklerinin Araştırılması. YÖK Ulusal Tez Merkezi. 2017.
  • 22. Tokatlı Demirok N. Bebeklerden İzole Edilen Lactobacillus spp.'nin Fonksiyonel Özelliklerinin Belirlenmesi, YÖK Ulusal Tez Merkezi, 2014.
  • 23. Belviso S, Giordano M, Dolci P, Zeppa, G. In vitro cholesterol-lowering activity of Lactobacillus plantarum and Lactobacillus paracasei strains isolated from the Italian Castelmagno PDO cheese. Dairy Sci Technol 2009;89:169-76.
  • 24. Fugaban JII, Vazquez Bucheli JE, Kim B, Holzapfel WH, Todorov SD. Safety and beneficial properties of bacteriocinogenic Pediococcus acidilactici and Pediococcus pentosaceus isolated from silage. Lett Appl Microbiol 2021;73:725-34.
  • 25. Jan WJ, Kim CE, Hyeon Jeon M, Jeong Lee S, Lee JM, Woo Lee E, et al. Characterization of Pediococcus acidilactici FS2 isolated from Korean traditional fermented seafood and its blood cholesterol reduction effect in mice. J Funct Foods, 2021;87:1-81.
  • 26. Rathnayake IU, Hargreaves M, Huygens F. Antibiotic resistance and virulence traits in clinical and environmental Enterococcus faecalis and Enterococcus faecium isolates. Syst Appl Microbiol 2012;35:326-33.
  • 27. Toğay SO, Keskin AC, Açik L, Temiz, A. Virulence genes, antibiotic resistance and plasmid profiles of Enterococcus faecalis and Enterococcus faecium from naturally fermented Turkish foods. J Appl Microbiol 2010;109:1084-92.
  • 28. Chou LS, Weimer B. Isolation and characterization of acid- and biletolerant isolates from strains of Lactobacillus acidophilus, J Dairy Sci 1999;82:23-31.
  • 29. Castorena-Alba MM, Vázquez-Rodríguez, JA, Cabanillas Lomelí ML, González-Martínez BE. Cholesterol assimilation, acid and bile survival of probiotic bacteria isolated from food and reference strains. CyTA J Food 2017;16 :36-41.
There are 29 citations in total.

Details

Primary Language English
Subjects Medical Microbiology
Journal Section Original Articles
Authors

Tuğçe Muslu Çağal 0000-0003-0161-9510

Esin Kıray 0000-0002-6908-5909

Ergin Kariptaş 0000-0001-6513-9589

Early Pub Date May 29, 2023
Publication Date November 4, 2023
Submission Date November 10, 2022
Acceptance Date February 13, 2023
Published in Issue Year 2023 Volume: 9 Issue: 6 - November 2023

Cite

AMA Muslu Çağal T, Kıray E, Kariptaş E. Investigation of Lactobacillus spp. bacteria in infants consuming breast milk and formula and determination of some probiotic characters. Eur Res J. November 2023;9(6):1334-1342. doi:10.18621/eurj.1202118

e-ISSN: 2149-3189 


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