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Evaluation of Antimicrobial Activities of Various Herbal Oils Against Helicobacter pylori And Their Cytotoxic Effects On HUVEC Cell Line

Year 2023, Volume: 51 Issue: 1, 103 - 113, 01.01.2023

Tuğçe Deniz Karaca Başak Aru Kayhan Çağlar

https://doi.org/10.15671/hjbc.1114101

Abstract

Helicobacter pylori (H. pylori) enfeksiyonu en önemli kronik bakteriyel enfeksiyon olarak kabul edilmektedir. Son yıllarda tedavide kullanılan antibiyotiklere karşı bakterinin direnç geliştirdiği rapor edilmiştir. Tedavide başarı oranının yükselmesi ve rekürrensin azalması için, toksik olmayan biyouyumlu bitkisel kaynakların araştırılarak sistemik antibiyotik tedavisine ek olarak kullanılması ve eradikasyonun sağlanması son derece önemlidir. Bitkilerden elde edilen yağlar uzun yıllardan beri çeşitli amaçlara yönelik, özellikle ticari ve bilimsel alanlarda kullanılmaktadır. Bu nedenle çalışmamızda; genellikle mide ve gastrointestinal sistem rahatsızlıklarına iyi geldiği bilinen ve literatürde H.pyloriüzerinde yapılmış yeterli çalışması bulunmayan çeşitli bitkisel yağlar (Eucalyptus globulus, Juniperus communis, Rosmarinus officinalis, Thymus vulgaris) seçilmiş ve H. pylori'ye karşı antimikrobiyal etkinlikleri ile Human Umbilical Vein Endothelial Cells (HUVEC) hücre soyu üzerindeki sitotoksik etkilerinin araştırılması amaçlanmıştır. Bitkisel yağların antimikrobiyal etkinlikleri mikrodilüsyon yöntemi ile (MIC, MBC), sitotoksik etkinlikleri ise MTT ve LDH yöntemleri ile incelenmiştir. H.pylori’ye karşı antimikrobiyal etkisini araştırdığımız, Eucalyptus globulus (MIC: 2,81 %v/v, MBC: 5,62 %v/v), Juniperus communis (MIC: 0,35 %v/v, MBC: 0,70 %v/v), Rosmarinus officinalis (MIC: 2,81 %v/v, MBC: 5,62 %v/v) ve Thymus vulgaris (MIC: 0,70 %v/v, MBC: 1,40 %v/v) 'in etkili olduğu gözlemlenmiştir. Bunun yanında HUVEC hücre soyu üzerinde Thymus vulgaris bitkisel yağının en yüksek, Eucalyptus globulus bitkisel yağının ise en düşük sitotoksik etkiye sahip olduğu tespit edilmiştir.
Anahtar Kelimeler: Helicobacter pylori, Bitkisel yağ, Antibakteriyel aktivite, Sitotoksisite

Keywords

Keywords: Helicobacter pylori Herbal oil Antibacterial activity Cytotoxicity

References

  • Referans 1. Falush, D., Wirth, T., Pritchard, J.K., Stephens, M., Kidd, M., Blaser, M.J., Graham, D.Y., Vacher, S., Perez-Perez, G.I., Yamaoka, Y., Megraud, F., Otto, K., Reichard, U., Katzowitsch, E., Wang, X., Achtman, M. and Suerbaum, S, Traces of human migrations in Helicobacter pylori populations, Science, (2003) 1582-1585.
  • Referans 2. Goodwin, S.C., Armstrong, J.A., Chilvers, T., Peters, M., Collins, M.D., Sly, L., Mcconnell, W. and Harper, W.E.S, Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb. Nov. Respectively, International Journal of Systematic Bacteriology, (1989) 397-405.
  • Referans 3. Marshall, B.J. and Warren, J.R, Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration, The Lancet, (1984) 1311-1315.
  • Referans 4. Hopkins, R.J. and Morris, J.G, Helicobacter pylori: The missing link in perspective. The American Journal of Medicine, (1994) 265-277.
  • Referans 5. Suerbaum, S. and Michetti, P., Helicobacter pylori infection. The New England Journal of Medicine, (2002) 1175-1186.
  • Referans 6. Kidd, M., and Modlin, I.M, A century of Helicobacter pylori, Gastric Surgical Pathobiology Research, (1998) 1-15.
  • Referans 7. Luck, J.M., and Seth, T.N, The physiology of gastric urease, Biochemical Journal, (1925) 357-365.
  • Referans 8. Suerbaum, S. and Michetti, P, Helicobacter pylori infection, The New England Journal of Medicine, (2002) 1175-1186.
  • Referans 9. Gökçekuyu, B.M, Helicobacter pylori eradikasyonunda birinci basamak tedavide kullanılan antibiyotiklerin direnç durumları, Doktora Tezi, Dokuz Eylül Üniversitesi Tıp Fakültesi, İzmir, (2011).
  • Referans 10. Shames, B., Krajden, S., Fuksa, M., Babida, C., Penner, J.L, Evidence for the occurrence of the same strain of Campylobacter pylori in the stomach and dental plaque, Journal of Clinical Microbiology, (1989) 2849-2850.
  • Referans 11. Majmudar, P., Shah, S.M., Dhunjibhoy, K.R. and Desai, H.G, Isolation of Helicobacter pylori from dental plaques in healthy volunteers, Indian Journal of Gastroenterology, (1990) 271-272.
  • Referans 12. Dowsett, S.A., Archila, L., Segreto, V.A., Gonzales, C.R., Silva, A., Vastola, K.A., Bartizek, R.D. and Kowolik, M.J, Helicobacter pylori: Infection in ındigenous families of Central America: Serostatus and oral and fingernail carriage, Journal of Clinical Microbiology, (1999) 2456–2460.
  • Referans 13. Oshowo, A., Gillam, D., Botha, A., Tunio, M., Holton, J., Boulos, P. and Hobsley, M, Helicobacter pylori: The mouth, stomach, and gut axis, Ann Periodontol, (1998) 276-280.
  • Referans 14. Socransky, S.S. and Haffajee, A.D, Dental biofilms: Difficult therapeutic targets, Periodontolog 2000, (2002) 12–55.
  • Referans 15. Valdivieso-Ugarte, M, Gomez-Llorente, C., Plaza-Díaz, J., Gil, Á, Antimicrobial, Antioxidant, and Immunomodulatory Properties of Essential Oils: A Systematic Review, (2019) 2786.
  • Referans 16. D’agostino, M., Tesse, N., Frippiat, J.P., Machouart, M., Debourgogne, A, Essential Oils and Their Natural, Active Compounds Presenting Antifungal Properties, Molecules, (2019) 3713.
  • Referans 17. Çelik, E. ve Yuvalı Çelik, G, Bitki uçucu yağlarının antimikrobiyal özellikleri, Orlab On-Line Mikrobiyoloji Dergisi, (2007) 1-6.
  • Referans 18. Cogo, L.L., Monteiro, C.L.B., Miguel, M.D., Miguel, O.G., Cunico, M.M., Ribeiro, M.L., Camargo, E.R., Kussen, G.M.B., Nogueira, K.S., and Costa, L.M.D, Anti-Helicobacter pylori activity of plant extracts traditionally used for the treatment of gastrointestinal disorders, Brazilian Journal of Microbiology, (2010) 304–309.
  • Referans 19. Kıvanç, M. ve Akgül, A, Antibacterial activities of essential oils from turkish spices and citrus, Flavour and Fragrance Journal, (1986) 175-179.
  • Referans 20. Sakkas, H., Gousia, P., Economou, V., Sakkas, V., Petsios, S. and Papadopoulou, C, In vitro antimicrobial activity of five essential oils on multidrug resistant Gram-negative clinical isolates, Journal of Intercultural Ethnopharmacology, (2016) 212-218.
  • Referans 21. Güzeldağ, G., Kadioglu, L., Mercimek, A. and Matyar, F, Preliminary examination of herbal extracts on the inhibition of Helicobacter pylori, African Journal of Traditional Complementary and Alternative Medicines, (2014) 93-96.
  • Referans 22. Ali, S.M., Khan, A.A., Ahmed, I., Musaddiq, M., Ahmed, K.S., Polasa, H., Rao, L. V., Habibullah, C. M., Sechi, L.A. ve Ahmed, N, Antimicrobial activities of Eugenol and Cinnamaldehyde against the human gastric pathogen Helicobacter pylori, Annals of Clinical Microbiology and Antimicrobials, (2005) 1-7.
  • Referans 23. Fotakis, G. and Timbrell, J.A, In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride, Toxicol Lett., (2006) 171-7.
  • Referans 24. Berridge, M.V., Herst, P.M. and Tan, A.S, Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction, Biotechnology Annual Review,(2005) 127-152.
  • Referans 25. Su-Wen, L., Hwei-San, L., Kang-Nee, T., Tracey, D. B. and, Reduction of MTT to Purple Formazan by Vitamin E Isomers in the Absence of Cells, Tropical Life Sciences Research, (2015) 111–120.
  • Referans 26. Sanjeev, K. M., Prakash, C. and Seog-Woo, R, In vitro Models, Endpoints and Assessment Methods for the Measurement of Cytotoxicity, Toxicology and Environmental Health Sciences, (2010) 87-93.
  • Referans 27. Esmaili, D., Mobarez, A.M. and Tohidpour, A, Anti-Helicobacter pylori activities of shoya powder and essential oils of Thymus vulgaris and Eucalyptus globulus, The Open Microbiology Journal, (2012) 65-69.
  • Referans 28. Mahady, G.B., Pendland, S.L., Stoia, A., Hamill, F.A., Fabricant, D., Dietz, B.M. and Chadwick, L.R, In vitro susceptibilityof Helicobacter pylori to botanical extracts used traditionally fort he treatment of gastrointestinal disorders, Phytotherapy Research, (2005) 988-991.
  • Referans 29. Cabral, C., Francisco, V., Cavaleiro, C., Gonçalves, M.J., Cruz, M.T., Sales, F., Batista, M.T. and Salgueiro, L, Essential oil of Juniperus communis subsp. alpina (Suter) Čelak needles. chemical composition, antifungal activity and cytotoxicity, Phytotherapy Research, (2012) 1352-1357.
  • Referans 30. Döll-Boscardin, P.M., Sartoratto, A., Maia, B.H.L.N.S., Paula, J.P., Nakashima, T., Farago, P.V. and Kanunfre, C.C, In Vitro Cytotoxic Potential of Essential Oils of Eucalyptus benthamii and Its Related Terpenes on Tumor Cell Lines, Evidence-Based Complementary and Alternative Medicine, (2012) 1-8.
  • Referans 31. Cattaneo, L., Cicconi, R., Mignogna, G., Giorgi, A., Mattei, M., Graziani, G., Ferracane, R., Grosso, A., Aducci, P., Schininà, M.E. and Marra, M, Anti- Proliferative Effect of Rosmarinus officinalis L. Extract on Human Melanoma A375 Cells. Plos One, (2015) 1-18.
  • Referans 32. Ayesh, B.M., Abed, A.A. and Faris, D.M, In vitro inhibition of human leukemia THP-1 cells by Origanum syriacum L. and Thymus vulgaris L. Extracts, BMC Research Notes, (2014) 1-6.

Year 2023, Volume: 51 Issue: 1, 103 - 113, 01.01.2023

Tuğçe Deniz Karaca Başak Aru Kayhan Çağlar

https://doi.org/10.15671/hjbc.1114101

Abstract

References

  • Referans 1. Falush, D., Wirth, T., Pritchard, J.K., Stephens, M., Kidd, M., Blaser, M.J., Graham, D.Y., Vacher, S., Perez-Perez, G.I., Yamaoka, Y., Megraud, F., Otto, K., Reichard, U., Katzowitsch, E., Wang, X., Achtman, M. and Suerbaum, S, Traces of human migrations in Helicobacter pylori populations, Science, (2003) 1582-1585.
  • Referans 2. Goodwin, S.C., Armstrong, J.A., Chilvers, T., Peters, M., Collins, M.D., Sly, L., Mcconnell, W. and Harper, W.E.S, Transfer of Campylobacter pylori and Campylobacter mustelae to Helicobacter gen. nov. as Helicobacter pylori comb. nov. and Helicobacter mustelae comb. Nov. Respectively, International Journal of Systematic Bacteriology, (1989) 397-405.
  • Referans 3. Marshall, B.J. and Warren, J.R, Unidentified curved bacilli in the stomach of patients with gastritis and peptic ulceration, The Lancet, (1984) 1311-1315.
  • Referans 4. Hopkins, R.J. and Morris, J.G, Helicobacter pylori: The missing link in perspective. The American Journal of Medicine, (1994) 265-277.
  • Referans 5. Suerbaum, S. and Michetti, P., Helicobacter pylori infection. The New England Journal of Medicine, (2002) 1175-1186.
  • Referans 6. Kidd, M., and Modlin, I.M, A century of Helicobacter pylori, Gastric Surgical Pathobiology Research, (1998) 1-15.
  • Referans 7. Luck, J.M., and Seth, T.N, The physiology of gastric urease, Biochemical Journal, (1925) 357-365.
  • Referans 8. Suerbaum, S. and Michetti, P, Helicobacter pylori infection, The New England Journal of Medicine, (2002) 1175-1186.
  • Referans 9. Gökçekuyu, B.M, Helicobacter pylori eradikasyonunda birinci basamak tedavide kullanılan antibiyotiklerin direnç durumları, Doktora Tezi, Dokuz Eylül Üniversitesi Tıp Fakültesi, İzmir, (2011).
  • Referans 10. Shames, B., Krajden, S., Fuksa, M., Babida, C., Penner, J.L, Evidence for the occurrence of the same strain of Campylobacter pylori in the stomach and dental plaque, Journal of Clinical Microbiology, (1989) 2849-2850.
  • Referans 11. Majmudar, P., Shah, S.M., Dhunjibhoy, K.R. and Desai, H.G, Isolation of Helicobacter pylori from dental plaques in healthy volunteers, Indian Journal of Gastroenterology, (1990) 271-272.
  • Referans 12. Dowsett, S.A., Archila, L., Segreto, V.A., Gonzales, C.R., Silva, A., Vastola, K.A., Bartizek, R.D. and Kowolik, M.J, Helicobacter pylori: Infection in ındigenous families of Central America: Serostatus and oral and fingernail carriage, Journal of Clinical Microbiology, (1999) 2456–2460.
  • Referans 13. Oshowo, A., Gillam, D., Botha, A., Tunio, M., Holton, J., Boulos, P. and Hobsley, M, Helicobacter pylori: The mouth, stomach, and gut axis, Ann Periodontol, (1998) 276-280.
  • Referans 14. Socransky, S.S. and Haffajee, A.D, Dental biofilms: Difficult therapeutic targets, Periodontolog 2000, (2002) 12–55.
  • Referans 15. Valdivieso-Ugarte, M, Gomez-Llorente, C., Plaza-Díaz, J., Gil, Á, Antimicrobial, Antioxidant, and Immunomodulatory Properties of Essential Oils: A Systematic Review, (2019) 2786.
  • Referans 16. D’agostino, M., Tesse, N., Frippiat, J.P., Machouart, M., Debourgogne, A, Essential Oils and Their Natural, Active Compounds Presenting Antifungal Properties, Molecules, (2019) 3713.
  • Referans 17. Çelik, E. ve Yuvalı Çelik, G, Bitki uçucu yağlarının antimikrobiyal özellikleri, Orlab On-Line Mikrobiyoloji Dergisi, (2007) 1-6.
  • Referans 18. Cogo, L.L., Monteiro, C.L.B., Miguel, M.D., Miguel, O.G., Cunico, M.M., Ribeiro, M.L., Camargo, E.R., Kussen, G.M.B., Nogueira, K.S., and Costa, L.M.D, Anti-Helicobacter pylori activity of plant extracts traditionally used for the treatment of gastrointestinal disorders, Brazilian Journal of Microbiology, (2010) 304–309.
  • Referans 19. Kıvanç, M. ve Akgül, A, Antibacterial activities of essential oils from turkish spices and citrus, Flavour and Fragrance Journal, (1986) 175-179.
  • Referans 20. Sakkas, H., Gousia, P., Economou, V., Sakkas, V., Petsios, S. and Papadopoulou, C, In vitro antimicrobial activity of five essential oils on multidrug resistant Gram-negative clinical isolates, Journal of Intercultural Ethnopharmacology, (2016) 212-218.
  • Referans 21. Güzeldağ, G., Kadioglu, L., Mercimek, A. and Matyar, F, Preliminary examination of herbal extracts on the inhibition of Helicobacter pylori, African Journal of Traditional Complementary and Alternative Medicines, (2014) 93-96.
  • Referans 22. Ali, S.M., Khan, A.A., Ahmed, I., Musaddiq, M., Ahmed, K.S., Polasa, H., Rao, L. V., Habibullah, C. M., Sechi, L.A. ve Ahmed, N, Antimicrobial activities of Eugenol and Cinnamaldehyde against the human gastric pathogen Helicobacter pylori, Annals of Clinical Microbiology and Antimicrobials, (2005) 1-7.
  • Referans 23. Fotakis, G. and Timbrell, J.A, In vitro cytotoxicity assays: comparison of LDH, neutral red, MTT and protein assay in hepatoma cell lines following exposure to cadmium chloride, Toxicol Lett., (2006) 171-7.
  • Referans 24. Berridge, M.V., Herst, P.M. and Tan, A.S, Tetrazolium dyes as tools in cell biology: new insights into their cellular reduction, Biotechnology Annual Review,(2005) 127-152.
  • Referans 25. Su-Wen, L., Hwei-San, L., Kang-Nee, T., Tracey, D. B. and, Reduction of MTT to Purple Formazan by Vitamin E Isomers in the Absence of Cells, Tropical Life Sciences Research, (2015) 111–120.
  • Referans 26. Sanjeev, K. M., Prakash, C. and Seog-Woo, R, In vitro Models, Endpoints and Assessment Methods for the Measurement of Cytotoxicity, Toxicology and Environmental Health Sciences, (2010) 87-93.
  • Referans 27. Esmaili, D., Mobarez, A.M. and Tohidpour, A, Anti-Helicobacter pylori activities of shoya powder and essential oils of Thymus vulgaris and Eucalyptus globulus, The Open Microbiology Journal, (2012) 65-69.
  • Referans 28. Mahady, G.B., Pendland, S.L., Stoia, A., Hamill, F.A., Fabricant, D., Dietz, B.M. and Chadwick, L.R, In vitro susceptibilityof Helicobacter pylori to botanical extracts used traditionally fort he treatment of gastrointestinal disorders, Phytotherapy Research, (2005) 988-991.
  • Referans 29. Cabral, C., Francisco, V., Cavaleiro, C., Gonçalves, M.J., Cruz, M.T., Sales, F., Batista, M.T. and Salgueiro, L, Essential oil of Juniperus communis subsp. alpina (Suter) Čelak needles. chemical composition, antifungal activity and cytotoxicity, Phytotherapy Research, (2012) 1352-1357.
  • Referans 30. Döll-Boscardin, P.M., Sartoratto, A., Maia, B.H.L.N.S., Paula, J.P., Nakashima, T., Farago, P.V. and Kanunfre, C.C, In Vitro Cytotoxic Potential of Essential Oils of Eucalyptus benthamii and Its Related Terpenes on Tumor Cell Lines, Evidence-Based Complementary and Alternative Medicine, (2012) 1-8.
  • Referans 31. Cattaneo, L., Cicconi, R., Mignogna, G., Giorgi, A., Mattei, M., Graziani, G., Ferracane, R., Grosso, A., Aducci, P., Schininà, M.E. and Marra, M, Anti- Proliferative Effect of Rosmarinus officinalis L. Extract on Human Melanoma A375 Cells. Plos One, (2015) 1-18.
  • Referans 32. Ayesh, B.M., Abed, A.A. and Faris, D.M, In vitro inhibition of human leukemia THP-1 cells by Origanum syriacum L. and Thymus vulgaris L. Extracts, BMC Research Notes, (2014) 1-6.
There are 32 citations in total.

Details

Primary Language English
Subjects Engineering
Journal Section Research Article
Authors

Tuğçe Deniz Karaca 0000-0002-7471-1759

Başak Aru 0000-0002-2987-0523

Kayhan Çağlar 0000-0001-7257-6453

Publication Date January 1, 2023
Acceptance Date June 12, 2022
Published in Issue Year 2023 Volume: 51 Issue: 1

Cite

APA Karaca, T. D., Aru, B., & Çağlar, K. (2023). Evaluation of Antimicrobial Activities of Various Herbal Oils Against Helicobacter pylori And Their Cytotoxic Effects On HUVEC Cell Line. Hacettepe Journal of Biology and Chemistry, 51(1), 103-113. https://doi.org/10.15671/hjbc.1114101
AMA Karaca TD, Aru B, Çağlar K. Evaluation of Antimicrobial Activities of Various Herbal Oils Against Helicobacter pylori And Their Cytotoxic Effects On HUVEC Cell Line. HJBC. January 2023;51(1):103-113. doi:10.15671/hjbc.1114101
Chicago Karaca, Tuğçe Deniz, Başak Aru, and Kayhan Çağlar. “Evaluation of Antimicrobial Activities of Various Herbal Oils Against Helicobacter Pylori And Their Cytotoxic Effects On HUVEC Cell Line”. Hacettepe Journal of Biology and Chemistry 51, no. 1 (January 2023): 103-13. https://doi.org/10.15671/hjbc.1114101.
EndNote Karaca TD, Aru B, Çağlar K (January 1, 2023) Evaluation of Antimicrobial Activities of Various Herbal Oils Against Helicobacter pylori And Their Cytotoxic Effects On HUVEC Cell Line. Hacettepe Journal of Biology and Chemistry 51 1 103–113.
IEEE T. D. Karaca, B. Aru, and K. Çağlar, “Evaluation of Antimicrobial Activities of Various Herbal Oils Against Helicobacter pylori And Their Cytotoxic Effects On HUVEC Cell Line”, HJBC, vol. 51, no. 1, pp. 103–113, 2023, doi: 10.15671/hjbc.1114101.
ISNAD Karaca, Tuğçe Deniz et al. “Evaluation of Antimicrobial Activities of Various Herbal Oils Against Helicobacter Pylori And Their Cytotoxic Effects On HUVEC Cell Line”. Hacettepe Journal of Biology and Chemistry 51/1 (January 2023), 103-113. https://doi.org/10.15671/hjbc.1114101.
JAMA Karaca TD, Aru B, Çağlar K. Evaluation of Antimicrobial Activities of Various Herbal Oils Against Helicobacter pylori And Their Cytotoxic Effects On HUVEC Cell Line. HJBC. 2023;51:103–113.
MLA Karaca, Tuğçe Deniz et al. “Evaluation of Antimicrobial Activities of Various Herbal Oils Against Helicobacter Pylori And Their Cytotoxic Effects On HUVEC Cell Line”. Hacettepe Journal of Biology and Chemistry, vol. 51, no. 1, 2023, pp. 103-1, doi:10.15671/hjbc.1114101.
Vancouver Karaca TD, Aru B, Çağlar K. Evaluation of Antimicrobial Activities of Various Herbal Oils Against Helicobacter pylori And Their Cytotoxic Effects On HUVEC Cell Line. HJBC. 2023;51(1):103-1.
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