Research Article
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The Relationship of Coenzyme Q10, Myo Inositol, Astaxanthin and L-Arginine Values in Serum and Follicular Fluid with Icsi+Et and Pregnancy Outcomes

Year 2023, Volume: 20 Issue: 1, 208 - 217, 27.04.2023
https://doi.org/10.35440/hutfd.1255531

Abstract

Background: The purpose of this study was to evaluate the effects of Coenzyme Q10, Myo-Inositol, Astaxanthin, and L-Arginine levels in the follicular fluid obtained during serum and oocyte collection on oocyte maturation, fertilization, and pregnancy outcomes. Additionally, we aimed to assess the correlation between serum and folli-cular fluid levels and other parameters simultaneously. If significant results are obtained, an affordable and easily accessible test that predicts the success of assisted reproductive technology will be found, resulting in higher treatment success rates and healthier pregnancies.
Materials and Methods: We measured Coenzyme Q10, Myo-Inositol, Astaxanthin, and L-Arginine values in the serum on Day-3 and OPU days, as well as the follicular fluids collected from 60 patients who underwent IVF proto-col at our clinic between October 2021 and October 2022. We statistically evaluated the relationship between Coenzyme Q10, Myo-Inositol, Astaxanthin, and L-Arginine levels with oocyte maturation, fertilization, and preg-nancy outcomes.
Results: We found no correlation between the number of mature oocytes and fertilized oocytes and the values of Coenzyme Q10, Myo-Inositol, Astaxanthin, and L-Arginine measured from serum and follicle (p>0.05). A negative correlation was observed between total gonadotropin doses and the number of collected oocytes, mature oocy-tes, and fertilized oocytes (p<0.05). There was no significant difference in the serum taken on the 3rd day of the follicular phase, in the serum on the day of oocyte retrieval, and in Coenzyme Q10, Myo-Inositol, and L-Arginine values measured from the follicle between pregnant and non-pregnant women (p>0.05). When the Astaxanthin value measured in the serum on the 3rd day of the follicular phase was ≤ 0.3035, the probability of pregnancy was found to be higher. No significant difference was found between clinical pregnancy and biochemical preg-nancy in Coenzyme Q10, Astaxanthin, and L-Arginine values measured in the serum taken on the 3rd day of the follicular phase, in the serum on the day of oocyte retrieval, and in the follicle (p>0.05). The possibility of bioche-mical pregnancy was found to be higher when the Myo-Inositol value measured in serum on the day of oocyte retrieval was ≥ 1.6555.
Conclusions: For stronger results of our research hypothesis, studies with larger patient populations are needed.

Key Words: Follicle fluid, Serum, Infertility, IVF, Coenzyme Q10, Myoinositol, Asta Xanthine, L-Arginine

Project Number

Harran Üniversitesi Bilimsel Araştırma Projeler Koordinatörlüğü (HÜBAK) 22079 nolu projesi

References

  • 1. Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 2005 Jul 14;3:28.
  • 2. Pierce JD, Cackler AB, Arnett MG. Why should you care about free radicals? RN. 2004;67:38–42. quiz 43.
  • 3. Malamitsi-Puchner A, Sarandakou A, Baka S. Concentrations ofangiogenic factors in follicular fluid and oocyte-cumulus complex culture medium from women undergoing in vitro fertilization. Association with oocyte maturity and fertiliza-tion. Fertil Steril 2001;76:98–101.
  • 4. Onalan G, Selam B, Baran Y. Serum and follicular fluid levels of soluble Fas, soluble Fas ligand and apoptosis of luteinized granulosa cells in PCOS patients undergoing IVF. Hum Reprod 2005;20(9):2391–5
  • 5. Guerin P, El Mouatassim S, Menezo Y. Oxidative stress and protection against reactive oxygen species in the pre-implantation embriyo and its surroundings. Hum Reprod Up-date 2001;7:175-89.
  • 6. Sarandakou A, Malamitsi-Puchner A, Baka S, Rizos D, Hassia-kos D, Creatsas G. Apoptosis and proliferation factors in se-rum and follicular fluid from women undergoing in vitro ferti-lization. Fertil Steril 2003;79:634–6
  • 7. Al-Saleh I, Coskun S, Al-Rouqi R, Al-Rajudi T, Eltabache C, Abduljabbar M, Al-Hassan S. Oxidative stress and DNA dama-ge status in couples undergoing in vitro fertilization treat-ment. Reprod Fertil. 2021 May 18;2(2):117-139. doi: 10.1530/RAF-20-0062.
  • 8. Showell MG, Mackenzie-Proctor R, Jordan V, Hart RJ. Anti-oxidants for female subfertility. Cochrane Database Syst Rev. 2020 Aug 27;8(8):CD007807. doi: 10.1002/14651858.CD007807.pub4.
  • 9. Baker VL, Luke B, Brown MB, Alvero R, Frattarelli JL, Usadi R, Grainger DA, Armstrong AY. Multivariate analysis of factors affecting probability of pregnancy and live birth with in vitro fertilization: an analysis of the Society for Assisted Reproduc-tive Technology Clinic Outcomes Reporting System. Fertil Ste-ril. 2010 Sep;94(4):1410-1416. doi: 10.1016/j.fertnstert.2009.07.986. Epub 2009 Sep 9. PMID: 19740463.
  • 10. Romero S, Pella R, Zorrilla I, Berrío P, Escudero F, Pérez Y, García M, Gonzalez C, Orihuela P. Coenzyme Q10 improves the in vitro maturation of oocytes exposed to the intrafollicu-lar environment of patients on fertility treatment. JBRA Assist Reprod. 2020 Jul 14;24(3):283-288.
  • 11. Turi A, Giannubilo SR, Brugè F, Principi F, Battistoni S, Santoni F, Tranquilli AL, Littarru G, Tiano L. Coenzyme Q10 content in follicular fluid and its relationship with oocyte fertilization and embryo grading. Arch Gynecol Obstet. 2012 Apr;285(4):1173-6.
  • 12. Akarsu S, Gode F, Isik AZ, Dikmen ZG, Tekindal MA. The asso-ciation between coenzyme Q10 concentrations in follicular fluid with embryo morphokinetics and pregnancy rate in as-sisted reproductive techniques. J Assist Reprod Genet. 2017 May;34(5):599-605. doi: 10.1007/s10815-017-0882-x. Epub 2017 Feb 9. Erratum in: J Assist Reprod Genet. 2017 May;34(5):607.
  • 13. Boots CE, Boudoures A, Zhang W, Drury A, Moley KH. Obe-sity-induced oocyte mitochondrial defects are partially pre-vented and rescued by supplementation with co-enzyme Q10 in a mouse model. Hum Reprod. 2016 Sep;31(9):2090-7. doi: 10.1093/humrep/dew181. Epub 2016 Jul 17.
  • 14. Alaa Abdulateef Mohammed , Zainab Hassan Al-Khafajy. Ass. Prof Dr. Wasan Adnan Abdullhameed. Coenzyme Q10 in the Follicular Fluid and Its Relation to Oocyte Maturity, Fertiliza-tion Rate, Embryo Grading, and Pregnancy Rate. Indian Jour-nal of Forensic Medicine & Toxicology, January-March 2021, Vol. 15, No. 1 753
  • 15. Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in hu-man fluids. J Clin Pathol. 2001 May;54(5):356-61.
  • 16. Chiu TT, Rogers MS, Law EL, Briton-Jones CM, Cheung LP, Haines CJ. Follicular fluid and serum concentrations of myo-inositol in patients undergoing IVF: relationship with oocyte quality. Hum Reprod. 2002 Jun;17(6):1591-6.
  • 17. Downes CP. Twenty-fifth Colworth medal lecture. The cellu-lar functions of myo-inositol. Biochem Soc Trans. 1989 Apr;17(2):259-68.
  • 18. Gharaei R, Alyasin A, Mahdavinezhad F, Samadian E, Ashraf-nezhad Z, Amidi F. Randomized controlled trial of astaxanthin impacts on antioxidant status and assisted reproductive technology outcomes in women with polycystic ovarian synd-rome. J Assist Reprod Genet. 2022 Apr;39(4):995-1008.
  • 19. Chelenga M, Sakaguchi K, Kawano K, Furukawa E, Yanagawa Y, Katagiri S, Nagano M. Low oxygen environment and as-taxanthin supplementation promote the developmental competence of bovine oocytes derived from early antral fol-licles during 8 days of in vitro growth in a gas-permeable cul-ture device. Theriogenology. 2022 Jan 1;177:116-126.
  • 20. Battaglia C, Regnani G, Marsella T, Facchinetti F, Volpe A, Venturoli S, Flamigni C. Adjuvant L-arginine treatment in controlled ovarian hyperstimulation: a double-blind, rando-mized study. Hum Reprod. 2002 Mar;17(3):659-65.
  • 21. Bodis J, Farkas B, Nagy B, Kovacs K, Sulyok E. The Role of L-Arginine-NO System in Female Reproduction: A Narrative Re-view. Int J Mol Sci. 2022 Nov 28;23(23):14908.
  • 22. Aydogan Mathyk B, Aslan Cetin B, Vardagli D, Zengin E, Sofi-yeva N, Irez T, Ocal P. Comparison of antagonist mild and long agonist protocols in terms of follicular fluid total antioxidant capacity. Taiwan J Obstet Gynecol. 2018;57(2):194-199,

Serum ve Foliküler Sıvıdaki Koenzim Q10, Myoinositol, Asta Ksantin ve L-Arginin Değerlerinin Icsı+Et ve Gebelik Sonuçları ile İlişkisi

Year 2023, Volume: 20 Issue: 1, 208 - 217, 27.04.2023
https://doi.org/10.35440/hutfd.1255531

Abstract

ÖZET

Serum ve Foliküler Sıvıdaki Koenzim Q10, Myoinositol, Asta Ksantin ve L-Arginin
Değerlerinin Icsı+Et ve Gebelik Sonuçları ile İlişkisi


Amaç: Bu çalışmada, serumda ve oosit toplanması sırasında elde edilen folikül sıvısında Koenzim Q10, Myoinositol, Asta Ksantin ve L-Arginin değerlerinin oosit maturasyonu, fertilizasyon ve gebelik sonuçları üzerine etkilerini değerlendirmeyi, ayrıca eş zamanlı olarak serum ve foliküler sıvıdaki seviyeler ve diğer parametreler arasındaki korelasyonun değerlendirilmesi amaçlanmıştır. Bu değerlendirmede önemli sonuçlar elde edilir ise, yardımcı üreme teknolojisinin başarısını öngören uygun fiyatlı ve kolay erişilebilir bir test bulunacak, sağlıklı bir gebelikle sonuçlanan tedavi başarı oranları artacaktır.

Gereç ve Yöntem: Ekim 2021 - Ekim 2022 tarihleri arasında kliniğimizde IVF protokolüne alınan 60 hastanın Gün-3 ile OPU günü alınan serumlarında ve toplanan folliküler sıvılarında Koenzim Q10, Myoinositol, Asta Ksantin ve L-Arginin değerleri çalışıldı. Koenzim Q10, Myoinositol, Asta Ksantin ve L-Arginin seviyelerinin oosit maturasyonu, fertilizasyon ve gebelik sonuçları ile ilişkisi istatiksel olarak değerlendirilmiştir.

Bulgular: Matür oosit ve fertilize olan oosit sayıları ile serumdan ve folikülden bakılan Koenzim Q10, Myoinositol, Asta ksantin ve L-arginin değerleri arasında korelasyon saptanmamıştır( p<0.05). Toplam gonadotropin dozları ile toplanan oosit, matür oosit ve fertilize oosit sayıları arasında negatif koralasyon izlenmiştir ( p<0.05). Folliküler fazın 3. Günü alınan serumda, oosit toplama günü serumda ve folikülden bakılan Koenzim Q1O, Myoinositol ve L-arginin değerleri gebe kalan ve gebe kalmayanlar arasında anlamlı bir farklılık saptanmamıştır ( p<0.05). Folliküler fazın 3. Günü serumda bakılan Asta ksantin değeri ≤ 0,3035 olduğunda gebelik olasılığı daha yüksek bulunmuştur. Folliküler fazın 3. Günü alınan serumda, oosit toplama günü serumda ve folikülden bakılan Koenzim Q1O, Asta ksantin ve L-arginin değerlerinde klinik gebelik ve biyokimyasal gebelik geçirenler arasında anlamlı bir farklılık saptanmamıştır ( p<0.05). Oosit toplama günü serumda bakılan Myoinositol değeri ≥ 1,6555 olduğunda biyokimyasal gebelik olasılığı daha yüksek bulunmuştur.

Sonuç: Araştırma hipotezimizin daha güçlü sonuçları için daha geniş hasta popülasyonları
ile çalışmalara ihtiyaç vardır.

Anahtar kelimeler: Folikül sıvısı, Serum, İnfertilite, İVF, Koenzim Q10, Myoinositol, Asta
Ksantin, L-Arginin

Project Number

Harran Üniversitesi Bilimsel Araştırma Projeler Koordinatörlüğü (HÜBAK) 22079 nolu projesi

References

  • 1. Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 2005 Jul 14;3:28.
  • 2. Pierce JD, Cackler AB, Arnett MG. Why should you care about free radicals? RN. 2004;67:38–42. quiz 43.
  • 3. Malamitsi-Puchner A, Sarandakou A, Baka S. Concentrations ofangiogenic factors in follicular fluid and oocyte-cumulus complex culture medium from women undergoing in vitro fertilization. Association with oocyte maturity and fertiliza-tion. Fertil Steril 2001;76:98–101.
  • 4. Onalan G, Selam B, Baran Y. Serum and follicular fluid levels of soluble Fas, soluble Fas ligand and apoptosis of luteinized granulosa cells in PCOS patients undergoing IVF. Hum Reprod 2005;20(9):2391–5
  • 5. Guerin P, El Mouatassim S, Menezo Y. Oxidative stress and protection against reactive oxygen species in the pre-implantation embriyo and its surroundings. Hum Reprod Up-date 2001;7:175-89.
  • 6. Sarandakou A, Malamitsi-Puchner A, Baka S, Rizos D, Hassia-kos D, Creatsas G. Apoptosis and proliferation factors in se-rum and follicular fluid from women undergoing in vitro ferti-lization. Fertil Steril 2003;79:634–6
  • 7. Al-Saleh I, Coskun S, Al-Rouqi R, Al-Rajudi T, Eltabache C, Abduljabbar M, Al-Hassan S. Oxidative stress and DNA dama-ge status in couples undergoing in vitro fertilization treat-ment. Reprod Fertil. 2021 May 18;2(2):117-139. doi: 10.1530/RAF-20-0062.
  • 8. Showell MG, Mackenzie-Proctor R, Jordan V, Hart RJ. Anti-oxidants for female subfertility. Cochrane Database Syst Rev. 2020 Aug 27;8(8):CD007807. doi: 10.1002/14651858.CD007807.pub4.
  • 9. Baker VL, Luke B, Brown MB, Alvero R, Frattarelli JL, Usadi R, Grainger DA, Armstrong AY. Multivariate analysis of factors affecting probability of pregnancy and live birth with in vitro fertilization: an analysis of the Society for Assisted Reproduc-tive Technology Clinic Outcomes Reporting System. Fertil Ste-ril. 2010 Sep;94(4):1410-1416. doi: 10.1016/j.fertnstert.2009.07.986. Epub 2009 Sep 9. PMID: 19740463.
  • 10. Romero S, Pella R, Zorrilla I, Berrío P, Escudero F, Pérez Y, García M, Gonzalez C, Orihuela P. Coenzyme Q10 improves the in vitro maturation of oocytes exposed to the intrafollicu-lar environment of patients on fertility treatment. JBRA Assist Reprod. 2020 Jul 14;24(3):283-288.
  • 11. Turi A, Giannubilo SR, Brugè F, Principi F, Battistoni S, Santoni F, Tranquilli AL, Littarru G, Tiano L. Coenzyme Q10 content in follicular fluid and its relationship with oocyte fertilization and embryo grading. Arch Gynecol Obstet. 2012 Apr;285(4):1173-6.
  • 12. Akarsu S, Gode F, Isik AZ, Dikmen ZG, Tekindal MA. The asso-ciation between coenzyme Q10 concentrations in follicular fluid with embryo morphokinetics and pregnancy rate in as-sisted reproductive techniques. J Assist Reprod Genet. 2017 May;34(5):599-605. doi: 10.1007/s10815-017-0882-x. Epub 2017 Feb 9. Erratum in: J Assist Reprod Genet. 2017 May;34(5):607.
  • 13. Boots CE, Boudoures A, Zhang W, Drury A, Moley KH. Obe-sity-induced oocyte mitochondrial defects are partially pre-vented and rescued by supplementation with co-enzyme Q10 in a mouse model. Hum Reprod. 2016 Sep;31(9):2090-7. doi: 10.1093/humrep/dew181. Epub 2016 Jul 17.
  • 14. Alaa Abdulateef Mohammed , Zainab Hassan Al-Khafajy. Ass. Prof Dr. Wasan Adnan Abdullhameed. Coenzyme Q10 in the Follicular Fluid and Its Relation to Oocyte Maturity, Fertiliza-tion Rate, Embryo Grading, and Pregnancy Rate. Indian Jour-nal of Forensic Medicine & Toxicology, January-March 2021, Vol. 15, No. 1 753
  • 15. Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in hu-man fluids. J Clin Pathol. 2001 May;54(5):356-61.
  • 16. Chiu TT, Rogers MS, Law EL, Briton-Jones CM, Cheung LP, Haines CJ. Follicular fluid and serum concentrations of myo-inositol in patients undergoing IVF: relationship with oocyte quality. Hum Reprod. 2002 Jun;17(6):1591-6.
  • 17. Downes CP. Twenty-fifth Colworth medal lecture. The cellu-lar functions of myo-inositol. Biochem Soc Trans. 1989 Apr;17(2):259-68.
  • 18. Gharaei R, Alyasin A, Mahdavinezhad F, Samadian E, Ashraf-nezhad Z, Amidi F. Randomized controlled trial of astaxanthin impacts on antioxidant status and assisted reproductive technology outcomes in women with polycystic ovarian synd-rome. J Assist Reprod Genet. 2022 Apr;39(4):995-1008.
  • 19. Chelenga M, Sakaguchi K, Kawano K, Furukawa E, Yanagawa Y, Katagiri S, Nagano M. Low oxygen environment and as-taxanthin supplementation promote the developmental competence of bovine oocytes derived from early antral fol-licles during 8 days of in vitro growth in a gas-permeable cul-ture device. Theriogenology. 2022 Jan 1;177:116-126.
  • 20. Battaglia C, Regnani G, Marsella T, Facchinetti F, Volpe A, Venturoli S, Flamigni C. Adjuvant L-arginine treatment in controlled ovarian hyperstimulation: a double-blind, rando-mized study. Hum Reprod. 2002 Mar;17(3):659-65.
  • 21. Bodis J, Farkas B, Nagy B, Kovacs K, Sulyok E. The Role of L-Arginine-NO System in Female Reproduction: A Narrative Re-view. Int J Mol Sci. 2022 Nov 28;23(23):14908.
  • 22. Aydogan Mathyk B, Aslan Cetin B, Vardagli D, Zengin E, Sofi-yeva N, Irez T, Ocal P. Comparison of antagonist mild and long agonist protocols in terms of follicular fluid total antioxidant capacity. Taiwan J Obstet Gynecol. 2018;57(2):194-199,
There are 22 citations in total.

Details

Primary Language Turkish
Subjects Clinical Sciences
Journal Section Research Article
Authors

Yusuf Ziya Kızıldemir 0000-0003-0094-8275

Sibel Sak 0000-0001-7401-3945

İsmail Koyuncu 0000-0002-9469-4757

Hacer Uyanıkoğlu This is me 0000-0003-0316-4900

Mert Ulaş Barut 0000-0002-7008-8218

Muhammet Erdal Sak 0000-0002-6570-1057

Project Number Harran Üniversitesi Bilimsel Araştırma Projeler Koordinatörlüğü (HÜBAK) 22079 nolu projesi
Early Pub Date April 26, 2023
Publication Date April 27, 2023
Submission Date February 23, 2023
Acceptance Date April 9, 2023
Published in Issue Year 2023 Volume: 20 Issue: 1

Cite

Vancouver Kızıldemir YZ, Sak S, Koyuncu İ, Uyanıkoğlu H, Barut MU, Sak ME. Serum ve Foliküler Sıvıdaki Koenzim Q10, Myoinositol, Asta Ksantin ve L-Arginin Değerlerinin Icsı+Et ve Gebelik Sonuçları ile İlişkisi. Harran Üniversitesi Tıp Fakültesi Dergisi. 2023;20(1):208-17.

Harran Üniversitesi Tıp Fakültesi Dergisi  / Journal of Harran University Medical Faculty