Research Article
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Investigation of O-Alkyl/O-Alkenyl Glycerolipid Ratios in Erythrocyte Lysates of Patients with Obstructive Sleep Apnea

Year 2022, Volume 13, Issue 3, 309 - 315, 01.07.2022
https://doi.org/10.31067/acusaglik.1036563

Abstract

ABSTRACT Background: Vulnerabilities of plasmalogens in erythrocyte membrane to hypoxic conditions and alteration induced by sleep restriction make them potential biomarkers for the evaluation of sleep apnea related sleep disorders. Objective:We aimed to investigate and to compare ratios of alkyl/alkenyl glycerolipids of erythrocyte membranes (representing plasmanyl/plasmenyl plasmalogens) between controls and patients with sleep apnea and to evaluate possible differences, if any, which could be considered as a diagnostic tool. Material and methods: The patients underwent polysomnography and categorized according to the severity of sleep apnea using Apnea-Hypopnea Index (AHI). Phospholipids in erythrocyte lysates were hydrolyzed by phospholipase C and the acylgroups were saponified. Then the O-alkyl/O-alkenyl glycerolipids were separated by thin-layer chromatography (TLC). Densitometric image analyses were performed on the lipid spots of TLC plates and the ratios were determined. Results: No significant correlationwas observedbetween the alkyl/alkenyl glycerolipid ratios of the eryhtrocyte lysates of sleep apnea patients and that of controls. Conclusion: Results of this study warrants reinvestigation of alkyl/alkenyl glycerolipid ratios in erythrocytes of patients with sleep apnea, with each subjects sampled before and after the treatment of sleep apnea to better understand the potential of this ratio as a diagnostic tool.

References

  • Zhou L, Chen P, Peng Y, et al. Role of oxidative stress in the neurocognitive dysfunction of obstructive sleep apnea syndrome. Oxid Med Cell Longev. 2016;(2016):15.
  • Jaspers T, Morrell M, Simonds A, et al. The role of hypoxia and the circadian rhythm in sleep apnea. Eur. Respir. J. 2015;46 (issue suppl 59):OA298.
  • Davies SK, Ang JE, Revell VL, et al. Effect of sleep deprivation on the human metabolome. Proc Natl Acad Sci USA. 2014;111(29):10761-6.
  • Weljie AM, Meerlo P, Goel N, et al. Oxalic acid and diacylglycerol 36:3 are cross-species markers of sleep debt. PNAS. 2015;112(8):2569-74.
  • Chua ECP, Shui G, Cazenave-Gassiot A, et al. Changes in plasma lipids during exposure to total sleep deprivation. Sleep. 2015;38(11):1683-91.
  • Lee TC. 1998. Biosynthesis and possible biological functions of plasmalogens. Biochim Biophys Acta. 1394(2-3):129-145.
  • Watschinger W, Werner ER. Orphan enzymes in ether lipid metabolism. Biochimie. 2013; 95(1): 59-65.
  • Ford DA. Biosynthesis of plasmalogens in mammalian cells and their accelerated catabolism during cellular activation. In:Gross RW, editor. Advances in Lipobiology, USA: JAI Press, 1996: (1):176.
  • Su XQ, Wang J, Sinclair AJ. Plasmalogens and Alzheimer’s disease: a review. Lipids in Health and Disease. (2019) 18:100.
  • Berry RB, Budhiraja R, Gottlieb DJ, et al. Rules for scoring respiratory events in sleep: update of the 2007 AASM manual for the scoring of sleep and associated events. Deliberations of the sleep apnea definitions task force of the American Academy of Sleep Medicine. J Clin Sleep Med. 2012;8(5):597-619).
  • Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7.
  • Snyder F. Thin-layer chromatographic behavior of glycerolipid analogs containing ether, ester, hydroxyl, and ketone groupings. J. Chromatogr. 1973;82(I 973):7-14.
  • Senaratna CV, Perret JL, Lodge CL, et al. Prevalence of obstructive sleep apnea in thegeneral population: A systematic review. Sleep Med Rev. 2017;34: 70-81).
  • Stansbury RC, Strollo PJ. Clinical manifestations of sleep apnea. J Thorac Dis. 2015;7(9): E298-E310.
  • Snyder F. Ether lipids chemistry and biology. New York and London: Academic Press, 1972: 25-50.
  • Braverman NE, Moser AB. Functions of plasmalogen lipids in health and disease. BBA. 2012;1822(9):1442-52.
  • Maeba R, Nishimukai M, Sakasegawa SI, et al. Plasma/serum plasmalogens: methods of analysis and clinical significance. Advances in Clinical Chemistry, 1st ed. Elsevier Inc 2015: (70):31-94.
  • Diagne A, Fauvel J, Record M, et al. Studies on ether phospholipids II. comparative composition of various tissues from human, rat and guinea pig. BBA. 1984;793(2):221-31.
  • Connor WE, Lin DS, Thomas G, et al. Abnormal phospholipid molecular species of erythrocytes in sickle cell anemia. J Lipid Res. 1997;38:2516-28.
  • Moller-Levet CS, Archer SN, Bucca G, et al. Effects of insufficient sleep on circadian rhythmicity and expression amplitude of the human blood transcriptome. Proc Natl Acad Sci. 2013;110:E1132-E113241.
  • Haase VH. Hypoxic regulation of erythropoiesis and iron metabolism. Am J Physiol Renal Physiol. 2010;299(1):F1–13.
  • Huang W, Ramsey KM, Marcheva B, et al. Circadian rhythms, sleep, and metabolism. Neurobiology. 2011;121(6):2133-41.
  • Botto L, Beretta E, Bulbarelli A, et al. Hypoxia-induced modifications in plasma membranes and lipid microdomains in A549 cells and primary human alveolar cells. J Cell Biochem. 2008;105(2):503-13.
  • Maeba R, Maeda T, Kinoshita M, et al. Plasmalogens in human serum positively correlate with high- density lipoprotein and decrease with aging. J Atheroscler Thromb. 2007;Feb;14(1):12-8.
  • Labadaridis I, Moraitou M, Theodoraki M, et al. Plasmalogen levels in full-term neonates. Acta Paediatr. 2009;98(4):640-2.
  • Wang-Sattler R, Yu Y, Mittelstrass K, et al. Metabolic profiling reveals distinct variations linked to nicotine consumption in humans -first results from the kora study. PLoS ONE. 2008;3(12): e3863.
  • Osipenko AN. Change of plasmalogen content of red blood cells in myocardial hypoxia and acidosis. Acta Cardiologica. 2018;73(1):61-8.
  • Meikle PJ, Wong G, Barlow CK, et al. Plasma lipid profiling shows similar associations with prediabetes and Type 2 Diabetes. PLoS ONE. 2013:8(9): e74341.
  • Moser AB, Steinberg SJ, Watkins PA, et al. Human and great ape red blood cells differ in plasmalogen levels and composition. Lipids Health Dis. 2011;Jun 17;10:101.
  • Mawatari S, Katafuchi T, Miake K, et al. Dietary plasmalogen increases erythrocyte membrane plasmalogen in rats. Lipids Health Dis. 2012;11(1):161.

Year 2022, Volume 13, Issue 3, 309 - 315, 01.07.2022
https://doi.org/10.31067/acusaglik.1036563

Abstract

References

  • Zhou L, Chen P, Peng Y, et al. Role of oxidative stress in the neurocognitive dysfunction of obstructive sleep apnea syndrome. Oxid Med Cell Longev. 2016;(2016):15.
  • Jaspers T, Morrell M, Simonds A, et al. The role of hypoxia and the circadian rhythm in sleep apnea. Eur. Respir. J. 2015;46 (issue suppl 59):OA298.
  • Davies SK, Ang JE, Revell VL, et al. Effect of sleep deprivation on the human metabolome. Proc Natl Acad Sci USA. 2014;111(29):10761-6.
  • Weljie AM, Meerlo P, Goel N, et al. Oxalic acid and diacylglycerol 36:3 are cross-species markers of sleep debt. PNAS. 2015;112(8):2569-74.
  • Chua ECP, Shui G, Cazenave-Gassiot A, et al. Changes in plasma lipids during exposure to total sleep deprivation. Sleep. 2015;38(11):1683-91.
  • Lee TC. 1998. Biosynthesis and possible biological functions of plasmalogens. Biochim Biophys Acta. 1394(2-3):129-145.
  • Watschinger W, Werner ER. Orphan enzymes in ether lipid metabolism. Biochimie. 2013; 95(1): 59-65.
  • Ford DA. Biosynthesis of plasmalogens in mammalian cells and their accelerated catabolism during cellular activation. In:Gross RW, editor. Advances in Lipobiology, USA: JAI Press, 1996: (1):176.
  • Su XQ, Wang J, Sinclair AJ. Plasmalogens and Alzheimer’s disease: a review. Lipids in Health and Disease. (2019) 18:100.
  • Berry RB, Budhiraja R, Gottlieb DJ, et al. Rules for scoring respiratory events in sleep: update of the 2007 AASM manual for the scoring of sleep and associated events. Deliberations of the sleep apnea definitions task force of the American Academy of Sleep Medicine. J Clin Sleep Med. 2012;8(5):597-619).
  • Bligh EG, Dyer WJ. A rapid method of total lipid extraction and purification. Can J Biochem Physiol. 1959; 37(8):911-7.
  • Snyder F. Thin-layer chromatographic behavior of glycerolipid analogs containing ether, ester, hydroxyl, and ketone groupings. J. Chromatogr. 1973;82(I 973):7-14.
  • Senaratna CV, Perret JL, Lodge CL, et al. Prevalence of obstructive sleep apnea in thegeneral population: A systematic review. Sleep Med Rev. 2017;34: 70-81).
  • Stansbury RC, Strollo PJ. Clinical manifestations of sleep apnea. J Thorac Dis. 2015;7(9): E298-E310.
  • Snyder F. Ether lipids chemistry and biology. New York and London: Academic Press, 1972: 25-50.
  • Braverman NE, Moser AB. Functions of plasmalogen lipids in health and disease. BBA. 2012;1822(9):1442-52.
  • Maeba R, Nishimukai M, Sakasegawa SI, et al. Plasma/serum plasmalogens: methods of analysis and clinical significance. Advances in Clinical Chemistry, 1st ed. Elsevier Inc 2015: (70):31-94.
  • Diagne A, Fauvel J, Record M, et al. Studies on ether phospholipids II. comparative composition of various tissues from human, rat and guinea pig. BBA. 1984;793(2):221-31.
  • Connor WE, Lin DS, Thomas G, et al. Abnormal phospholipid molecular species of erythrocytes in sickle cell anemia. J Lipid Res. 1997;38:2516-28.
  • Moller-Levet CS, Archer SN, Bucca G, et al. Effects of insufficient sleep on circadian rhythmicity and expression amplitude of the human blood transcriptome. Proc Natl Acad Sci. 2013;110:E1132-E113241.
  • Haase VH. Hypoxic regulation of erythropoiesis and iron metabolism. Am J Physiol Renal Physiol. 2010;299(1):F1–13.
  • Huang W, Ramsey KM, Marcheva B, et al. Circadian rhythms, sleep, and metabolism. Neurobiology. 2011;121(6):2133-41.
  • Botto L, Beretta E, Bulbarelli A, et al. Hypoxia-induced modifications in plasma membranes and lipid microdomains in A549 cells and primary human alveolar cells. J Cell Biochem. 2008;105(2):503-13.
  • Maeba R, Maeda T, Kinoshita M, et al. Plasmalogens in human serum positively correlate with high- density lipoprotein and decrease with aging. J Atheroscler Thromb. 2007;Feb;14(1):12-8.
  • Labadaridis I, Moraitou M, Theodoraki M, et al. Plasmalogen levels in full-term neonates. Acta Paediatr. 2009;98(4):640-2.
  • Wang-Sattler R, Yu Y, Mittelstrass K, et al. Metabolic profiling reveals distinct variations linked to nicotine consumption in humans -first results from the kora study. PLoS ONE. 2008;3(12): e3863.
  • Osipenko AN. Change of plasmalogen content of red blood cells in myocardial hypoxia and acidosis. Acta Cardiologica. 2018;73(1):61-8.
  • Meikle PJ, Wong G, Barlow CK, et al. Plasma lipid profiling shows similar associations with prediabetes and Type 2 Diabetes. PLoS ONE. 2013:8(9): e74341.
  • Moser AB, Steinberg SJ, Watkins PA, et al. Human and great ape red blood cells differ in plasmalogen levels and composition. Lipids Health Dis. 2011;Jun 17;10:101.
  • Mawatari S, Katafuchi T, Miake K, et al. Dietary plasmalogen increases erythrocyte membrane plasmalogen in rats. Lipids Health Dis. 2012;11(1):161.

Details

Primary Language English
Subjects Biochemistry and Molecular Biology
Journal Section Research Article
Authors

Emine KOÇ>
INONU UNIVERSITY
0000-0003-3122-7104
Türkiye


Hilal ERMİŞ This is me
INONU UNIVERSITY
0000-0002-1764-9098
Türkiye


Harika GÖZÜKARA BAĞ>
INONU UNIVERSITY
0000-0003-1208-4072
Türkiye


Muhammed DÜNDAR This is me
INONU UNIVERSITY
0000-0001-6509-2012
Türkiye


Tayfun GÜLDÜR> (Primary Author)
İNÖNÜ ÜNİVERSİTESİ, TIP FAKÜLTESİ
0000-0002-1623-2880
Türkiye

Supporting Institution İnönü University, Scientific Research Unit
Project Number TYL-2017-623
Publication Date July 1, 2022
Application Date December 24, 2021
Acceptance Date March 27, 2022
Published in Issue Year 2022, Volume 13, Issue 3

Cite

EndNote %0 Acıbadem Üniversitesi Sağlık Bilimleri Dergisi Investigation of O-Alkyl/O-Alkenyl Glycerolipid Ratios in Erythrocyte Lysates of Patients with Obstructive Sleep Apnea %A Emine Koç , Hilal Ermiş , Harika Gözükara Bağ , Muhammed Dündar , Tayfun Güldür %T Investigation of O-Alkyl/O-Alkenyl Glycerolipid Ratios in Erythrocyte Lysates of Patients with Obstructive Sleep Apnea %D 2022 %J Acıbadem Üniversitesi Sağlık Bilimleri Dergisi %P 1309-470X-1309-5994 %V 13 %N 3 %R doi: 10.31067/acusaglik.1036563 %U 10.31067/acusaglik.1036563