Investigation of O-Alkyl/O-Alkenyl Glycerolipid Ratios in Erythrocyte Lysates of Patients with Obstructive Sleep Apnea

Emine Koç; Hilal Ermiş; Harika Gözükara Bağ; Muhammed Dündar; Tayfun Güldür

doi:10.31067/acusaglik.1036563

EN

Investigation of O-Alkyl/O-Alkenyl Glycerolipid Ratios in Erythrocyte Lysates of Patients with Obstructive Sleep Apnea

Abstract

ABSTRACT Background: Vulnerabilities of plasmalogens in erythrocyte membrane to hypoxic conditions and alteration induced by sleep restriction make them potential biomarkers for the evaluation of sleep apnea related sleep disorders. Objective:We aimed to investigate and to compare ratios of alkyl/alkenyl glycerolipids of erythrocyte membranes (representing plasmanyl/plasmenyl plasmalogens) between controls and patients with sleep apnea and to evaluate possible differences, if any, which could be considered as a diagnostic tool. Material and methods: The patients underwent polysomnography and categorized according to the severity of sleep apnea using Apnea-Hypopnea Index (AHI). Phospholipids in erythrocyte lysates were hydrolyzed by phospholipase C and the acylgroups were saponified. Then the O-alkyl/O-alkenyl glycerolipids were separated by thin-layer chromatography (TLC). Densitometric image analyses were performed on the lipid spots of TLC plates and the ratios were determined. Results: No significant correlationwas observedbetween the alkyl/alkenyl glycerolipid ratios of the eryhtrocyte lysates of sleep apnea patients and that of controls. Conclusion: Results of this study warrants reinvestigation of alkyl/alkenyl glycerolipid ratios in erythrocytes of patients with sleep apnea, with each subjects sampled before and after the treatment of sleep apnea to better understand the potential of this ratio as a diagnostic tool.

Keywords

Supporting Institution

İnönü University, Scientific Research Unit

Project Number

TYL-2017-623

References

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Emine Koç
0000-0003-3122-7104
Türkiye

Hilal Ermiş
0000-0002-1764-9098
Türkiye

Harika Gözükara Bağ
0000-0003-1208-4072
Türkiye

Muhammed Dündar
0000-0001-6509-2012
Türkiye

Tayfun Güldür ^*
0000-0002-1623-2880
Türkiye

Publication Date

July 1, 2022

Submission Date

December 24, 2021

Acceptance Date

March 27, 2022

Published in Issue

Year 1970 Volume: 13 Number: 3

DOI

https://doi.org/10.31067/acusaglik.1036563

IZ

https://izlik.org/JA68DM75XR

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RIS / Bibtex

EndNote

Koç E, Ermiş H, Gözükara Bağ H, Dündar M, Güldür T (July 1, 2022) Investigation of O-Alkyl/O-Alkenyl Glycerolipid Ratios in Erythrocyte Lysates of Patients with Obstructive Sleep Apnea. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi 13 3 309–315.

Öz

Investigation of O-Alkyl/O-Alkenyl Glycerolipid Ratios in Erythrocyte Lysates of Patients with Obstructive Sleep Apnea

Abstract

Keywords

Supporting Institution

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References

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Publication Date

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Acceptance Date

Published in Issue

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IZ

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