Research Article
Effects of Vitamin C Supplementation on the Metabolic Abnormalities Associated with Diabetes Mellitus
Abstract
Eksojen antioksidan takviyesinin, diyabetin mikro ve makrovasküler komplikasyonlarına karşı faydalı olabileceği bilinmektedir. Bu çalışmada C vitamini desteğinin, diyabetik ratlarda, kronik hiperglisemiye bağlı olarak bozulan insülin sekresyonu, hiperlipidemi, oksidatif stres ve paraoksonaz-1 enzim (PON1) aktivitesi üzerine etkilerinin araştırılması amaçlanmıştır. Bu amaçla, yirmi dört adet Wistar albino cinsi rat C, DC ve DCC grupları olarak 3 gruba ayrıldı. DC ve DCC grubundaki ratlarda, tek doz streptozotosin enjeksiyonu ile diyabet oluşturuldu (45 mg/kg). C ve DC grubundaki ratlar standart rat yemi (C vitamini içermeyen) ile beslenirken, DCC grubundaki ratlar 200 mg/kg dozunda C vitamini ile desteklenmiş deneysel yemle beslendi. Elde edilen bulgulara göre, C grubundaki ratların sekiz haftalık deney süresinin sonunda diyabetik ratlara göre daha az yem ve su tükettiği görüldü (p <0,001). Bunun yanı sıra C grubundaki ratların toplam kolesterol ve trigliserit düzeylerinin diyabetik gruplara göre daha düşük, PON1 aktivite ve insülin değerlerinin ise daha yüksek olduğu saptandı (p <0.05). Bir diğer yandan, DC ve DCC grupları arasında toplam kolesterol, trigliserid, HDL kolesterol, LDL kolesterol ve insülin düzeyleri açısından anlamlı farklılık gözlenmese de, DCC grubunun TAS ve PON1 aktivite düzeylerinin DC grubuna göre anlamlı olarak daha yüksek olduğu belirlendi (p <0.05). Sonuç olarak, diyabet varlığında, C vitamini takviyesi PON1 aktivite düzeyini ve antioksidan kapasiteyi artırmada etkili olabilir.
Project Number
17L0241003
References
- 1. Asmat U, Abad K and Ismail K. Diabetes mellitus and oxidative stress—A concise review. Saudi Pharm J. 2016; 24:547-553. DOI: 10.1016/j.jsps.2015.03.013
- 2. Zhang B, Liu S, Zhang L, et al. Admission fasting blood glucose predicts 30-day poor outcome in patients hospitalized for COVID-19 pneumonia. Diabetes Obes Metab. 2020;22:1955-1957. DOI: 10.1111/dom.14132
- 3. Wang S, Ma P, Zhang S, et al. Fasting blood glucose at admission is an independent predictor for 28-day mortality in patients with COVID-19 without previous diagnosis of diabetes: a multi-centre retrospective study. Diabetologia. 2020;63:2102-2111. DOI: 10.1007/s00125-020-05209-1
- 4. Ashor AW, Werner AD, Lara J, et al. Effects of vitamin C supplementation on glycaemic control: a systematic review and meta-analysis of randomised controlled trials. Eur J Clın Nutr. 2017;71:1371-1380. DOI: 10.1038/ejcn.2017.24
- 5. Cengiz M and Cengiz S. Tip 2 diyabetli hastalarda C vitamini uygulamasının eritrosit glutatyon ve HbA1c düzeyleri üzerine etkisi. Cerrahpasa Med J. 2000;31:211-215.
- 6. Mason SA, Della Gatta PA, Snow RJ, et al. Ascorbic acid supplementation improves skeletal muscle oxidative stress and insulin sensitivity in people with type 2 diabetes: findings of a randomized controlled study. Free Radic Biol Med. 2016;93:227-238. DOI: 10.1016/j.freeradbiomed.2016.01.006
- 7. Steffner RJ, Wu L, Powers AC, et al. Ascorbic acid recycling by cultured β cells: effects of increased glucose metabolism. Free Radic Biol Med. 2004;37:1612-1621. DOI: 10.1016/j.freeradbiomed.2004.07.032
- 8. Kaneto H, Kajimoto Y, Miyagawa JI, et al. Beneficial effects of antioxidants in diabetes: possible protection of pancreatic beta-cells against glucose toxicity. Diabetes. 1999;48:2398-2406. DOI: 10.2337/diabetes.48.12.2398
- 9. Gillani SW, Sulaiman SAS, Abdul MIM, et al. Combined effect of metformin with ascorbic acid versus acetyl salicylic acid on diabetes-related cardiovascular complication; a 12-month single blind multicenter randomized control trial. Cardiovasc Diabetol. 2017;16:103. DOI: 10.1186/s12933-017-0584-9
- 10. Dai S and McNeill JH. Ascorbic acid supplementation prevents hyperlipidemia and improves myocardial performance in streptozotocin-diabetic rats. Diabetes Res Clin Pract. 1995;27:11-18. DOI: 10.1016/0168-8227(94)01013-P
- 11. Wilson R, Willis J, Gearry R, et al. Inadequate vitamin C status in prediabetes and type 2 diabetes mellitus: Associations with glycaemic control, obesity, and smoking. Nutrients. 2017;9:997. DOI: 10.3390/nu9090997
- 12. Harding AH, Wareham NJ, Bingham SA, et al. Plasma vitamin C level, fruit and vegetable consumption, and the risk of new-onset type 2 diabetes mellitus: the European prospective investigation of cancer–Norfolk prospective study. Arch Intern Med. 2008;168:1493-1499. DOI: 10.1001/archinte.168.14.1493
- 13. Roghani M and Baluchnejadmojarad T. Hypoglycemic and hypolipidemic effect and antioxidant activity of chronic epigallocatechin-gallate in streptozotocin-diabetic rats. Pathophysiology. 2010:17:55-59. DOI: 10.1016/j.pathophys.2009.07.004
- 14. Zafar M and Naqvi SNUH. Effects of STZ-Induced Diabetes on the Relative Weights of Kidney, Liver and Pancreas in Albino Rats: A Comparative Study. Int J Morphol. 2010;28:135-142. DOI: 10.4067/S0717-95022010000100019.
- 15.Samarghandian S, Borji A, Delkhosh MB, et al. Safranal treatment improves hyperglycemia, hyperlipidemia and oxidative stress in streptozotocin-induced diabetic rats. J Pharm Pharm. 2013;16:352-362. DOI: 10.18433/j3zs3q
- 16. Florence NT, Benoit MZ, Jonas K, et al. Antidiabetic and antioxidant effects of Annona muricata (Annonaceae), aqueous extract on streptozotocin-induced diabetic rats. J Ethnopharmacol. 2014;151:784-790. DOI: 10.1016/j.jep.2013.09.021
- 17. Afkhami-Ardekani M and Shojaoddiny-Ardekani A. Effect of vitamin C on blood glucose, serum lipids & serum insulin in type 2 diabetes patients. Indian J Med Res. 2007;126:471-474.
- 18. Siavash M and Amini M. Vitamin C may have similar beneficial effects to Gemfibrozil on serum high-density lipoprotein-cholesterol in type 2 diabetic patients. J Res Pharm Pract. 2014;3:77-82. DOI: 10.4103/2279-042X.141075
- 19. Bhatt JK, Thomas S and Nanjan MJ. Effect of oral supplementation of vitamin C on glycemic control and lipid profile in patients with type 2 diabetes mellitus. Int J Pharm Pharm. 2012;4:524-527.
- 20. Uslu H. Effect of alpha lipoic acid and vitamin C supplemantation on oxidative stress and lipid profile in diabetic rats induced with streptozotocin. Kafkas University Health Sciences Institute Philosophy Thesis. 2013.
- 21. Vuppalapati L, Velayudam R, Ahamed KN, et al. The protective effect of dietary flavonoid fraction from Acanthophora spicifera on streptozotocin induced oxidative stress in diabetic rats. Food Science and Human Wellness. 2016;5:57-64. DOI: 10.1016/j.fshw.2016.02.002
- 22. Hazman Ö and Ovalı S. Investigation of the anti-inflammatory effects of safranal on high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model. Inflammation. 2015;38:1012-1019. DOI: 10.1007/s10753-014-0065-1
- 23. Chao JCJ, Yuan MD, Chen PY, et al. Vitamin C and E supplements improve the impaired antioxidant status and decrease plasma lipid peroxides in hemodialysis patients. J Nutr Biochem. 2002;13:653-663. DOI: 10.1016/s0955-2863(02)00209-7
- 24. Santhrani T, Maheswari E and Saraswathy GR. Amelioration of carbamazepine induced oxidative stress and hematotoxicity by vitamin C. Spatula DD. 2012;2:173-180. DOI: 10.5455/spatula.20121003014326
- 25. Flekač M, Škrha J, Zidkova K, et al. Paraoxonase 1 gene polymorphisms and enzyme activities in diabetes mellitus. Physiol Res. 2008;57:717-726. DOI: 10.33549/physiolres.931285
- 26. Ferretti G, Bacchetti T, Busni D, et al. Protective effect of paraoxonase activity in high-density lipoproteins against erythrocyte membranes peroxidation: a comparison between healthy subjects and type 1 diabetic patients. J Clin Endocrinol Metab. 2004;89:2957-2962. DOI: 10.1210/jc.2003-031897
- 27. Tartan Z, Orhan G, Kasıkçioglu H, et al. The role of paraoxonase (PON) enzyme in the extent and severity of the coronary artery disease in type-2 diabetic patients. Heart Vessels. 2007;22:158-164. DOI: 10.1007/s00380-006-0957-6
- 28. Jarvik GP, Tsai NT, McKinstry LA, et al. Vitamin C and E intake is associated with increased paraoxonase activity. Arterioscler Thromb Vasc Biol. 2002;22:1329-1333. DOI: 10.1161/01.atv.0000027101.40323.3a
- 29. Ferretti G, Bacchetti T, Masciangelo S, et al. Lipid peroxidation in hemodialysis patients: effect of vitamin C supplementation. Clin Biochem. 2008;41:381-386. DOI: 10.1016/j.clinbiochem.2007.12.011
Year 2022,
Volume: 13 Issue: 4, 593 - 598, 01.10.2022
Abstract
Supporting Institution
Ankara Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü
Project Number
17L0241003
References
- 1. Asmat U, Abad K and Ismail K. Diabetes mellitus and oxidative stress—A concise review. Saudi Pharm J. 2016; 24:547-553. DOI: 10.1016/j.jsps.2015.03.013
- 2. Zhang B, Liu S, Zhang L, et al. Admission fasting blood glucose predicts 30-day poor outcome in patients hospitalized for COVID-19 pneumonia. Diabetes Obes Metab. 2020;22:1955-1957. DOI: 10.1111/dom.14132
- 3. Wang S, Ma P, Zhang S, et al. Fasting blood glucose at admission is an independent predictor for 28-day mortality in patients with COVID-19 without previous diagnosis of diabetes: a multi-centre retrospective study. Diabetologia. 2020;63:2102-2111. DOI: 10.1007/s00125-020-05209-1
- 4. Ashor AW, Werner AD, Lara J, et al. Effects of vitamin C supplementation on glycaemic control: a systematic review and meta-analysis of randomised controlled trials. Eur J Clın Nutr. 2017;71:1371-1380. DOI: 10.1038/ejcn.2017.24
- 5. Cengiz M and Cengiz S. Tip 2 diyabetli hastalarda C vitamini uygulamasının eritrosit glutatyon ve HbA1c düzeyleri üzerine etkisi. Cerrahpasa Med J. 2000;31:211-215.
- 6. Mason SA, Della Gatta PA, Snow RJ, et al. Ascorbic acid supplementation improves skeletal muscle oxidative stress and insulin sensitivity in people with type 2 diabetes: findings of a randomized controlled study. Free Radic Biol Med. 2016;93:227-238. DOI: 10.1016/j.freeradbiomed.2016.01.006
- 7. Steffner RJ, Wu L, Powers AC, et al. Ascorbic acid recycling by cultured β cells: effects of increased glucose metabolism. Free Radic Biol Med. 2004;37:1612-1621. DOI: 10.1016/j.freeradbiomed.2004.07.032
- 8. Kaneto H, Kajimoto Y, Miyagawa JI, et al. Beneficial effects of antioxidants in diabetes: possible protection of pancreatic beta-cells against glucose toxicity. Diabetes. 1999;48:2398-2406. DOI: 10.2337/diabetes.48.12.2398
- 9. Gillani SW, Sulaiman SAS, Abdul MIM, et al. Combined effect of metformin with ascorbic acid versus acetyl salicylic acid on diabetes-related cardiovascular complication; a 12-month single blind multicenter randomized control trial. Cardiovasc Diabetol. 2017;16:103. DOI: 10.1186/s12933-017-0584-9
- 10. Dai S and McNeill JH. Ascorbic acid supplementation prevents hyperlipidemia and improves myocardial performance in streptozotocin-diabetic rats. Diabetes Res Clin Pract. 1995;27:11-18. DOI: 10.1016/0168-8227(94)01013-P
- 11. Wilson R, Willis J, Gearry R, et al. Inadequate vitamin C status in prediabetes and type 2 diabetes mellitus: Associations with glycaemic control, obesity, and smoking. Nutrients. 2017;9:997. DOI: 10.3390/nu9090997
- 12. Harding AH, Wareham NJ, Bingham SA, et al. Plasma vitamin C level, fruit and vegetable consumption, and the risk of new-onset type 2 diabetes mellitus: the European prospective investigation of cancer–Norfolk prospective study. Arch Intern Med. 2008;168:1493-1499. DOI: 10.1001/archinte.168.14.1493
- 13. Roghani M and Baluchnejadmojarad T. Hypoglycemic and hypolipidemic effect and antioxidant activity of chronic epigallocatechin-gallate in streptozotocin-diabetic rats. Pathophysiology. 2010:17:55-59. DOI: 10.1016/j.pathophys.2009.07.004
- 14. Zafar M and Naqvi SNUH. Effects of STZ-Induced Diabetes on the Relative Weights of Kidney, Liver and Pancreas in Albino Rats: A Comparative Study. Int J Morphol. 2010;28:135-142. DOI: 10.4067/S0717-95022010000100019.
- 15.Samarghandian S, Borji A, Delkhosh MB, et al. Safranal treatment improves hyperglycemia, hyperlipidemia and oxidative stress in streptozotocin-induced diabetic rats. J Pharm Pharm. 2013;16:352-362. DOI: 10.18433/j3zs3q
- 16. Florence NT, Benoit MZ, Jonas K, et al. Antidiabetic and antioxidant effects of Annona muricata (Annonaceae), aqueous extract on streptozotocin-induced diabetic rats. J Ethnopharmacol. 2014;151:784-790. DOI: 10.1016/j.jep.2013.09.021
- 17. Afkhami-Ardekani M and Shojaoddiny-Ardekani A. Effect of vitamin C on blood glucose, serum lipids & serum insulin in type 2 diabetes patients. Indian J Med Res. 2007;126:471-474.
- 18. Siavash M and Amini M. Vitamin C may have similar beneficial effects to Gemfibrozil on serum high-density lipoprotein-cholesterol in type 2 diabetic patients. J Res Pharm Pract. 2014;3:77-82. DOI: 10.4103/2279-042X.141075
- 19. Bhatt JK, Thomas S and Nanjan MJ. Effect of oral supplementation of vitamin C on glycemic control and lipid profile in patients with type 2 diabetes mellitus. Int J Pharm Pharm. 2012;4:524-527.
- 20. Uslu H. Effect of alpha lipoic acid and vitamin C supplemantation on oxidative stress and lipid profile in diabetic rats induced with streptozotocin. Kafkas University Health Sciences Institute Philosophy Thesis. 2013.
- 21. Vuppalapati L, Velayudam R, Ahamed KN, et al. The protective effect of dietary flavonoid fraction from Acanthophora spicifera on streptozotocin induced oxidative stress in diabetic rats. Food Science and Human Wellness. 2016;5:57-64. DOI: 10.1016/j.fshw.2016.02.002
- 22. Hazman Ö and Ovalı S. Investigation of the anti-inflammatory effects of safranal on high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model. Inflammation. 2015;38:1012-1019. DOI: 10.1007/s10753-014-0065-1
- 23. Chao JCJ, Yuan MD, Chen PY, et al. Vitamin C and E supplements improve the impaired antioxidant status and decrease plasma lipid peroxides in hemodialysis patients. J Nutr Biochem. 2002;13:653-663. DOI: 10.1016/s0955-2863(02)00209-7
- 24. Santhrani T, Maheswari E and Saraswathy GR. Amelioration of carbamazepine induced oxidative stress and hematotoxicity by vitamin C. Spatula DD. 2012;2:173-180. DOI: 10.5455/spatula.20121003014326
- 25. Flekač M, Škrha J, Zidkova K, et al. Paraoxonase 1 gene polymorphisms and enzyme activities in diabetes mellitus. Physiol Res. 2008;57:717-726. DOI: 10.33549/physiolres.931285
- 26. Ferretti G, Bacchetti T, Busni D, et al. Protective effect of paraoxonase activity in high-density lipoproteins against erythrocyte membranes peroxidation: a comparison between healthy subjects and type 1 diabetic patients. J Clin Endocrinol Metab. 2004;89:2957-2962. DOI: 10.1210/jc.2003-031897
- 27. Tartan Z, Orhan G, Kasıkçioglu H, et al. The role of paraoxonase (PON) enzyme in the extent and severity of the coronary artery disease in type-2 diabetic patients. Heart Vessels. 2007;22:158-164. DOI: 10.1007/s00380-006-0957-6
- 28. Jarvik GP, Tsai NT, McKinstry LA, et al. Vitamin C and E intake is associated with increased paraoxonase activity. Arterioscler Thromb Vasc Biol. 2002;22:1329-1333. DOI: 10.1161/01.atv.0000027101.40323.3a
- 29. Ferretti G, Bacchetti T, Masciangelo S, et al. Lipid peroxidation in hemodialysis patients: effect of vitamin C supplementation. Clin Biochem. 2008;41:381-386. DOI: 10.1016/j.clinbiochem.2007.12.011
There are 29 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Nutrition and Dietetics, Endocrinology |
| Journal Section | Research Article |
| Authors | |
| Project Number | 17L0241003 |
| Publication Date | October 1, 2022 |
| Submission Date | October 3, 2021 |
| Published in Issue | Year 2022Volume: 13 Issue: 4 |
