β-hydroxybutyrate Does Not Influence Viability and Clonogenicity of A549 Lung Cancer Cells

Ali Burak Özkaya; Senanur Malcanlı; Oyku Gonul Geyik

doi:10.31067/acusaglik.1221155

EN

β-hydroxybutyrate Does Not Influence Viability and Clonogenicity of A549 Lung Cancer Cells

Abstract

Background/Purpose: The metabolic shift from catabolism of carbohydrates to lipids results in production of ketone bodies leading to a state called ketosis. Ketosis via ketone supplement or ketogenic diet has been proposed as a non-toxic therapeutic option for a broad range of malignancies. Although the clinical impact of ketogenic diet is well-documented, the effect of ketone bodies on cancer cell biology is not clear for some cancers including non-small-cell lung cancer (NSCLC). In this study, we aimed to demonstrate the effects of the most prominent ketone body, β-hydroxybutyrate, on a NSCLC cell line, A549. Methods: A549 cell line was utilized as the in vitro model in this study. The effects of different β-hydroxybutyrate concentrations on cell viability were measured via sulforhodamine-B (SRB) viability assay. Long term effects of ketosis were evaluated via colony formation assay. Finally, the effect of β-hydroxybutyrate on cell migration was determined via scratch assay. Results: Our results suggest that introduction of β-hydroxybutyrate in physiologically relevant concentrations into the cell culture media does not influence cell viability, clonogenicity or migration. Conclusion: β-hydroxybutyrate has been previously demonstrated to induce, inhibit or does not influence the viability of different cell lines but there is no report regarding its effects on NSCLC cells. Here we report that physiologically relevant concentrations of β-hydroxybutyrate have no effect on viability, clonogenicity and migration of A549 cells.

Keywords

References

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Details

Primary Language

English

Subjects

Biochemistry and Cell Biology (Other)

Journal Section

Research Article

Authors

Ali Burak Özkaya ^*
0000-0002-4811-2082
Türkiye

Senanur Malcanlı
0000-0003-3811-8146
Türkiye

Oyku Gonul Geyik
0000-0003-3014-1253
Türkiye

Publication Date

March 17, 2023

Submission Date

December 19, 2022

Acceptance Date

February 19, 2023

Published in Issue

Year 1970 Volume: 14 Number: 2

DOI

https://doi.org/10.31067/acusaglik.1221155

IZ

https://izlik.org/JA27LA76UF

Cite

RIS / Bibtex

EndNote

Özkaya AB, Malcanlı S, Gonul Geyik O (March 1, 2023) β-hydroxybutyrate Does Not Influence Viability and Clonogenicity of A549 Lung Cancer Cells. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi 14 2 97–102.