Research Article
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Antioxidant and Anti-inflammatory Activity of Five Centaurea Species

Year 2023, Volume: 82 Issue: 2, 311 - 316, 21.12.2023

Ali Şen

https://doi.org/10.26650/EurJBiol.2023.1340790

Abstract

Objective: In this study, we examined the anti-inflammatory and antioxidant (ABTS radical scavenging) activity of methanol extracts of aerial parts (except capitula) and capitula of Centaurea cuneifolia, C. iberica, C. kilaea, C. solstitialis subsp. solstitialis and C. stenolepis for the first time comparatively.

Materials and Methods: The antioxidant and anti-inflammatory activity, expressed as IC50 values, were determined by 2, 2’- Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic asit (ABTS) and 5-lipoxygenase methods. The total phenolic content, expressed as gallic acid equivalents, was estimated by Folin-Ciocalteu method.

Results: Methanol extract of capitula of C. solstitialis subsp. solstitialis (CSSC) with an IC50 value of 8.74 µg/mL showed antioxidant activity as strong as standard acarbose (4.41 µg/mL) against ABTS radicals. The IC50 values of ABTS radical scavenging activities of other extracts varied between 24.42 and 88.95 µg/mL. CSSC with an IC50 value of 122.10 µg/mL displayed moderate inhibitory activity against 5-lipoxygenase enzyme. The IC50 values of the antilipoxygenase activities of the other extracts were found to vary between 122.10 and 781.30 µg/mL. Also, the highest amount of total phenolic compounds was found in the CSSC (83.41 mg/g), while the lowest was found in methanol extract of aerial parts of C. solstitialis subsp. solstitialis (35.20 mg/g).

Conclusion: These results clearly indicate that CSSC has significant antioxidant and anti-inflammatory activity. As far as is known, this paper is the first comparative study on ABTS radical scavenging and lipoxygenase inhibitory activity of five different Centaurea species. It is also the first study on the antilipoxygenase activity of C. iberica and C. solstitialis subsp. solstitialis.

Keywords

Centaurea species antioxidant activity anti-inflammatory activity total phenolic content

References

  • Jurcackova Z, Ciglanova D, Mudronova D, et al. Astaxanthin extract from Haematococcus pluvialis and its fractions of astax-anthin mono-and diesters obtained by CCC show differential an-tioxidant and cytoprotective effects on naıve-mouse spleen cells. Antioxidants. 2023;12(6):1144. doi:10.3390/antiox12061144 google scholar
  • Khan MMR, Susmi TF, Miah M, Reza MA, Rahi MS. Morpholog-ical alteration and intracellular ROS generation confirm apoptosis induction on EAC cells by Leucas indica bark extract. J Herbs Spices Med Plants. 2023;29(1):84-97. google scholar
  • Duh PD, Tu YY, Yen GC. Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat). LWT-Food Sci Technol. 1999;32(5):269-277. google scholar
  • Peake JM, Suzuki K, Coombes JS. The influence of antioxidant supplementation on markers of inflammation and the relationship to oxidative stress after exercise. J Nutr Biochem. 2007;18(6):357-371. google scholar
  • Kim NY, Kim S, Park HM, et al. Cinnamomum verum extract inhibits NOX2/ROS and PKCö/JNK/AP-1/NF-kB pathway-mediated inflammatory response in PMA-stimulated THP-1 monocytes. Phytomedicine. 2023;112:154685. doi:10.1016/j.phymed.2023.154685 google scholar
  • Chinsamy M, Finnie JF, Van Staden J. Anti-inflammatory, an-tioxidant, anti-cholinesterase activity and mutagenicity of South African medicinal orchids. SAfrJBot. 2014;91:88-98. google scholar
  • Bizim Bitkiler. https://bizimbitkiler.org.tr/v2/hiyerarsi.php?c= Centaurea . Accessed June 24, 2023. google scholar
  • Şen A, Bitiş L, Birteksöz-Tan S, Bulut G. In vitro evaluation of antioxidant and antimicrobial activities of some Centaurea L. species. Marmara Pharm J. 2013;17(1):42-45. google scholar
  • Sen A, Ozbas Turan S, Bitis L. Bioactivity-guided isolation of anti-proliferative compounds from endemic Centaurea kilaea. Pharm Biol. 2017;55(1):541-546. google scholar
  • Reyhan A, Küpeli E, Ergun F. The biological activity of Centaurea L. species. Gazi Univ J Sci. 2004;17(4):149-164. google scholar
  • Zou Y, Chang SKC, GuY, Qian SY. Antioxidant activity and phe-nolic compositions of Lentil (Lens culinaris var. morton) extract and its fractions. J Agric Food Chem. 2011;59(6):2268-2276. google scholar
  • Phosrithong N, Nuchtavorn N. Antioxidant and anti-inflammatory activites of Clerodendrum leaf extracts collected in Thailand. Eur J Integr Med. 2016;8(3):281-285. google scholar
  • Yıldırım A, Şen A, Doğan A, Bitis L. Antioxidant and anti-inflammatory activity of capitula, leaf and stem extracts of Tanacetum cilicicum (Boiss.) Grierson. Int J Second Metab. 2019;6(2):211-222. google scholar
  • Gao X, Ohlander M, Jeppsson N, Björk L, Trajkovski V. Changes in antioxidant effects and their relationship to phytonutrients in fruits of Sea buckthorn (Hippophae rhamnoides L.) during Mat-uration. J Agric Food Chem. 2000;48:1485-1490. google scholar
  • Aleksandrova K, Koelman L, Rodrigues CE. Dietary patterns and biomarkers of oxidative stress and inflammation: A system-atic review of observational and intervention studies. Redox Biol. 2021;42:101869. doi:10.1016/j.redox.2021.101869 google scholar
  • Hassan W, Noreen H, Rehman S, et al. Oxidative stress and an-tioxidant potential of one hundred medicinal plants. Curr Top Med Chem. 2017;17(12):1336-1370. google scholar
  • Krishnaiah D, Sarbatly R, Nithyanandam, R. A review of the antioxidant potential of medicinal plant species. Food Bioprod Process. 2011;89(3):217-233. google scholar
  • Oguntibeju OO. Medicinal plants with anti-inflammatory activi-ties from selected countries and regions of Africa. J Inflamm Res. 2018;11:307-317. google scholar
  • Necip A, Durgun M. Antioxidant properties, total phenolic content and LC-MS/MS analysis of Mentha pulegium, Lep-idium draba and Centaurea solstitialis. J Inst Sci Technol. 2022;12(4):2375-2385. google scholar
  • Sekerler T, Sen A, Bitis L, Sener A. Anticancer, antioxidant and anti-inflammatory activities of chloroform extracts from some Centaurea species. MDPI-Proceedings. 2018;2(25):1542. doi:10.3390/proceedings2251542 google scholar
  • Alper M, Özay C, Güneş H, Mammadov R. Assessment of an-tioxidant and cytotoxic activities and identification of pheno-lic compounds of Centaurea solstitialis and Urospermum pi-croides from Turkey. Braz Arch Biol Technol. 2021;64:e21190530. doi:10.1590/1678-4324-2021190530 google scholar
  • Akkol EK, Arif R, Ergun F, Yesilada E. Sesquiterpene lactones with antinociceptive and antipyretic activity from two Centaurea species. J Ethnopharmacol. 2009;122(2):210-215. google scholar
  • Croft KD. The chemistry and biological effects of flavonoids and phenolic acids. Ann N Y Acad Sci. 1998;854(1):435-442. google scholar
  • Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant ac-tivity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996;20(7):933-956. google scholar
  • Kumar N, Goel N. Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnol Rep. 2019;24:e00370. doi:10.1016/j.btre.2019.e00370 google scholar
  • Gonzalez R, Ballester I, Lopez-Posadas R, et al. Effects of flavonoids and other polyphenols on inflammation. Crit Rev Food Sci Nutr. 2011;51(4):331-362. google scholar
  • Matos MS, Anastacio JD, Nunes dos Santos C. Sesquiterpene lactones: Promising natural compounds to fight inflammation. Pharmaceutics. 2021;13(7):991. google scholar

Year 2023, Volume: 82 Issue: 2, 311 - 316, 21.12.2023

Ali Şen

https://doi.org/10.26650/EurJBiol.2023.1340790

Abstract

References

  • Jurcackova Z, Ciglanova D, Mudronova D, et al. Astaxanthin extract from Haematococcus pluvialis and its fractions of astax-anthin mono-and diesters obtained by CCC show differential an-tioxidant and cytoprotective effects on naıve-mouse spleen cells. Antioxidants. 2023;12(6):1144. doi:10.3390/antiox12061144 google scholar
  • Khan MMR, Susmi TF, Miah M, Reza MA, Rahi MS. Morpholog-ical alteration and intracellular ROS generation confirm apoptosis induction on EAC cells by Leucas indica bark extract. J Herbs Spices Med Plants. 2023;29(1):84-97. google scholar
  • Duh PD, Tu YY, Yen GC. Antioxidant activity of water extract of Harng Jyur (Chrysanthemum morifolium Ramat). LWT-Food Sci Technol. 1999;32(5):269-277. google scholar
  • Peake JM, Suzuki K, Coombes JS. The influence of antioxidant supplementation on markers of inflammation and the relationship to oxidative stress after exercise. J Nutr Biochem. 2007;18(6):357-371. google scholar
  • Kim NY, Kim S, Park HM, et al. Cinnamomum verum extract inhibits NOX2/ROS and PKCö/JNK/AP-1/NF-kB pathway-mediated inflammatory response in PMA-stimulated THP-1 monocytes. Phytomedicine. 2023;112:154685. doi:10.1016/j.phymed.2023.154685 google scholar
  • Chinsamy M, Finnie JF, Van Staden J. Anti-inflammatory, an-tioxidant, anti-cholinesterase activity and mutagenicity of South African medicinal orchids. SAfrJBot. 2014;91:88-98. google scholar
  • Bizim Bitkiler. https://bizimbitkiler.org.tr/v2/hiyerarsi.php?c= Centaurea . Accessed June 24, 2023. google scholar
  • Şen A, Bitiş L, Birteksöz-Tan S, Bulut G. In vitro evaluation of antioxidant and antimicrobial activities of some Centaurea L. species. Marmara Pharm J. 2013;17(1):42-45. google scholar
  • Sen A, Ozbas Turan S, Bitis L. Bioactivity-guided isolation of anti-proliferative compounds from endemic Centaurea kilaea. Pharm Biol. 2017;55(1):541-546. google scholar
  • Reyhan A, Küpeli E, Ergun F. The biological activity of Centaurea L. species. Gazi Univ J Sci. 2004;17(4):149-164. google scholar
  • Zou Y, Chang SKC, GuY, Qian SY. Antioxidant activity and phe-nolic compositions of Lentil (Lens culinaris var. morton) extract and its fractions. J Agric Food Chem. 2011;59(6):2268-2276. google scholar
  • Phosrithong N, Nuchtavorn N. Antioxidant and anti-inflammatory activites of Clerodendrum leaf extracts collected in Thailand. Eur J Integr Med. 2016;8(3):281-285. google scholar
  • Yıldırım A, Şen A, Doğan A, Bitis L. Antioxidant and anti-inflammatory activity of capitula, leaf and stem extracts of Tanacetum cilicicum (Boiss.) Grierson. Int J Second Metab. 2019;6(2):211-222. google scholar
  • Gao X, Ohlander M, Jeppsson N, Björk L, Trajkovski V. Changes in antioxidant effects and their relationship to phytonutrients in fruits of Sea buckthorn (Hippophae rhamnoides L.) during Mat-uration. J Agric Food Chem. 2000;48:1485-1490. google scholar
  • Aleksandrova K, Koelman L, Rodrigues CE. Dietary patterns and biomarkers of oxidative stress and inflammation: A system-atic review of observational and intervention studies. Redox Biol. 2021;42:101869. doi:10.1016/j.redox.2021.101869 google scholar
  • Hassan W, Noreen H, Rehman S, et al. Oxidative stress and an-tioxidant potential of one hundred medicinal plants. Curr Top Med Chem. 2017;17(12):1336-1370. google scholar
  • Krishnaiah D, Sarbatly R, Nithyanandam, R. A review of the antioxidant potential of medicinal plant species. Food Bioprod Process. 2011;89(3):217-233. google scholar
  • Oguntibeju OO. Medicinal plants with anti-inflammatory activi-ties from selected countries and regions of Africa. J Inflamm Res. 2018;11:307-317. google scholar
  • Necip A, Durgun M. Antioxidant properties, total phenolic content and LC-MS/MS analysis of Mentha pulegium, Lep-idium draba and Centaurea solstitialis. J Inst Sci Technol. 2022;12(4):2375-2385. google scholar
  • Sekerler T, Sen A, Bitis L, Sener A. Anticancer, antioxidant and anti-inflammatory activities of chloroform extracts from some Centaurea species. MDPI-Proceedings. 2018;2(25):1542. doi:10.3390/proceedings2251542 google scholar
  • Alper M, Özay C, Güneş H, Mammadov R. Assessment of an-tioxidant and cytotoxic activities and identification of pheno-lic compounds of Centaurea solstitialis and Urospermum pi-croides from Turkey. Braz Arch Biol Technol. 2021;64:e21190530. doi:10.1590/1678-4324-2021190530 google scholar
  • Akkol EK, Arif R, Ergun F, Yesilada E. Sesquiterpene lactones with antinociceptive and antipyretic activity from two Centaurea species. J Ethnopharmacol. 2009;122(2):210-215. google scholar
  • Croft KD. The chemistry and biological effects of flavonoids and phenolic acids. Ann N Y Acad Sci. 1998;854(1):435-442. google scholar
  • Rice-Evans CA, Miller NJ, Paganga G. Structure-antioxidant ac-tivity relationships of flavonoids and phenolic acids. Free Radic Biol Med. 1996;20(7):933-956. google scholar
  • Kumar N, Goel N. Phenolic acids: Natural versatile molecules with promising therapeutic applications. Biotechnol Rep. 2019;24:e00370. doi:10.1016/j.btre.2019.e00370 google scholar
  • Gonzalez R, Ballester I, Lopez-Posadas R, et al. Effects of flavonoids and other polyphenols on inflammation. Crit Rev Food Sci Nutr. 2011;51(4):331-362. google scholar
  • Matos MS, Anastacio JD, Nunes dos Santos C. Sesquiterpene lactones: Promising natural compounds to fight inflammation. Pharmaceutics. 2021;13(7):991. google scholar
There are 27 citations in total.

Details

Primary Language English
Subjects Biochemistry and Cell Biology (Other)
Journal Section Themed Articles - Research Articles
Authors

Ali Şen 0000-0002-2144-5741

Publication Date December 21, 2023
Submission Date August 10, 2023
Published in Issue Year 2023 Volume: 82 Issue: 2

Cite

AMA Şen A. Antioxidant and Anti-inflammatory Activity of Five Centaurea Species. Eur J Biol. December 2023;82(2):311-316. doi:10.26650/EurJBiol.2023.1340790
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