Research Article
Year 2022,
Volume: 6 Issue: 3, 858 - 862, 29.09.2022
Abstract
Tescalcin (TESC) has been shown to be essential in mammalian cells as a regulator of intracellular Ca2+. Ca2+ is a second messenger functioning in many metabolic pathways as well as in cell differentiation, cell size and the cell cycle. K562 cells over-expressing TESC change their morphology and adopt adherent properties. Considering differences in morphology may have been reflected in changes of the cytoskeleton, we focused on the expression levels of keratins, which are cytoskeletal intermediate filaments in epithelial cells and also expressed in K562. We over-expressed the TESC gene via lentiviral transduction and analyzed keratin 8 (K8), keratin 18 (K18), and keratin 19 (K19) expression.
References
- 1. Gutierrez-Ford C., Levay K., Gomes A. V., et al. Characterization of tescalcin, a novel EF-hand protein with a single Ca2+-binding site: metal-binding properties, localization in tissues and cells, and effect on calcineurin. Biochemistry 2003; 49; 14553–14565.
- 2. Mailänder J., Müller-Esterl W. and Dedio J. Human homolog of mouse tescalcin associates with Na(+)/H(+) exchanger type-1. FEBS Lett. 2001;507(3);331-335.
- 3. Okumura M., Yamakawa H., Ohara O. and Owaribe K. Novel alternative splicings of BPAG1 (bullous pemphigoid antigen 1) including the domain structure closely related to MACF (microtubule actin cross-linking factor). J Biol Chem. 2002. 277(8);6682-6687.
- 4. Ganani D, Malovitski K, Sarig O, Gat A, Sprecher E, Samuelov L. Epidermolysis bullosa simplex due to bi-allelic DST mutations: Case series and review of the literature. Pediatr Dermatol. 2021; 38(2):436-441.
- 5. Croce C. M. Oncogenes and Cancer. N Engl J Med 2008; 358;502-511.
- 6. Jӓrvinen M., Andersson L. C. and Virtanen I., K562 Erythroleukemia cells express cytokeratins 8, 18, and 19 and epithelial membrane antigen that disappear after induced differentiation. J of cellular physiology 1990; 143; 310-320.
- 7. Omary M. B., Intermediate filament proteins of digestive organs: physiology and pathophysiology. Am J Physiol Gastrointest Liver Physiol 2017; 312; G628-G634.
- 8. Kim H. J., Choi W. J. and Lee C. H. Phosphorylation and Reorganization of Keratin Networks: Implications for Carcinogenesis and Epithelial Mesenchymal Transition. Biomol Ther (Seoul) 2015; 23(4); 301-312.
- 9. Baykal-Köse S., Acikgoz E., Yavuz A. S. , Geyik O. G., Ateş H, Sezerman O. U., et al. Adaptive phenotypic modulations lead to therapy resistance in chronic myeloid leukemia cells. PLoS ONE. 2020;15(2).
Year 2022,
Volume: 6 Issue: 3, 858 - 862, 29.09.2022
Abstract
References
- 1. Gutierrez-Ford C., Levay K., Gomes A. V., et al. Characterization of tescalcin, a novel EF-hand protein with a single Ca2+-binding site: metal-binding properties, localization in tissues and cells, and effect on calcineurin. Biochemistry 2003; 49; 14553–14565.
- 2. Mailänder J., Müller-Esterl W. and Dedio J. Human homolog of mouse tescalcin associates with Na(+)/H(+) exchanger type-1. FEBS Lett. 2001;507(3);331-335.
- 3. Okumura M., Yamakawa H., Ohara O. and Owaribe K. Novel alternative splicings of BPAG1 (bullous pemphigoid antigen 1) including the domain structure closely related to MACF (microtubule actin cross-linking factor). J Biol Chem. 2002. 277(8);6682-6687.
- 4. Ganani D, Malovitski K, Sarig O, Gat A, Sprecher E, Samuelov L. Epidermolysis bullosa simplex due to bi-allelic DST mutations: Case series and review of the literature. Pediatr Dermatol. 2021; 38(2):436-441.
- 5. Croce C. M. Oncogenes and Cancer. N Engl J Med 2008; 358;502-511.
- 6. Jӓrvinen M., Andersson L. C. and Virtanen I., K562 Erythroleukemia cells express cytokeratins 8, 18, and 19 and epithelial membrane antigen that disappear after induced differentiation. J of cellular physiology 1990; 143; 310-320.
- 7. Omary M. B., Intermediate filament proteins of digestive organs: physiology and pathophysiology. Am J Physiol Gastrointest Liver Physiol 2017; 312; G628-G634.
- 8. Kim H. J., Choi W. J. and Lee C. H. Phosphorylation and Reorganization of Keratin Networks: Implications for Carcinogenesis and Epithelial Mesenchymal Transition. Biomol Ther (Seoul) 2015; 23(4); 301-312.
- 9. Baykal-Köse S., Acikgoz E., Yavuz A. S. , Geyik O. G., Ateş H, Sezerman O. U., et al. Adaptive phenotypic modulations lead to therapy resistance in chronic myeloid leukemia cells. PLoS ONE. 2020;15(2).
There are 9 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Health Care Administration |
| Journal Section | Research Article |
| Authors | |
| Publication Date | September 29, 2022 |
| Submission Date | April 12, 2022 |
| Published in Issue | Year 2022 Volume: 6 Issue: 3 |
