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Ratlarda Thermopsis turcica Bitkisinden Elde Edilen Ekstraktların Antioksidan Etkilerinin Araştırılması

Year 2016, Volume: 9 Issue: 4, 259 - 265, 01.12.2016

Abstract

Fabacea familyasına ait endemik bir bitki olan Thermopsis turcica ülkemizde Thermopsis cinsini temsil eden tek türdür. Yapılan çalışma ile Thermopsis turcica bitkisinden elde edilen ekstraktların antioksidan etkilerinin araştırılması amaçlandı. Çalışmada, 3- 4 aylık 250-350 gr ağırlığındaki Wistar Albino ırkı toplam 74 adet erkek rat kullanıldı. Ratlardan 64 tanesi antioksidan aktiviteyi, 10 tanesi ÖD50 dozu belirlemek için kullanıldı. Ratlar, her grupta 8 adet olacak şekilde 8 gruba ayrıldı. Thermopsis turcica bitkisinin toprak üstü kısımlarından hazırlanan ekstraktlar, belirlenen ÖD50 dozunun yaklaşık % 1’i, % 2’si ve % 4’ü oranlarında alınarak gruplandırmada belirlenen şekilde, günlük tek doz halinde eş zamanlı olarak gastrik gavaj yoluyla 30 gün boyunca uygulandı. Çalışma sonunda hayvanlardan, kan ve karaciğer, böbrek doku örnekleri alındı. Kan ve doku örneklerinden Malondialdehit (MDA), Redükte Glutatyon (GSH), Süperoksit Dismutaz (SOD), Katalaz (CAT), ve plazma Antioksidan Aktivite (AOA) değerlerine bakıldı. Çalışmada, Thermopsis turcica bitkisinin toprak üstü kısımlarından hazırlanan etanol ve su ekstraktlarının ratlara uygulanması sonucu öldürücü doz 50 (ÖD50) ˃ 2000 mg/kg olarak bulundu. Tam kan MDA seviyesinde görülen düşük değer ile tam kan ve doku örneklerinde belirlenen düşük GSH değerlerinin özellikle sulu ekstraktlarda görülmesi bitkinin antioksidan etki üzerine olumlu etkisinin olduğunu göstermektedir. Ancak, plazma AOA değerlerinin her iki ekstraktta da düşük bulunması yeterli bir antioksidan etki oluşmadığı kanaatine varmamızı sağlamıştır.

References

  • Aebi H. Catalase in vitro assay methods. Methods Enzymol. 1984; 105. 121-126.
  • Aksoy L, Kolay E, Ağılönü Y, Aslan Z, Kargıoğlu M. Free Radical Scavenging Activity, Total Antioxidant Status and Total Oxidant Status of Endemic Thermopsis turcica, Saudi Journal of Biological Science. 2013; 10: 1016.
  • Bayır Y. Usnea Longıssıma Ach. Liken Türünden İzole Edilen Difraktaik Asit’ in İndometazin Ülseri Üzerine Koruyucu Etkisi ve İn-Vivo Antioksidan Özelliklerinin Araştırılması. Yüksek Lisans Tezi, Atatürk Üniv. Sağlık Bilimleri Enstitüsü. 2004.
  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine. 1963; 61: 882-888.
  • Choudhary RK, Swarnkar PL. Antioxidant activity of phenolic and flavonoid compounds in some medicinal plants of india. Natural Product Research. 2011; 25 (11): 1101-1109.
  • Davis PH, Mill RR, Tan, Kıt. Flora of Turkey and the East Aegean Islands. University of Edinburg Press. Edinburg. 1988; 10: 112.
  • Dayan S. Endemik ve Tehlike Altındaki Thermopsis turcica (Fabaceae)’nın in vitro çimlenmesi ve mikroçogaltımı. Yüksek Lisans Tezi, Afyon Kocatepe Üniversitesi, Fen Bilimleri Enstitüsü. 2006.
  • Disilvestro RA, Goodman J, Dy E, Lavalle G. Soy isoflavone supplementation elevates erythrocyte superoxide dismutase, but not ceruloplasmin in postmenopausal breast cancer survivors. Breast Cancer Research and Treatment. 2005; 89: 251-255.
  • Draper HH, Hadley M. Malondialdehyde determination as index of lipid Peroxidation. Methods in Enzymology. 1990; 186: 421–431.
  • Dündar Y, Aslan R. Hekimlikte oksidatif stres ve antioksidanlar, Afyon Kocatepe Üniversitesi Yayınları. Afyon. 2000. Hu CC, Hsiao CH, Huang SY, Fu SH, Lai CC, Hong TM, Chen HH, Lu FJ. Antioxidant activity of fermented soybean extract. J Agric Food Chem. 2004; 52(18): 5735-9.
  • Ibrahim WH, Habib HM, Chow CK, Bruckner GG. Isoflavone-rich soy isolate reduces lipid peroxidation in mouse liver. Int J Vitam Nutr Res. 2008; 78(4-5): 217-22.
  • Khairunuur FA, Zulkhaırı A, Haıruszah I, Azrına A, Nursakınah I, Fazalı F, Kamal MNH, Zamree MS, Kamılah KAK. Hypolipemic and Weight Reducing Properties from Tamarindus indica L. Pulp Ekstract in Diet- İnduced Obese Rats. International Journal of Pharmacology. 2010; 6(3): 216-223. ISSN 1811-7775. Malaysia.
  • Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol. 2001; 54: 356-361.
  • Liu J, Chang SK, Wiesenborn D. Antioxidant properties of soybean isoflavone extract and tofu in vitro and in vivo. J Agric Food Chem. 2005; 53(6): 2333-40.
  • Luck H. Catalase. In: H.U. Bergmeyer (Eds.), Methods in Analysis, Academy Press, London. 1955.
  • Oecd. (2001). Guidelines for the Testing of Chemicals-425. [www.oecd.org/dataoecd, (02. 04. 2013)].
  • Okhawa Η, Onishi Ν, Yagi Κ. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 1979; 95: 351.
  • Raja S, Ahamed HN, Kumar V, Mukherje K, Bandyopadhyay A, Mukherje PK. Exploring the Effect of Cytisus Scoparius on Markers of Oxidative Stress in Rats. Iranıan Journal of Pharmacology & Therapeutıcs. 2007; 6: 15-21.
  • Rajaram K, Moushmi M, Velayutham MDP, Kumpati P, Ganasaraswathi M, Sureshkumar P. Comparative Bioactive Studies Between Wild Plant and Callus Culture of Tephrosia tinctoria Pers. Appl Biochem Biotechnol. 2013; 0444-3: 12010-013. DOI 10.1007.
  • Rajeshwar Y, Gupta M, Mazumder UK. Antitumor Activity and in vivo Antioxidant Status of Mucuna pruriens (Fabaceae) Seeds against Ehrlich Ascites Carcinoma in Swiss Albino Mice. Iranıan Journal of Pharmacology & Therapeutıcs. 2005; 4: 46-53.
  • Sinan B. Thermopsis turcica Kit Tan, Vural & Küçüködük (Fabaceae)’nın Morfolojisi, Anatomisi ve Ekolojisi. Yüksek Lisans Tezi. Selçuk Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı. 2002.
  • Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical Chemistry. 1988; 34: 497–500.
  • Şerbetçi H. Meyan (Glycyrrhiza glabra L.) Bitkisinin Antioksidan Kapasitesinin Belirlenmesi. Yüksek Lisans Tezi. Atatürk Üniversitesi Fen Bilimleri Enstitüsü. 2007.
  • Tezcan S. Thermopsis turcica (Fabaceae ) Kit Tan, Vural & Küçüködük Üzerinde Anatomik, Morfolojik ve Karyolojik Çalışmalar. Yüksek Lisans Tezi, Afyon Kocatepe Üniversitesi, Fen Bilimleri Enstitüsü. 2008.
  • Wıseman H, O’reılly JDO, Adlercreutz H, Mallet AI, Bowey EA, Rowland IR, Sanders TAB. Isoflavone phytoestrogens consumed in soy decrease F2-isoprostane concentrations and increase resistance of low-density lipoprotein to oxidation in humans. American Journal of Clinical Nutrition. 2000; 72: 395-400.
  • Yanez Rmb M, Mateos MRG, Hernández MRS, León TC, Kite G. Flavonoids and antioxidant activity of Calia secundilfora (Ort.) Yakovlev. Rev. fitotec. Mex. 2011; (34) 3.

Investigation of the Antioxidant Effects of Extract Obtained from Thermopsis turcica Plant in Rats

Year 2016, Volume: 9 Issue: 4, 259 - 265, 01.12.2016

Abstract

Thermopsis turcica, the endemic plant that belongs to fabaceae family, is only species that represents the type of Thermopsis in our country. Antioxidant effect of extracts obtained from plant Thermopsis Turcica is being aimed by this study. In the study, 3-4 months, weighing 250-350 g male Wistar albino rats were used in a total of 74. 64 of them were used to determine antioxidant activity, 10 of them were dose of ÖD50. Rats were divided into 8 groups and in each group there were 8 of them. The prepared extracts from the upper parts of the plant Thermopsis turcica that specified ÖD50 dose of about % 1, % 2 and % 4 in the rates , identified in the single daily doses which were based on groups, was performed simultaneously continued for 30 days by gastric gavage. At the end of the study , intracardiac blood, kidney and liver tissue samples were taken from the animals. From the samples, MDA, GSH, SOD, CAT and AOA levels were measured.The lethal dose 50 (ÖD50) ˃ 2000 mg/kg was found by ethanol and water extracts performance that were prepared from the upper parts of the plant Thermopsis turcica in the study. Lower values seen in whole blood MDA and were determined GSH low values in whole blood and tissue samples those were especially seen in aqueous extracts of the plant has positive effects on antioxidant effect. However, plasma AOA low values in both extracts caused an opinion about sufficient antioxidant effect didn’t occur.

References

  • Aebi H. Catalase in vitro assay methods. Methods Enzymol. 1984; 105. 121-126.
  • Aksoy L, Kolay E, Ağılönü Y, Aslan Z, Kargıoğlu M. Free Radical Scavenging Activity, Total Antioxidant Status and Total Oxidant Status of Endemic Thermopsis turcica, Saudi Journal of Biological Science. 2013; 10: 1016.
  • Bayır Y. Usnea Longıssıma Ach. Liken Türünden İzole Edilen Difraktaik Asit’ in İndometazin Ülseri Üzerine Koruyucu Etkisi ve İn-Vivo Antioksidan Özelliklerinin Araştırılması. Yüksek Lisans Tezi, Atatürk Üniv. Sağlık Bilimleri Enstitüsü. 2004.
  • Beutler E, Duron O, Kelly BM. Improved method for the determination of blood glutathione. Journal of Laboratory and Clinical Medicine. 1963; 61: 882-888.
  • Choudhary RK, Swarnkar PL. Antioxidant activity of phenolic and flavonoid compounds in some medicinal plants of india. Natural Product Research. 2011; 25 (11): 1101-1109.
  • Davis PH, Mill RR, Tan, Kıt. Flora of Turkey and the East Aegean Islands. University of Edinburg Press. Edinburg. 1988; 10: 112.
  • Dayan S. Endemik ve Tehlike Altındaki Thermopsis turcica (Fabaceae)’nın in vitro çimlenmesi ve mikroçogaltımı. Yüksek Lisans Tezi, Afyon Kocatepe Üniversitesi, Fen Bilimleri Enstitüsü. 2006.
  • Disilvestro RA, Goodman J, Dy E, Lavalle G. Soy isoflavone supplementation elevates erythrocyte superoxide dismutase, but not ceruloplasmin in postmenopausal breast cancer survivors. Breast Cancer Research and Treatment. 2005; 89: 251-255.
  • Draper HH, Hadley M. Malondialdehyde determination as index of lipid Peroxidation. Methods in Enzymology. 1990; 186: 421–431.
  • Dündar Y, Aslan R. Hekimlikte oksidatif stres ve antioksidanlar, Afyon Kocatepe Üniversitesi Yayınları. Afyon. 2000. Hu CC, Hsiao CH, Huang SY, Fu SH, Lai CC, Hong TM, Chen HH, Lu FJ. Antioxidant activity of fermented soybean extract. J Agric Food Chem. 2004; 52(18): 5735-9.
  • Ibrahim WH, Habib HM, Chow CK, Bruckner GG. Isoflavone-rich soy isolate reduces lipid peroxidation in mouse liver. Int J Vitam Nutr Res. 2008; 78(4-5): 217-22.
  • Khairunuur FA, Zulkhaırı A, Haıruszah I, Azrına A, Nursakınah I, Fazalı F, Kamal MNH, Zamree MS, Kamılah KAK. Hypolipemic and Weight Reducing Properties from Tamarindus indica L. Pulp Ekstract in Diet- İnduced Obese Rats. International Journal of Pharmacology. 2010; 6(3): 216-223. ISSN 1811-7775. Malaysia.
  • Koracevic D, Koracevic G, Djordjevic V, Andrejevic S, Cosic V. Method for the measurement of antioxidant activity in human fluids. J Clin Pathol. 2001; 54: 356-361.
  • Liu J, Chang SK, Wiesenborn D. Antioxidant properties of soybean isoflavone extract and tofu in vitro and in vivo. J Agric Food Chem. 2005; 53(6): 2333-40.
  • Luck H. Catalase. In: H.U. Bergmeyer (Eds.), Methods in Analysis, Academy Press, London. 1955.
  • Oecd. (2001). Guidelines for the Testing of Chemicals-425. [www.oecd.org/dataoecd, (02. 04. 2013)].
  • Okhawa Η, Onishi Ν, Yagi Κ. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal. Biochem., 1979; 95: 351.
  • Raja S, Ahamed HN, Kumar V, Mukherje K, Bandyopadhyay A, Mukherje PK. Exploring the Effect of Cytisus Scoparius on Markers of Oxidative Stress in Rats. Iranıan Journal of Pharmacology & Therapeutıcs. 2007; 6: 15-21.
  • Rajaram K, Moushmi M, Velayutham MDP, Kumpati P, Ganasaraswathi M, Sureshkumar P. Comparative Bioactive Studies Between Wild Plant and Callus Culture of Tephrosia tinctoria Pers. Appl Biochem Biotechnol. 2013; 0444-3: 12010-013. DOI 10.1007.
  • Rajeshwar Y, Gupta M, Mazumder UK. Antitumor Activity and in vivo Antioxidant Status of Mucuna pruriens (Fabaceae) Seeds against Ehrlich Ascites Carcinoma in Swiss Albino Mice. Iranıan Journal of Pharmacology & Therapeutıcs. 2005; 4: 46-53.
  • Sinan B. Thermopsis turcica Kit Tan, Vural & Küçüködük (Fabaceae)’nın Morfolojisi, Anatomisi ve Ekolojisi. Yüksek Lisans Tezi. Selçuk Üniversitesi, Fen Bilimleri Enstitüsü, Biyoloji Anabilim Dalı. 2002.
  • Sun Y, Oberley LW, Li Y. A simple method for clinical assay of superoxide dismutase. Clinical Chemistry. 1988; 34: 497–500.
  • Şerbetçi H. Meyan (Glycyrrhiza glabra L.) Bitkisinin Antioksidan Kapasitesinin Belirlenmesi. Yüksek Lisans Tezi. Atatürk Üniversitesi Fen Bilimleri Enstitüsü. 2007.
  • Tezcan S. Thermopsis turcica (Fabaceae ) Kit Tan, Vural & Küçüködük Üzerinde Anatomik, Morfolojik ve Karyolojik Çalışmalar. Yüksek Lisans Tezi, Afyon Kocatepe Üniversitesi, Fen Bilimleri Enstitüsü. 2008.
  • Wıseman H, O’reılly JDO, Adlercreutz H, Mallet AI, Bowey EA, Rowland IR, Sanders TAB. Isoflavone phytoestrogens consumed in soy decrease F2-isoprostane concentrations and increase resistance of low-density lipoprotein to oxidation in humans. American Journal of Clinical Nutrition. 2000; 72: 395-400.
  • Yanez Rmb M, Mateos MRG, Hernández MRS, León TC, Kite G. Flavonoids and antioxidant activity of Calia secundilfora (Ort.) Yakovlev. Rev. fitotec. Mex. 2011; (34) 3.
There are 26 citations in total.

Details

Journal Section RESEARCH ARTICLE
Authors

Yasemin Çelik

İsmail Küçükkurt This is me

Publication Date December 1, 2016
Acceptance Date October 10, 2016
Published in Issue Year 2016 Volume: 9 Issue: 4

Cite

APA Çelik, Y., & Küçükkurt, İ. (2016). Investigation of the Antioxidant Effects of Extract Obtained from Thermopsis turcica Plant in Rats. Kocatepe Veterinary Journal, 9(4), 259-265.
AMA Çelik Y, Küçükkurt İ. Investigation of the Antioxidant Effects of Extract Obtained from Thermopsis turcica Plant in Rats. kvj. December 2016;9(4):259-265.
Chicago Çelik, Yasemin, and İsmail Küçükkurt. “Investigation of the Antioxidant Effects of Extract Obtained from Thermopsis Turcica Plant in Rats”. Kocatepe Veterinary Journal 9, no. 4 (December 2016): 259-65.
EndNote Çelik Y, Küçükkurt İ (December 1, 2016) Investigation of the Antioxidant Effects of Extract Obtained from Thermopsis turcica Plant in Rats. Kocatepe Veterinary Journal 9 4 259–265.
IEEE Y. Çelik and İ. Küçükkurt, “Investigation of the Antioxidant Effects of Extract Obtained from Thermopsis turcica Plant in Rats”, kvj, vol. 9, no. 4, pp. 259–265, 2016.
ISNAD Çelik, Yasemin - Küçükkurt, İsmail. “Investigation of the Antioxidant Effects of Extract Obtained from Thermopsis Turcica Plant in Rats”. Kocatepe Veterinary Journal 9/4 (December 2016), 259-265.
JAMA Çelik Y, Küçükkurt İ. Investigation of the Antioxidant Effects of Extract Obtained from Thermopsis turcica Plant in Rats. kvj. 2016;9:259–265.
MLA Çelik, Yasemin and İsmail Küçükkurt. “Investigation of the Antioxidant Effects of Extract Obtained from Thermopsis Turcica Plant in Rats”. Kocatepe Veterinary Journal, vol. 9, no. 4, 2016, pp. 259-65.
Vancouver Çelik Y, Küçükkurt İ. Investigation of the Antioxidant Effects of Extract Obtained from Thermopsis turcica Plant in Rats. kvj. 2016;9(4):259-65.

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