Rekürren aftöz stomatitli hastalarda serum ve tükürükte dermcidin düzeylerinin araştırılması ve tükürük bezinde dermcidin analizi
Abstract
Amaç Rekürren aftöz stomatit (RAS) dudak mukozası, yanak ve dilde, tekrarlayıcı, küçük, ağrılı, eritemli
halesi bulunan, nekrotik ülserlerle karakterize, kendi kendini sınırlayabilen, sık görülen bir oral mukoza
hastalığıdır. Bu çalışmada RAS’lı hastaların serum ve tükürüğünde dermcidin düzeyinin, tükürük bezinde
dermcidin varlığının ve RAS patogenezindeki rolünün belirlenmesi amaçlandı.
Yöntem Bu çalışma 31 hasta ve 30 sağlıklı gönüllü ile yapıldı. Hasta ve kontrol grubunun serum ve tükürüğündeki dermcidin düzeyleri, insan dermcidin ELISA kitinin kataloglarında belirtilen çalışma prosedürlerine göre çalışıldı. Tükürük bezlerinde dermcidin varlığı immünohistokimyasal analiz ile
değerlendirildi.
Bulgular RAS’lı hasta grubunun ortalama tükürük dermcidin düzeyleri (105.80 ± 80.14), kontrol grubunun ortalama tükürük dermcidin düzeyleri (456.13 ± 354.59) ile kıyaslandığında istatistiksel anlamlı farklılık tespit edildi (P=0.000). RAS’lı hasta grubunda ortalama serum dermcidin düzeyleri (316.41 ± 784.55), kontrol grubunun ortalama serum dermcidin düzeyleri (130.65 ± 179.75) ile kıyaslandığında istatistiksel anlamlı farklılık tespit edilemedi. İmmünohistokimyasal boyamada parotis ve submandibular bezlerde, interlobular kanallarda dermcidin immünreaktivitesi gözlendi.
Sonuç Sonuç olarak bu çalışmada tükürük bezinin striated hücrelerinin dermcidin sentezlediği ortaya
kondu. Tükürükte antimikrobial özellikli dermcidin azlığının RAS için predispozan bir faktör olduğu düşünüldü.
Keywords
References
- Belenguer-Guallar I, Jimenez-Soriano Y, Claramunt-Lozano A. Treatment of recurrent aphthous stomatitis. A literature review. J Clin Exp Dent 2014;6:168-74.
- Slebioda Z, Szponar E, Kowalska A. Etiopathogenesis of recurrent aphthous stomatitis and the role of immunologic aspects: literature review. Arch Immunol Ther Exp (Warsz) 2014;62:205-15.
- Chavan M, Jain H, Diwan N, Khedkar S, Shete A, Durkar S. Recurrent aphthous stomatitis: a review. J Oral Pathol Med 2012;41:577-83.
- Schittek B, Hipfel R, Sauer B, et al. Dermcidin: a novel human antibiotic peptide secreted by sweat glands. Nat Immunol 2001;2:1133-7.
- Mehra T, Koberle M, Braunsdorf C, Mailander-Sanchez D, Borelli C, Schaller M. Alternative approaches to antifungal therapies. Exp Dermatol 2012;21:778-82.
- Vuong C, Voyich JM, Fischer ER, et al. Polysaccharide intercellular adhesin (PIA) protects Staphylococcus epidermidis against major components of the human innate immune system. Cell Microbiol 2004;6:269-75.
- Lai YP, Peng YF, Zuo Y, et al. Functional and structural characterization of recombinant dermcidin-1L, a human antimicrobial peptide. Biochem Biophys Res Commun 2005;328:243-50.
- Nakano T, Yoshino T, Fujimura T, et al. Reduced expression of dermcidin, a peptide active against propionibacterium acnes, in sweat of patients with acne vulgaris. Acta Derm Venereol 2015;95:783-6.
- Arai S, Yoshino T, Fujimura T, et al. Mycostatic effect of recombinant dermcidin against Trichophyton rubrum and reduced dermcidin expression in the sweat of tinea pedis patients. J Dermatol 2015;42:70-6.
- Hsu SM, Raine L, Fanger H. Use of avidin-biotinperoxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 1981;29:577-80. Aydin S. A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides 2015;72:4-15.
- Holmberg KV, Hoffman MP. Anatomy, biogenesis and regeneration of salivary glands. Monogr Oral Sci 2014;24:1-13.
- Dawes C, Pedersen AM, Villa A, et al. The functions of human saliva: a review sponsored by the World Workshop on Oral Medicine VI. Arch Oral Biol 2015;60:863-74.
- van’t Hof W, Veerman EC, Nieuw Amerongen AV, Ligtenberg AJ. Antimicrobial defense systems in saliva. Monogr Oral Sci 2014;24:40-51.
- Dale BA, Tao R, Kimball JR, Jurevic RJ. Oral antimicrobial peptides and biological control of caries. BMC Oral Health 2006;6:13.
- Denny P, Hagen FK, Hardt M, et al. The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions. J Proteome Res 2008;7:1994-2006.
- Preza D, Thiede B, Olsen I, Grinde B. The proteome of the human parotid gland secretion in elderly with and without root caries. Acta Odontol Scand 2009;67:161-9.
- Rieg S, Seeber S, Steffen H, et al. Generation of multiple stable dermcidin-derived antimicrobial peptides in sweat of different body sites. J Invest Dermatol 2006;126:354-65.
- Shaw JL, Smith CR, Diamandis EP. Proteomic analysis of human cervico-vaginal fluid. J Proteome Res 2007;6:2859-65.
- You J, Fitzgerald A, Cozzi PJ, et al. Post-translation modification of proteins in tears. Electrophoresis 2010;31:1853-61.
- Schittek B. The multiple facets of dermcidin in cell survival and host defense. J Innate Immun 2012;4:349-60.
- Wiesner J, Vilcinskas A. Antimicrobial peptides: the ancient arm of the human immune system. Virulence 2010;1:440-64.
Investigation of serum and saliva dermcidin levels in patients with recurrent aphthous stomatitis and dermcidin analysis in salivary gland
Abstract
Objective Recurrent aphthous stomatitis (RAS) is a common self-limiting oral mucosa disease. In this study, it was aimed to determine the dermcidin level in the serum and saliva of patients with RAS, the presence of dermcidin in the salivary gland and its role in the pathogenesis of RAS.
Methods Thirty-one patients presenting with RAS and 30 control subjects participated in this study. Dermcidin levels in serum and saliva of patients and control group were studied in accordance with the working procedures specified in the catalogs of the human dermcidin ELISA kit. The presence of dermcidin in salivary glands was assessed by immunohistochemical analysis.
Results A statistically significant difference was found when the mean salivary dermcidin levels (105.80 ± 80.14 ng/mL) of the RAS patients were compared with the mean salivary dermcidin levels (456.13 ± 354.59 ng/mL) of the control group (P=0.000). There was no statistically significant difference between the mean serum dermcidin levels (316.41 ± 784.55 ng/mL) of the RAS patient and those of (130.65 ± 179.75 ng/mL) the control group. Dermcidin immunoreactivity was observed in the parotid gland, submandibular gland and interlobular striated ducts.
Conclusion The findings in this study showed that striated cells in salivary gland synthesized dermcidin. Low
levels of dermcidin with antimicrobial properties in saliva were considered as a predisposing factor for RAS.
References
- Belenguer-Guallar I, Jimenez-Soriano Y, Claramunt-Lozano A. Treatment of recurrent aphthous stomatitis. A literature review. J Clin Exp Dent 2014;6:168-74.
- Slebioda Z, Szponar E, Kowalska A. Etiopathogenesis of recurrent aphthous stomatitis and the role of immunologic aspects: literature review. Arch Immunol Ther Exp (Warsz) 2014;62:205-15.
- Chavan M, Jain H, Diwan N, Khedkar S, Shete A, Durkar S. Recurrent aphthous stomatitis: a review. J Oral Pathol Med 2012;41:577-83.
- Schittek B, Hipfel R, Sauer B, et al. Dermcidin: a novel human antibiotic peptide secreted by sweat glands. Nat Immunol 2001;2:1133-7.
- Mehra T, Koberle M, Braunsdorf C, Mailander-Sanchez D, Borelli C, Schaller M. Alternative approaches to antifungal therapies. Exp Dermatol 2012;21:778-82.
- Vuong C, Voyich JM, Fischer ER, et al. Polysaccharide intercellular adhesin (PIA) protects Staphylococcus epidermidis against major components of the human innate immune system. Cell Microbiol 2004;6:269-75.
- Lai YP, Peng YF, Zuo Y, et al. Functional and structural characterization of recombinant dermcidin-1L, a human antimicrobial peptide. Biochem Biophys Res Commun 2005;328:243-50.
- Nakano T, Yoshino T, Fujimura T, et al. Reduced expression of dermcidin, a peptide active against propionibacterium acnes, in sweat of patients with acne vulgaris. Acta Derm Venereol 2015;95:783-6.
- Arai S, Yoshino T, Fujimura T, et al. Mycostatic effect of recombinant dermcidin against Trichophyton rubrum and reduced dermcidin expression in the sweat of tinea pedis patients. J Dermatol 2015;42:70-6.
- Hsu SM, Raine L, Fanger H. Use of avidin-biotinperoxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 1981;29:577-80. Aydin S. A short history, principles, and types of ELISA, and our laboratory experience with peptide/protein analyses using ELISA. Peptides 2015;72:4-15.
- Holmberg KV, Hoffman MP. Anatomy, biogenesis and regeneration of salivary glands. Monogr Oral Sci 2014;24:1-13.
- Dawes C, Pedersen AM, Villa A, et al. The functions of human saliva: a review sponsored by the World Workshop on Oral Medicine VI. Arch Oral Biol 2015;60:863-74.
- van’t Hof W, Veerman EC, Nieuw Amerongen AV, Ligtenberg AJ. Antimicrobial defense systems in saliva. Monogr Oral Sci 2014;24:40-51.
- Dale BA, Tao R, Kimball JR, Jurevic RJ. Oral antimicrobial peptides and biological control of caries. BMC Oral Health 2006;6:13.
- Denny P, Hagen FK, Hardt M, et al. The proteomes of human parotid and submandibular/sublingual gland salivas collected as the ductal secretions. J Proteome Res 2008;7:1994-2006.
- Preza D, Thiede B, Olsen I, Grinde B. The proteome of the human parotid gland secretion in elderly with and without root caries. Acta Odontol Scand 2009;67:161-9.
- Rieg S, Seeber S, Steffen H, et al. Generation of multiple stable dermcidin-derived antimicrobial peptides in sweat of different body sites. J Invest Dermatol 2006;126:354-65.
- Shaw JL, Smith CR, Diamandis EP. Proteomic analysis of human cervico-vaginal fluid. J Proteome Res 2007;6:2859-65.
- You J, Fitzgerald A, Cozzi PJ, et al. Post-translation modification of proteins in tears. Electrophoresis 2010;31:1853-61.
- Schittek B. The multiple facets of dermcidin in cell survival and host defense. J Innate Immun 2012;4:349-60.
- Wiesner J, Vilcinskas A. Antimicrobial peptides: the ancient arm of the human immune system. Virulence 2010;1:440-64.
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Original Articles |
| Authors | |
| Publication Date | March 31, 2021 |
| Published in Issue | Year 2021 Volume: 4 Issue: 1 |
