Klinik Araştırma
Yıl 2023,
Cilt: 6 Sayı: 3, 390 - 394, 21.10.2023
Öz
Kaynakça
- Ombelet W. Global access to infertility care in developing countries: a case of human rights, equity and social justice. Facts Views Vis Obgyn. 2011;3(4):257-266.
- Kumar N, Singh AK. Trends of male factor infertility, an important cause of infertility: A review of literature. J Hum Reprod Sci. 2015;8(4):191-196. doi:10.4103/0974-1208.170370
- Cooper TG, Noonan E, von Eckardstein S, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update. 2010;16(3):231‐245. doi:10.1093/humupd/dmp048
- Wang C, Swerdloff RS. Limitations of semen analysis as a test of male fertility and anticipated needs from newer tests. Fertil Steril. 2014;102(6):1502-1507. doi:10.1016/j.fertnstert.2014.10.021
- Oehninger S, Ombelet W. Limits of current male fertility testing. Fertil Steril. 2019;111(5):835-841. doi:10.1016/j.fertnstert.2019.03.005
- Hammadeh ME, Zeginiadov T, Rosenbaum P, Georg T, Schmidt W, Strehler E. Predictive value of sperm chromatin condensation (aniline blue staining) in the assessment of male fertility. Arch Androl. 2001;46(2):99-104.
- Khalili MA, Nazari S, Dehghani-Firouzabadi R, et al. Comparing the roles of sperm chromatin integrity and apoptosis in intrauterine insemination outcomes of couples with mild male and female factor infertility. J Reprod Infertil. 2014;15(1):35-40.
- Saleh RA, Agarwal A, Nelson DR, et al. Increased sperm nuclear DNA damage in normozoospermic infertile men: a prospective study. Fertil Steril. 2002;78(2):313-318. doi:10.1016/s0015-0282(02)03219-3
- Agarwal A, Allamaneni SS. Sperm DNA damage assessment: a test whose time has come. Fertil Steril. 2005;84(4):850-853. doi:10.1016/j.fertnstert.2005.03.080
- Softness KA, Trussler JT, Carrasquillo RJ. Advanced sperm testing. Curr Opin Urol. 2020;30(3):290-295. doi:10.1097/MOU.0000000000000761
- Aitken RJ, De Iuliis GN. On the possible origins of DNA damage in human spermatozoa. Mol Hum Reprod. 2010;16(1):3-13. doi:10.1093/molehr/gap059
- Franken DR, Franken CJ, de la Guerre H, de Villiers A. Normal sperm morphology and chromatin packaging: comparison between aniline blue and chromomycin A3 staining. Andrologia. 1999;31(6):361-366. doi:10.1046/j.1439-0272.1999.00290.x
- Irez T, Sahmay S, Ocal P, et al. Investigation of the association between the outcomes of sperm chromatin condensation and decondensation tests, and assisted reproduction techniques. Andrologia. 2015;47(4):438-447. doi:10.1111/and.12286
- Ribas-Maynou J, García-Peiró A, Fernández-Encinas A, et al. Comprehensive analysis of sperm DNA fragmentation by five different assays: TUNEL assay, SCSA, SCD test and alkaline and neutral Comet assay. Andrology. 2013;1(5):715-722. doi:10.1111/j.2047-2927.2013.00111.x
- Atshan M, Kakavand K, Hosseini SH, Sadighi Gilani MA, Mohseni Meybodi A, Sabbaghian M. Evaluation of sperm DNA fragmentation and chromatin structure in infertile men with immotile short-tail sperm defect. Andrologia. 2020;52(1). doi:10.1111/and.13445
- Hologlu D, Gunes S, Asci R, Henkel R, Guvenc T. Association among sperm chromatin condensation, sperm DNA fragmentation and 8-OHdG in seminal plasma and semen parameters in infertile men with oligoasthenoteratozoospermia. Andrologia. 2022;54(1): e14268. doi:10.1111/and.14268
- Salsabili N, Mehrsai A, Jalalizadeh B, Pourmand G, Jalaie S. Correlation of sperm nuclear chromatin condensation staining method with semen parameters and sperm functional tests in patients with spinal cord injury, varicocele, and idiopathic infertility. Urol J. 2006;3(1):32-37.
- Sánchez-Álvarez J, Cano-Corres R, Fuentes-Arderiu X. A Complement for the WHO Laboratory Manual for the Examination and Processing of Human Semen (First Edition, 2010). EJIFCC. 2012;23(3):103-106. Published 2012 Oct 12.
- Menkveld R, Stander FS, Kotze TJ, Kruger TF, van Zyl JA. The evaluation of morphological characteristics of human spermatozoa according to stricter criteria. Hum Reprod. 1990;5(5):586-592. doi:10.1093/oxfordjournals.humrep.a137150
- Hammadeh ME, al-Hasani S, Stieber M, et al. The effect of chromatin condensation (aniline blue staining) and morphology (strict criteria) of human spermatozoa on fertilization, cleavage and pregnancy rates in an intracytoplasmic sperm injection programme. Hum Reprod. 1996;11(11):2468-2471. doi:10.1093/oxfordjournals.humrep.a019139
- Aitken RJ, Baker MA, Sawyer D. Oxidative stress in the male germ line and its role in the aetiology of male infertility and genetic disease. Reprod Biomed Online. 2003;7(1):65-70. doi:10.1016/s1472-6483(10)61730-0
- Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update. 2003;9(4):331-345. doi:10.1093/humupd/dmg027
- Muriel L, Garrido N, Fernández JL, et al. Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization and intracytoplasmic sperm injection. Fertil Steril. 2006;85(2):371-383. doi:10.1016/j.fertnstert.2005.07.1327
- Sadek A, Almohamdy AS, Zaki A, Aref M, Ibrahim SM, Mostafa T. Sperm chromatin condensation in infertile men with varicocele before and after surgical repair. Fertil Steril. 2011;95(5):1705-1708. doi:10.1016/j.fertnstert.2011.01.008
SPERM DNA BÜTÜNLÜĞÜNÜN SİTOKİMYASAL DEĞERLENDİRİLMESİ VE İDİOPATİK İNFERTİL ERKEKLERDE KONVANSİYONEL SEMEN PARAMETRELERİ İLE İLİŞKİSİ
Öz
ÖZ
Amaç: Bu çalışmanın amacı idiyopatik infertil erkeklerde sperm DNA bütünlüğü ile konvansiyonel semen parametreleri arasındaki ilişkinin sitokimyasal yöntemlerle araştırılmasıdır.
Yöntem: 40 idiopatik infertilitesi olan bireyde, nükleer kromatin yoğunlaşmasını belirten asidik anilin mavisi boyama yöntemi ve sperm DNA kırıklarını belirten TUNEL yöntemi kullanılarak sperm DNA bütünlüğü değerlendirildi. Yayma preperatlarda, sperm DNA hasarı gösteren TUNEL+ ve anilin blue+ boyalı sperm başları sayıldı. Bulgular, rutin semen parametreleri ile sperm DNA bütünlük testleri sonuçları arasındaki ilişkiyi belirlemek için kullanıldı.
Bulgular: Anilin mavisi+ boyanma oranı ile sperm morfolojisi ve progresif hareketliliği arasında anlamlı bir negatif korelasyon izlendi. TUNEL+ boyanma oranı ile normal morfoloji ve ilerleyici hareketlilik arasında negatif bir korelasyon gözlendi (P<0.01), ancak sperm konsantrasyonu ve sperm toplam hareketliliği ile ilgili herhangi bir korelasyon bulunamadı.
Sonuç: Asidik anilin mavisi ve TUNEL gibi yöntemler, geleneksel semen parametrelerinden bağımsız olarak spermin yapısal kusurlarını gösterebilmektedir. Bu yöntemler bazı semen parametreleri ile ilişkili olsa da özellikle idiyopatik infertil hasta grubu için kullanılması yardımcı üreme tekniklerinde başarıya olumlu katkı sağlayabilir.
Anahtar Kelimeler
Sperm Kromatin yoğunlaşması DNA fragmantasyonu TUNEL Anilin mavisi
Kaynakça
- Ombelet W. Global access to infertility care in developing countries: a case of human rights, equity and social justice. Facts Views Vis Obgyn. 2011;3(4):257-266.
- Kumar N, Singh AK. Trends of male factor infertility, an important cause of infertility: A review of literature. J Hum Reprod Sci. 2015;8(4):191-196. doi:10.4103/0974-1208.170370
- Cooper TG, Noonan E, von Eckardstein S, et al. World Health Organization reference values for human semen characteristics. Hum Reprod Update. 2010;16(3):231‐245. doi:10.1093/humupd/dmp048
- Wang C, Swerdloff RS. Limitations of semen analysis as a test of male fertility and anticipated needs from newer tests. Fertil Steril. 2014;102(6):1502-1507. doi:10.1016/j.fertnstert.2014.10.021
- Oehninger S, Ombelet W. Limits of current male fertility testing. Fertil Steril. 2019;111(5):835-841. doi:10.1016/j.fertnstert.2019.03.005
- Hammadeh ME, Zeginiadov T, Rosenbaum P, Georg T, Schmidt W, Strehler E. Predictive value of sperm chromatin condensation (aniline blue staining) in the assessment of male fertility. Arch Androl. 2001;46(2):99-104.
- Khalili MA, Nazari S, Dehghani-Firouzabadi R, et al. Comparing the roles of sperm chromatin integrity and apoptosis in intrauterine insemination outcomes of couples with mild male and female factor infertility. J Reprod Infertil. 2014;15(1):35-40.
- Saleh RA, Agarwal A, Nelson DR, et al. Increased sperm nuclear DNA damage in normozoospermic infertile men: a prospective study. Fertil Steril. 2002;78(2):313-318. doi:10.1016/s0015-0282(02)03219-3
- Agarwal A, Allamaneni SS. Sperm DNA damage assessment: a test whose time has come. Fertil Steril. 2005;84(4):850-853. doi:10.1016/j.fertnstert.2005.03.080
- Softness KA, Trussler JT, Carrasquillo RJ. Advanced sperm testing. Curr Opin Urol. 2020;30(3):290-295. doi:10.1097/MOU.0000000000000761
- Aitken RJ, De Iuliis GN. On the possible origins of DNA damage in human spermatozoa. Mol Hum Reprod. 2010;16(1):3-13. doi:10.1093/molehr/gap059
- Franken DR, Franken CJ, de la Guerre H, de Villiers A. Normal sperm morphology and chromatin packaging: comparison between aniline blue and chromomycin A3 staining. Andrologia. 1999;31(6):361-366. doi:10.1046/j.1439-0272.1999.00290.x
- Irez T, Sahmay S, Ocal P, et al. Investigation of the association between the outcomes of sperm chromatin condensation and decondensation tests, and assisted reproduction techniques. Andrologia. 2015;47(4):438-447. doi:10.1111/and.12286
- Ribas-Maynou J, García-Peiró A, Fernández-Encinas A, et al. Comprehensive analysis of sperm DNA fragmentation by five different assays: TUNEL assay, SCSA, SCD test and alkaline and neutral Comet assay. Andrology. 2013;1(5):715-722. doi:10.1111/j.2047-2927.2013.00111.x
- Atshan M, Kakavand K, Hosseini SH, Sadighi Gilani MA, Mohseni Meybodi A, Sabbaghian M. Evaluation of sperm DNA fragmentation and chromatin structure in infertile men with immotile short-tail sperm defect. Andrologia. 2020;52(1). doi:10.1111/and.13445
- Hologlu D, Gunes S, Asci R, Henkel R, Guvenc T. Association among sperm chromatin condensation, sperm DNA fragmentation and 8-OHdG in seminal plasma and semen parameters in infertile men with oligoasthenoteratozoospermia. Andrologia. 2022;54(1): e14268. doi:10.1111/and.14268
- Salsabili N, Mehrsai A, Jalalizadeh B, Pourmand G, Jalaie S. Correlation of sperm nuclear chromatin condensation staining method with semen parameters and sperm functional tests in patients with spinal cord injury, varicocele, and idiopathic infertility. Urol J. 2006;3(1):32-37.
- Sánchez-Álvarez J, Cano-Corres R, Fuentes-Arderiu X. A Complement for the WHO Laboratory Manual for the Examination and Processing of Human Semen (First Edition, 2010). EJIFCC. 2012;23(3):103-106. Published 2012 Oct 12.
- Menkveld R, Stander FS, Kotze TJ, Kruger TF, van Zyl JA. The evaluation of morphological characteristics of human spermatozoa according to stricter criteria. Hum Reprod. 1990;5(5):586-592. doi:10.1093/oxfordjournals.humrep.a137150
- Hammadeh ME, al-Hasani S, Stieber M, et al. The effect of chromatin condensation (aniline blue staining) and morphology (strict criteria) of human spermatozoa on fertilization, cleavage and pregnancy rates in an intracytoplasmic sperm injection programme. Hum Reprod. 1996;11(11):2468-2471. doi:10.1093/oxfordjournals.humrep.a019139
- Aitken RJ, Baker MA, Sawyer D. Oxidative stress in the male germ line and its role in the aetiology of male infertility and genetic disease. Reprod Biomed Online. 2003;7(1):65-70. doi:10.1016/s1472-6483(10)61730-0
- Agarwal A, Said TM. Role of sperm chromatin abnormalities and DNA damage in male infertility. Hum Reprod Update. 2003;9(4):331-345. doi:10.1093/humupd/dmg027
- Muriel L, Garrido N, Fernández JL, et al. Value of the sperm deoxyribonucleic acid fragmentation level, as measured by the sperm chromatin dispersion test, in the outcome of in vitro fertilization and intracytoplasmic sperm injection. Fertil Steril. 2006;85(2):371-383. doi:10.1016/j.fertnstert.2005.07.1327
- Sadek A, Almohamdy AS, Zaki A, Aref M, Ibrahim SM, Mostafa T. Sperm chromatin condensation in infertile men with varicocele before and after surgical repair. Fertil Steril. 2011;95(5):1705-1708. doi:10.1016/j.fertnstert.2011.01.008
Toplam 24 adet kaynakça vardır.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Üreme Tıbbı (Diğer) |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 21 Ekim 2023 |
| Gönderilme Tarihi | 14 Ağustos 2023 |
| Kabul Tarihi | 18 Eylül 2023 |
| Yayımlandığı Sayı | Yıl 2023 Cilt: 6 Sayı: 3 |
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