Abstract
Purpose:Using preservative urine tubes for urine analysis has been made a new era. A proportion of compounds in a preservative tube can be listed as follows: Sodium Propionate (94%), ethylparaben (5.6%) and chlorhexidine (0.4%).
Patients and Methods:We study the urinary tube containing preservative (Becton-Dickinson, Franklin Lakes, USA) additives aimed to study whether the effect on some biochemical tests. 24-h urine samples (n=118) were obtained from outpatients for routine urine analysis. They were divided in to the two tubes: containing preservatives tubes and additive free tubes. Six biochemistry parameters (protein, calcium, urea, phosphorus, creatinine, microalbumin) were tested on Beckman Coulter AU680 analyzer.
Results:Results: The results of phosphor in two urine samples were decreased significantly in all of the time points in stability studies(p= 0.02, p < 0.01).
Conclusion:Conclusion: We can conclude that tubes containing preservatives could be used to analyze biochemical parameters which were analyzed. But urine phosphor measurement with the additive tubes should be done promptly after delivery to the laboratory.
Keywords
References
- 1. Coppens A, Speeckaert M, Delanghe J. The pre-analytical challenges of routine urinalysis. Acta Clinica Belgica 2010; 65:182-189.
- 2. Fogazzi GB, Cameron JS, Ritz E, Ponticelli C. The history of urinary microscopy to the end of the 19th century. Am J Nephrol 1994; 14:452-7.
- 3. Delanghe J, Speeckaert M. Preanalytical requirements of urinalysis. Biochem Medica 2014; 24:89-104.
- 4.Yilmaz G, Yilmaz FM, Yucel D. A preservatives necessary in 24-hour urine measurements? Clinical Biochemistry 2008:41;899-901.
- 5. Lojo SA, Soto S, Lojo-Luaces N. Validation of the Vacutainer® urinalysis preservative plus urine tube for the determination of albumin and protein.[Epub ahead of print] Clin Chem Lab Med 2016 as doi: 10.1515/cclm-2015-1033.
- 6. Eisinger SW, Schwartz M, Dam L, Riedel S. Evaluation of the BD vacutainer plus urine C&S preservative tubes compared with non-preservative urine samples stored at 4°C and room temperature. Am J Clin Pathol 2013; 140:306-13.
- 7. Fogazzi GB, Verdesca S, Garigali G. Urinalysis: Core Curriculum 2008. Core Curriculum in Nephrology. Am J KidneyDis 2008; 51:1052- 67.
Abstract
Amaç:İdrar analizi için koruyucu içeren idrar tüplerinin kullanılması yeni bir dönem olmuştur. Koruyucu içeren tüplerdeki bileşikler şu şekilde sıralanabilir: Sodyum Propiyonat (% 94), etilparaben (% 5.6) ve klorheksidin (% 0.4).
Hastalar ve Yöntem:Biz çalışmamızda koruyucu içeren idrar tüplerinin (Becton-Dickinson, Franklin Lakes, USA) bazı biyokimyasal parametreler üzerine etkisini incelemeyi amaçladık. Rutin idrar analizi için gelen 24 saatlik idrar örneklerinden 118 tanesi çalışmaya alındı. İdrar örnekleri 2’ye porsiyonlandı: Koruyucu içeren tüpler ve katkı maddesi içermeyen tüpler. Beckman Coulter AU680 analizöründe altı biyokimya parametresi (protein, kalsiyum, üre, fosfor, kreatinin, mikroalbümin) test edildi.
Bulgular:İki idrar örneğindeki fosforun sonuçları stabilite çalışmalarında tüm zaman noktalarında anlamlı olarak azaldı (p = 0.02, p <0.01).
Sonuç:Biyokimyasal parametreleri analiz etmek için koruyucu içeren tüplerin kullanılabileceği sonucuna varabiliriz. Ancak, koruyucu içeren tüplerle idrar fosfor ölçümü laboratuvara teslim edildikten hemen sonra yapılmalıdır.
References
- 1. Coppens A, Speeckaert M, Delanghe J. The pre-analytical challenges of routine urinalysis. Acta Clinica Belgica 2010; 65:182-189.
- 2. Fogazzi GB, Cameron JS, Ritz E, Ponticelli C. The history of urinary microscopy to the end of the 19th century. Am J Nephrol 1994; 14:452-7.
- 3. Delanghe J, Speeckaert M. Preanalytical requirements of urinalysis. Biochem Medica 2014; 24:89-104.
- 4.Yilmaz G, Yilmaz FM, Yucel D. A preservatives necessary in 24-hour urine measurements? Clinical Biochemistry 2008:41;899-901.
- 5. Lojo SA, Soto S, Lojo-Luaces N. Validation of the Vacutainer® urinalysis preservative plus urine tube for the determination of albumin and protein.[Epub ahead of print] Clin Chem Lab Med 2016 as doi: 10.1515/cclm-2015-1033.
- 6. Eisinger SW, Schwartz M, Dam L, Riedel S. Evaluation of the BD vacutainer plus urine C&S preservative tubes compared with non-preservative urine samples stored at 4°C and room temperature. Am J Clin Pathol 2013; 140:306-13.
- 7. Fogazzi GB, Verdesca S, Garigali G. Urinalysis: Core Curriculum 2008. Core Curriculum in Nephrology. Am J KidneyDis 2008; 51:1052- 67.
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | December 16, 2020 |
| Submission Date | May 10, 2019 |
| Published in Issue | Year 2021 |