Öz
Kaynakça
- van de Merwe JP, Nordling J, Bouchelouche P, Bouchelouche K, Cervigni M, Daha LK, et al. Diagnostic criteria, classification, and nomenclature for painful bladder syndrome/interstitial cystitis: an ESSIC proposal. Eur Urol. 2008; 53(1):60-7.
Öz
Objective: The aim of the study was to investigate the role of montelukast (ML), a
cysteinyl leukotriene-1 receptor antagonist, on the water avoidance stress (WAS) –
induced degeneration of the rat urinary bladder mucosa.
Methods: In WAS group, rats were exposed to WAS two hours daily for five days.
In WAS+ML group, rats were administered ML (10 mg/kg; i.p.;) following every WAS
exposure for 5 days. In control group, rats were injected vehicle solution only. The
urinary bladder was evaluated for general morphology at light microscope. Mast
cell activation and uroplakin distribution were assassed with immunohistochemistry.
Glycosaminoglycan (GAG) distribution and urothelial permeability were observed
using ruthenium red (RR) staining techniques in transmission electron microscope
and luminal urothelial cells were observed with scanning electron microscope. Tissue
malondialdehyde (MDA) and gluthatione (GSH) levels were also analysed.
Results: Irregular GAG layer and uroplakin distribution, penetration of RR in
the intercellular spaces and dilated tight junctions in urothelial layer, increase in
inflammatory cell infiltration, in number of both granulated and activated mast cells,
and were observed in the WAS group. The MDA level was increased, and GSH level
was decreased significantly in urinary bladder in the WAS group in comparison with
the control group. Quite regular GAG layer, uroplakin distribution and tight junctions
in most regions, decrease in inflammatory cell infiltration and both of activated and
granulated mast cells in the mucosa, were observed in WAS+ML group. Moreover,
significant decrease in MDA and increase in GSH levels were observed in this group.
Conclusion: Montelukast appears to exert a protective activity in WAS induced
urinary bladder injury by inhibiting inflammatory and oxidative activity.
Anahtar Kelimeler
Water avoidance stress montelukast urinary bladder uroplakin mast cell
Kaynakça
- van de Merwe JP, Nordling J, Bouchelouche P, Bouchelouche K, Cervigni M, Daha LK, et al. Diagnostic criteria, classification, and nomenclature for painful bladder syndrome/interstitial cystitis: an ESSIC proposal. Eur Urol. 2008; 53(1):60-7.
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Sağlık Kurumları Yönetimi |
| Bölüm | Articles |
| Yazarlar | |
| Yayımlanma Tarihi | 28 Eylül 2018 |
| Gönderilme Tarihi | 19 Temmuz 2017 |
| Yayımlandığı Sayı | Yıl 2018 Cilt: 8 Sayı: 3 |
Kaynak Göster
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