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The Antioxidant Effects of Sesamol on Bleomycin-Induced Oral Submucous Fibrosis

Yıl 2022, Cilt: 12 Sayı: 3, 238 - 242, 31.12.2022
https://doi.org/10.26650/experimed.1195246

Öz

Objective: Oral submucous fibrosis (OSF) is a disease characterized by abnormal collagen deposition that causes inflammation and malignancy in oral mucosal tissue. Fibroblasts and myofibroblasts, are the cells that show significant activation in the development of OSF. Bleomycin (BL) is a chemotherapeutic agent commonly used in cancer treatment that also causes inflammatory changes in the oral mucosa, initiating fibrosis in the oral submucosal tissue. Sesamol (SE) has antioxidative and anti-inflammatory properties which are abundant in sesame seeds and sesame oil, and SE has a beneficial effect on the mucosal layer. This experimental study aimed to investigate SE’s effects on BL-induced OSF.

Materials and Methods: The study obtained 18 healthy adult male albino rats aged 3-4 months and weighing 200-250g from Bolu Abant Izzet Baysal University’s Experimental Animal Application and Research Center. The rats were divided randomly into control, BL, and BL + SE groups (n = 6 in each group). A model of OSF was established in the rats by administering 0.5 mg/mL of BL and 50 mg\kg of SE to the BL+SE group each day. The hematoxylin and eosin stain and Masson’s trichrome stain were used to assess histopathological changes in the oral mucosa.

Results: The results revealed SE to have beneficial effects on BL-induced OSF through its antioxidant and anti-inflammatory properties. Histopathological evaluations and biochemical analysis of oral submucosal tissue samples also revealed SE to provide statistically significant protection against fibrosis in the oral mucosa (p < 0.01).

Conclusion: This study has demonstrated oral submucous fibrosis that develops due to BL as well as SE to have an antioxidant effect on OSF regarding BL-induced reactive oxygen species (ROS) activation and collagen synthesis

Teşekkür

We thanks to Prof.Dr. Mehmet Aydın for his contribution to the statistical analysis of this study.

Kaynakça

  • 1. World Health Organization; International Agency for Research on Cancer. IARC monograph on the evaluation of carcinogenic risks to humans, betel-quid and areca-nut chewing and some arecanut-derived nitrosamines. Vol. 85. Lyon, France: IARC; 2004. google scholar
  • 2. Arakeri G, Brennan PA. Oral submucous fibrosis: An overview of the aetiology, pathogenesis, classification, and principles of management. Br J Oral Maxillofac Surg 2013; 51: 587-93. [CrossRef] google scholar
  • 3. Angadi PV, Kale AD, Hallikerimath S. Evaluation of myofibroblasts in oral submucous fibrosis: correlation with disease severity. J Oral Pathol Med 2011; 40: 208-13. [CrossRef] google scholar
  • 4. Kerr AR, Warnakulasuriya S, Mighell AJ, T Dietrich, M Nasser, J Rimal, et al. A systematic review of medical interventions for oral submucous fibrosis and future research opportunities. Oral Dis 2011; 17: 42-57. [CrossRef] google scholar
  • 5. Jiang XW, Zhang Y, Yang SK, Zhang H, Lu K, Sun GL. Efficacy of salvianolic acid B combined with triamcinolone acetonide in the treatment of oral submucous fibrosis. Oral Surg Oral Med Oral Pathol Oral Radiol 2013; 115: 339-44. [CrossRef] google scholar
  • 6. Chua F, Gauldie J, Laurent GJ. Pulmonary fibrosis: Searching for model answers. Am J Respir Cell Mol Biol 2005; 33: 9-13. [CrossRef] google scholar
  • 7. Yamamoto T, Takagawa S, Katayama I, Yamazaki K, Hamazaki Y, Shinkai H, et al. Animal model of sclerotic skin. I: Local injections of bleomycin induce sclerotic skin mimicking scleroderma. J Invest Dermatol 1999; 112: 456-62. [CrossRef] google scholar
  • 8- Zhang SS, Gong ZJ, Xiong W, Wang X, Min Q, Luo CD, et al. A rat model of oral submucous fibrosis induced by bleomycin. Oral Surg Oral Med Oral Pathol Oral Radiol 2016; 122(2): 216-23. [CrossRef] google scholar
  • 9- Kabel AM, Omar MS, Elmaaboud MAA. Amelioration of bleomycin-induced lung fibrosis in rats by valproic acid and butyrate: Role of nuclear factor kappa-B, proinflammatory cytokines and oxidative stress. Int Immunopharmacol 2016; 39: 335-42. [CrossRef] google scholar
  • 10. Jan KC, Ho CT, Hwang LS. Bioavailability and tissue distribution of sesamol in rat. J Agric Food Chem 2008; 56(16): 7032-7. [CrossRef] google scholar
  • 11. Hsu DZ, Chen KT, Li YH, Chuang YC, Liu MY. Sesamol delays mortality and attenuates hepatic injury after cecal ligation and puncture in rats: role of oxidative stress. Shock. 2006; 25(5): 52832. [CrossRef] google scholar
  • 12. Hsu DZ, Chen YW, Chu PY, Periasamy S, Liu MY. Protective effect of 3,4-methylenedioxyphenol (sesamol) on stress-related mucosal disease in rats. Biomed Res Int 2013; 2013: 481827. google scholar
  • 13. Kumagai Y, Lin LY, Schmitz DA, Cho AK. Hydroxyl radical mediated demethylenation of (methylenedioxy) phenyl compounds. Chem Res Toxicol 1991; 4: 330-4. [CrossRef] google scholar
  • 14. Kanimozhi P, Prasad NR. Antioxidant potential of sesamol and its role on radiation-induced DNA damage in whole-body irradiated Swiss albino mice. Environ Toxicol Pharmacol 2009; 28: 192-7. [CrossRef] google scholar
  • 15. Prasad NR, Menon VP, Vasudev V, Pugalendi KV. Radioprotective effect of sesamol on gamma-radiation induced DNA damage, lipid peroxidation and antioxidants levels in cultured human lymphocytes. Toxicology 2005; 209: 225-35. [CrossRef] google scholar
  • 16. Wu MS, Aquino LBB, Barbaza MYU, Hsieh CL, Castro-Cruz KA, Yang LL, et al. Anti-inflammatory and anticancer properties of bioactive compounds from Sesamum indicum L.-A Review. Molecules 2019; 24(24): 4426. [CrossRef] google scholar
  • 17. Hsu D-Z, Chu P-Y, Li Y-H, Liu M-Y. Sesamol attenuates diclofenac-induced acute gastric mucosal injury via its cyclooxygenaseindependent antioxidative effect in rats. Shock 2008; 30(4): 45662. [CrossRef] google scholar
  • 18. Zargar HR, Hemmati AA, Ghafourian M, Arzi A, Rezaie A, Javad-Moosavi SA. Long-term treatment with royal jelly improves bleomycin-induced pulmonary fibrosis in rats. Can J Physiol Pharmacol 2017; 95(1): 23-31. [CrossRef] google scholar
  • 19. Javadi I, Emami S. The antioxidative effect of chamomile, anthocyanoside and their combination on bleomycin-induced pulmonary fibrosis in rat. Med Arch 2015; 69(4): 229-31. [CrossRef] google scholar
  • 20. Yang D, Yuan W, Lv C, Li N, Liu T, Wang L, et al. Dihydroartemisinin supresses inflammation and fibrosis in bleomycine-induced pulmonary fibrosis in rats. Int J Clin Exp Pathol 2015; 8(2): 1270-81. google scholar
  • 21. Akgedik R, Akgedik S, Karamanli H, Uysal S, Bozkurt B, Ozol D, et al. Effect of resveratrol on treatment of bleomycin-induced pulmonary fibrosis in rats. Inflammation 2012; 35(5): 1732-41. [CrossRef] google scholar
  • 22. Jan KC, Ho CT, Hwang LS. Elimination and metabolism of sesamol, a bioactive compound in sesame oil, in rats. Mol Nutr Food Res 2009; 53(1): 36-43. [CrossRef] google scholar
  • 23. Shruthi P, Ajay KC, Mamta S, Mangal S, Ravi M. Correlation of histopathological diagnosis with habits and clinical findings in oral submucous fibrosis. Head Neck Oncol 2009; 1-10. [CrossRef] google scholar
  • 24. Kawai K, Akaza H. Bleomycin-induced pulmonary toxicity in chemotherapy for testicular cancer. Expert Opin Drug Saf 2003; 2 (6): 587-96. [CrossRef] google scholar
  • 25. Lee WJ, Lee SM, Won CH, Chang SE, Lee MW, Choi JH, et al. Efficacy of intralesional bleomycin for the treatment of plantar hard corns. Int J Dermatol 2014; 53(12): 572-7. [CrossRef] google scholar
  • 26. Utsunomiya H, Tilakaratne WM, Oshiro K, Satoshi Maruyama, Makoto Suzuki, Hiroko Ida-Yonemochi, et al. Extracellular matrix remodeling in oral submucous fibrosis: its stage-specific modes revealed by immunohistochemistry and in situ hybridization. J Oral Pathol Med 2005; 34: 498-507. [CrossRef] google scholar
  • 27. Dai JP, Chen XX, Zhu DX, Wan QY, Chen C, Wang GF, et al. Panax notoginseng saponins inhibit areca nut extract induced oral submucous fibrosis in vitro. J Oral Pathol Med 2014; 43(6): 464-70. [CrossRef] google scholar
  • 28. Jiang L, Deng Y, Li W, Lu Y. Arctigenin suppresses fibroblast activity and extracellular matrix deposition in hypertrophic scarring by reducing inflammation and oxidative stress. Mol Med Rep 2020; 22(6): 4783-91. [CrossRef] google scholar
  • 29. Andargie M, Vinas M, Rathgeb A, Möller E, Karlovsky P. Lignans of Sesame (Sesamum indicum L.): A Comprehensive Review. Molecules 2021; 26(4): 883. [CrossRef] google scholar
Yıl 2022, Cilt: 12 Sayı: 3, 238 - 242, 31.12.2022
https://doi.org/10.26650/experimed.1195246

Öz

Kaynakça

  • 1. World Health Organization; International Agency for Research on Cancer. IARC monograph on the evaluation of carcinogenic risks to humans, betel-quid and areca-nut chewing and some arecanut-derived nitrosamines. Vol. 85. Lyon, France: IARC; 2004. google scholar
  • 2. Arakeri G, Brennan PA. Oral submucous fibrosis: An overview of the aetiology, pathogenesis, classification, and principles of management. Br J Oral Maxillofac Surg 2013; 51: 587-93. [CrossRef] google scholar
  • 3. Angadi PV, Kale AD, Hallikerimath S. Evaluation of myofibroblasts in oral submucous fibrosis: correlation with disease severity. J Oral Pathol Med 2011; 40: 208-13. [CrossRef] google scholar
  • 4. Kerr AR, Warnakulasuriya S, Mighell AJ, T Dietrich, M Nasser, J Rimal, et al. A systematic review of medical interventions for oral submucous fibrosis and future research opportunities. Oral Dis 2011; 17: 42-57. [CrossRef] google scholar
  • 5. Jiang XW, Zhang Y, Yang SK, Zhang H, Lu K, Sun GL. Efficacy of salvianolic acid B combined with triamcinolone acetonide in the treatment of oral submucous fibrosis. Oral Surg Oral Med Oral Pathol Oral Radiol 2013; 115: 339-44. [CrossRef] google scholar
  • 6. Chua F, Gauldie J, Laurent GJ. Pulmonary fibrosis: Searching for model answers. Am J Respir Cell Mol Biol 2005; 33: 9-13. [CrossRef] google scholar
  • 7. Yamamoto T, Takagawa S, Katayama I, Yamazaki K, Hamazaki Y, Shinkai H, et al. Animal model of sclerotic skin. I: Local injections of bleomycin induce sclerotic skin mimicking scleroderma. J Invest Dermatol 1999; 112: 456-62. [CrossRef] google scholar
  • 8- Zhang SS, Gong ZJ, Xiong W, Wang X, Min Q, Luo CD, et al. A rat model of oral submucous fibrosis induced by bleomycin. Oral Surg Oral Med Oral Pathol Oral Radiol 2016; 122(2): 216-23. [CrossRef] google scholar
  • 9- Kabel AM, Omar MS, Elmaaboud MAA. Amelioration of bleomycin-induced lung fibrosis in rats by valproic acid and butyrate: Role of nuclear factor kappa-B, proinflammatory cytokines and oxidative stress. Int Immunopharmacol 2016; 39: 335-42. [CrossRef] google scholar
  • 10. Jan KC, Ho CT, Hwang LS. Bioavailability and tissue distribution of sesamol in rat. J Agric Food Chem 2008; 56(16): 7032-7. [CrossRef] google scholar
  • 11. Hsu DZ, Chen KT, Li YH, Chuang YC, Liu MY. Sesamol delays mortality and attenuates hepatic injury after cecal ligation and puncture in rats: role of oxidative stress. Shock. 2006; 25(5): 52832. [CrossRef] google scholar
  • 12. Hsu DZ, Chen YW, Chu PY, Periasamy S, Liu MY. Protective effect of 3,4-methylenedioxyphenol (sesamol) on stress-related mucosal disease in rats. Biomed Res Int 2013; 2013: 481827. google scholar
  • 13. Kumagai Y, Lin LY, Schmitz DA, Cho AK. Hydroxyl radical mediated demethylenation of (methylenedioxy) phenyl compounds. Chem Res Toxicol 1991; 4: 330-4. [CrossRef] google scholar
  • 14. Kanimozhi P, Prasad NR. Antioxidant potential of sesamol and its role on radiation-induced DNA damage in whole-body irradiated Swiss albino mice. Environ Toxicol Pharmacol 2009; 28: 192-7. [CrossRef] google scholar
  • 15. Prasad NR, Menon VP, Vasudev V, Pugalendi KV. Radioprotective effect of sesamol on gamma-radiation induced DNA damage, lipid peroxidation and antioxidants levels in cultured human lymphocytes. Toxicology 2005; 209: 225-35. [CrossRef] google scholar
  • 16. Wu MS, Aquino LBB, Barbaza MYU, Hsieh CL, Castro-Cruz KA, Yang LL, et al. Anti-inflammatory and anticancer properties of bioactive compounds from Sesamum indicum L.-A Review. Molecules 2019; 24(24): 4426. [CrossRef] google scholar
  • 17. Hsu D-Z, Chu P-Y, Li Y-H, Liu M-Y. Sesamol attenuates diclofenac-induced acute gastric mucosal injury via its cyclooxygenaseindependent antioxidative effect in rats. Shock 2008; 30(4): 45662. [CrossRef] google scholar
  • 18. Zargar HR, Hemmati AA, Ghafourian M, Arzi A, Rezaie A, Javad-Moosavi SA. Long-term treatment with royal jelly improves bleomycin-induced pulmonary fibrosis in rats. Can J Physiol Pharmacol 2017; 95(1): 23-31. [CrossRef] google scholar
  • 19. Javadi I, Emami S. The antioxidative effect of chamomile, anthocyanoside and their combination on bleomycin-induced pulmonary fibrosis in rat. Med Arch 2015; 69(4): 229-31. [CrossRef] google scholar
  • 20. Yang D, Yuan W, Lv C, Li N, Liu T, Wang L, et al. Dihydroartemisinin supresses inflammation and fibrosis in bleomycine-induced pulmonary fibrosis in rats. Int J Clin Exp Pathol 2015; 8(2): 1270-81. google scholar
  • 21. Akgedik R, Akgedik S, Karamanli H, Uysal S, Bozkurt B, Ozol D, et al. Effect of resveratrol on treatment of bleomycin-induced pulmonary fibrosis in rats. Inflammation 2012; 35(5): 1732-41. [CrossRef] google scholar
  • 22. Jan KC, Ho CT, Hwang LS. Elimination and metabolism of sesamol, a bioactive compound in sesame oil, in rats. Mol Nutr Food Res 2009; 53(1): 36-43. [CrossRef] google scholar
  • 23. Shruthi P, Ajay KC, Mamta S, Mangal S, Ravi M. Correlation of histopathological diagnosis with habits and clinical findings in oral submucous fibrosis. Head Neck Oncol 2009; 1-10. [CrossRef] google scholar
  • 24. Kawai K, Akaza H. Bleomycin-induced pulmonary toxicity in chemotherapy for testicular cancer. Expert Opin Drug Saf 2003; 2 (6): 587-96. [CrossRef] google scholar
  • 25. Lee WJ, Lee SM, Won CH, Chang SE, Lee MW, Choi JH, et al. Efficacy of intralesional bleomycin for the treatment of plantar hard corns. Int J Dermatol 2014; 53(12): 572-7. [CrossRef] google scholar
  • 26. Utsunomiya H, Tilakaratne WM, Oshiro K, Satoshi Maruyama, Makoto Suzuki, Hiroko Ida-Yonemochi, et al. Extracellular matrix remodeling in oral submucous fibrosis: its stage-specific modes revealed by immunohistochemistry and in situ hybridization. J Oral Pathol Med 2005; 34: 498-507. [CrossRef] google scholar
  • 27. Dai JP, Chen XX, Zhu DX, Wan QY, Chen C, Wang GF, et al. Panax notoginseng saponins inhibit areca nut extract induced oral submucous fibrosis in vitro. J Oral Pathol Med 2014; 43(6): 464-70. [CrossRef] google scholar
  • 28. Jiang L, Deng Y, Li W, Lu Y. Arctigenin suppresses fibroblast activity and extracellular matrix deposition in hypertrophic scarring by reducing inflammation and oxidative stress. Mol Med Rep 2020; 22(6): 4783-91. [CrossRef] google scholar
  • 29. Andargie M, Vinas M, Rathgeb A, Möller E, Karlovsky P. Lignans of Sesame (Sesamum indicum L.): A Comprehensive Review. Molecules 2021; 26(4): 883. [CrossRef] google scholar
Toplam 29 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Klinik Tıp Bilimleri
Bölüm Araştırma Makalesi
Yazarlar

Sevilay Erimşah 0000-0001-5400-0012

Ayhan Çetinkaya 0000-0002-8212-7149

Ümmügül Üyetürk 0000-0002-6839-2632

Uğur Üyetürk 0000-0002-4313-8478

Yusufhan Yazır 0000-0002-8472-0261

Yayımlanma Tarihi 31 Aralık 2022
Gönderilme Tarihi 27 Ekim 2022
Yayımlandığı Sayı Yıl 2022 Cilt: 12 Sayı: 3

Kaynak Göster

Vancouver Erimşah S, Çetinkaya A, Üyetürk Ü, Üyetürk U, Yazır Y. The Antioxidant Effects of Sesamol on Bleomycin-Induced Oral Submucous Fibrosis. Experimed. 2022;12(3):238-42.