The Effect of Short-Chain Fatty Acids On Epithelial Repair In Experimental Colitis Created With Trinitrobenzenesulfonic Acid In Rats
Abstract
Objectives: The purpose of this study is to discuss short-chain fatty acids’ SCFA role in ulcerative colitis UC treatment by using rats which have experimental colitis created with trinitrobenzenesulfonic acid TNBS . Method: In this study, the experimental research method was adopted. The study group consisted of 30 female Wistar rats which have 150±30 gr. average weights, Rats were randomly divided into 3 groups with 10 rats in each group. The groups were randomly assigned as two experimental groups and one control group. The first experimental group was administered SCFA intracolonically 2 times a day from the 3rd day, and the second experimental group was administered SCFA 2 times a day from the first day. The control group was given saline 2 times a day from the 3rd day. On day 6, the rats were decapitated. MPO activity evaluation and macroscopic and microscopic evaluation of the colon mucosa was done. Results: There was a statistically significant difference between the colon macroscopy results of the groups 1-2, 2-3, 1-3 p
References
- Selamawit T, Georgia C, Dhima, E, Houston CG, Augenlicht L, & Velcich A. MUC2 mucin deficiency alters inflammatory and metabolic pathways in the mouse intestinal mucosa. Oncotarget 2017;8:71456–70. [CrossRef]
- Ungaro R, Mehandru S, Allen P B, Peyrin-Biroulet L, Colombel JF. Ulcerative colitis. The Lancet 2017;389:1756–70. [CrossRef]
- Rhodes J, Thomas G. Mucosal protective and repair agents in the treatment of colitis. In: Bayless TM, Hanauer SB, editors. Advanced Therapy of Inflammatory Bowel Disease. London: B.C. Decker Inc.; 2001. pp. 107–10.
- ands BE. Novel therapies for inflammatory bowel disease. Gastroenterol Clin North Am 1999;28:323–51.
- Aguilar-Nascimento JE, França-da-Silva LR, de Oliveria AF, Gomes- da-Silva MH. Enhanced mucosal reepithelialization induced by short chain fatty acids in experimental colitis. Braz J Med 1999;32:961–6. [CrossRef]
- Andres PG, Friedman LS. Epidemiology and the natural course of inflamatory bowel disease. Gastroenterol Clin North Am 1999;28:255–81. [CrossRef]
- Mi H, Liu FB, Li HW, Hou JT, Li PW. Anti-inflammatory effect of Chang-An-Shuan on TNBS-induced experimental colitis in rats. BMC 2017;17:315–22. [CrossRef]
- Maltz RM, Keirsey J, Kim SC, Mackos AR, Gharaibeh RZ, Moore CC, Xu J, et al. Prolonged restraint stressor exposure in outbred CD-1 mice impacts microbiota, colonic inflammation, and short chain fatty acids. PLoS One 2018;13:e0196961. [CrossRef]
- Roediger WE, Nance S. Selective reduction of fatty acid oxidation in colonocytes correlation with ulcerative colitis. Lipids 1990;25:646– 52. [CrossRef]
- Harig JM, Soergel KH, Komorowski RA, Wood CM. Treatment of diversion colitis with short chain fatty acid irrigation. New Engl J Med 1989;320:23–8. [CrossRef]
- von Herbay A, Gebbers JO, Otto HF. Immunopathology of ulcerative colitis. Hepatogastroenterology 1990;37:99–107.
- Warren BF, Shepherd NA, Bartolo DC, Bradfneld JW. Pathology of defunctioned rectum in ulcerative colitis. Gut 1993;34:514–6. [CrossRef]
- Gibson PR, Barkla DH. Mucosal metabolism and proliferation. In: Inflammatory Bowel Disease UK: Churchill Livingstone; 1997. pp.201–11.
- Polyviou T, MacDougall K, Chambers ES, Viardot A, Psichas A, Jawaid S, et al. Randomised clinical study: inulin short‐chain fatty acid esters for targeted delivery of short‐chain fatty acids to the human colon. Aliment Pharmacol Ther 2016;44:662–72. [CrossRef]
- Senyelli B. İntraperitoneal mitomisin C ile geliştirilen deneysel kolitte antioksidanların etkisi (Uzmanlık tezi). Edirne: Trakya Üniversitesi Tıp Fakültesi; 1999.
- Papadakis KA, Targan SR. Current theories on the causes of inflammatory bowel disease. Gastroenterol Clin North Am 1999;28:283–95. [CrossRef]
- Rachmilewitz D, Stamler JS, Karmeli F, Mullins ME, Singel DJ, Loscalzo J, et al. Peroxynitrite induced rat colitis, a new model of colonic inflammation. Gastroenterology 1993;105:1681–8. [CrossRef]
- Geraghty JM, Talbot IC. Diversion colitis histological features in the colon and rectum after defunctioning colostomy. Gut 1991;32:1020– 3. [CrossRef]
- Elson CO, Sartor RB, Tennyson GS, Riddell RH. Experimental models of inflammatory bowel disease. Gastroenterology 1995;109:1344– 67. [CrossRef]
- Guimbaud R, Bertrand V, Chauvelot-Moachon L, Quartier G, Vidon N, Giroud JP, et al. Network of inflammatory cytokines and correlation with disease activity in ulcerative colitis. Am J Gastroenterol 1998;93:2397–404. [CrossRef]
- Mayer L. Current concepts of inflammatory bowel disease etiology and pathogenesis. In: Kirsner JB, editor. Inflammatory Bowel Disease, 5th ed. Philadelphia, PA: WB Saunders Co.; 2000. pp.280–96.
- Wolfgang S, German-Austrian Scfa Study Group. Treatment of distal ulcerative colitis with short chain fatty acid enemas. Dig Dis and Sci 1996;41:2254–9. [CrossRef]
- Primec M, Mičetić-Turk D, Langerholc T. Analysis of short-chain fatty acids in human feces: A scoping review. Anal Biochem 2017;526:9– 21. [CrossRef]
- Stumpff F. A look at the smelly side of physiology: transport of short chain fatty acids. Pflügers Archiv - Eur J Physiol 2018;470:571–98. [CrossRef]
- Çağlar A, Tomar O, Ekiz, T. Bütirik asit: yapısı, özellikleri ve sağlık üzerine etkileri. Kocatepe Vet J 2017;10:213–25. [CrossRef]
- Köseler E. Ülseratif kolitte nutrisyon. Güncel Gastroenteroloji 2016;20:263–6. http://guncel.tgv.org.tr/journal/67/pdf/100478.pdf
- Corrêa‐Oliveira R, Fachi JL, Vieira A, Sato FT, Vinolo MA. Regulation of immune cell function by short‐chain fatty acids. Clin Transl Immunology 2016;5:e73. [CrossRef]
- Miner PB. Clinical features, course, laboratory findings and complications in ulcerative colitis. In: Kirsner JB, editor. Inflammatory Bowel Disease, 5th ed. Philadelphia, PA: WB Saunders Co.; 2000. pp.299–304.
- Butzner JD, Parmar R, Bell CJ, Dalal V. Butyrate enema therapy stimulates mucosal repair in experimental colitis in the rat. Gut 1996;38:568–73. [CrossRef]
Ratlarda Trinitrobenzensulfonik Asit ile Oluşturulan Deneysel Kolitte Kısa Zincirli Yağ Asitlerinin Epitel Onarımına Etkisi
Abstract
Amaç: Bu araştırmanın amacı, trinitrobenzensulfonikasid TNBS ile deneysel kolit oluşturulan ratları kullanarak kısa zincirli yağ asitlerinin ülseratif kolit tedavisindeki yerini tartışmaktır. Yöntem: Bu araştırmada, deneysel araştırma yöntemi kullanılmıştır. Ortalama ağırlıkları 150±30 gr. arasında değişen 30 dişi Wistar cinsi rat çalışma grubunu oluşturmuştur. Ratlar randomize olarak 10’ar adetlik üç gruba ayrılmıştır. Gruplar, iki grup deney, bir grup kontrol grubu olmak üzere yine randomize olarak atanmıştır. Birinci deney grubuna 3. günden itibaren günde iki kez intrakolonik olarak KZYA, ikinci deney grubuna ilk günden itibaren günde iki kez KZYA olarak uygulanmıştır. Kontrol grubuna ise 3. günden itibaren günde iki kez serum fizyolojik uygulanmıştır. 6. günde çalışma sonlandırılarak ratlar dekapite edilmiştir. Kolon mukozasının makroskopik ve mikroskobik değerlendirmesiyle MPO aktivitesi değerlendirmesi yapılmıştır. Bulgular: 1.-2., 2.-3., 1.-3. grupların kolon makroskopileri arasında istatistiksel olarak anlamlı fark bulunmuştur p
References
- Selamawit T, Georgia C, Dhima, E, Houston CG, Augenlicht L, & Velcich A. MUC2 mucin deficiency alters inflammatory and metabolic pathways in the mouse intestinal mucosa. Oncotarget 2017;8:71456–70. [CrossRef]
- Ungaro R, Mehandru S, Allen P B, Peyrin-Biroulet L, Colombel JF. Ulcerative colitis. The Lancet 2017;389:1756–70. [CrossRef]
- Rhodes J, Thomas G. Mucosal protective and repair agents in the treatment of colitis. In: Bayless TM, Hanauer SB, editors. Advanced Therapy of Inflammatory Bowel Disease. London: B.C. Decker Inc.; 2001. pp. 107–10.
- ands BE. Novel therapies for inflammatory bowel disease. Gastroenterol Clin North Am 1999;28:323–51.
- Aguilar-Nascimento JE, França-da-Silva LR, de Oliveria AF, Gomes- da-Silva MH. Enhanced mucosal reepithelialization induced by short chain fatty acids in experimental colitis. Braz J Med 1999;32:961–6. [CrossRef]
- Andres PG, Friedman LS. Epidemiology and the natural course of inflamatory bowel disease. Gastroenterol Clin North Am 1999;28:255–81. [CrossRef]
- Mi H, Liu FB, Li HW, Hou JT, Li PW. Anti-inflammatory effect of Chang-An-Shuan on TNBS-induced experimental colitis in rats. BMC 2017;17:315–22. [CrossRef]
- Maltz RM, Keirsey J, Kim SC, Mackos AR, Gharaibeh RZ, Moore CC, Xu J, et al. Prolonged restraint stressor exposure in outbred CD-1 mice impacts microbiota, colonic inflammation, and short chain fatty acids. PLoS One 2018;13:e0196961. [CrossRef]
- Roediger WE, Nance S. Selective reduction of fatty acid oxidation in colonocytes correlation with ulcerative colitis. Lipids 1990;25:646– 52. [CrossRef]
- Harig JM, Soergel KH, Komorowski RA, Wood CM. Treatment of diversion colitis with short chain fatty acid irrigation. New Engl J Med 1989;320:23–8. [CrossRef]
- von Herbay A, Gebbers JO, Otto HF. Immunopathology of ulcerative colitis. Hepatogastroenterology 1990;37:99–107.
- Warren BF, Shepherd NA, Bartolo DC, Bradfneld JW. Pathology of defunctioned rectum in ulcerative colitis. Gut 1993;34:514–6. [CrossRef]
- Gibson PR, Barkla DH. Mucosal metabolism and proliferation. In: Inflammatory Bowel Disease UK: Churchill Livingstone; 1997. pp.201–11.
- Polyviou T, MacDougall K, Chambers ES, Viardot A, Psichas A, Jawaid S, et al. Randomised clinical study: inulin short‐chain fatty acid esters for targeted delivery of short‐chain fatty acids to the human colon. Aliment Pharmacol Ther 2016;44:662–72. [CrossRef]
- Senyelli B. İntraperitoneal mitomisin C ile geliştirilen deneysel kolitte antioksidanların etkisi (Uzmanlık tezi). Edirne: Trakya Üniversitesi Tıp Fakültesi; 1999.
- Papadakis KA, Targan SR. Current theories on the causes of inflammatory bowel disease. Gastroenterol Clin North Am 1999;28:283–95. [CrossRef]
- Rachmilewitz D, Stamler JS, Karmeli F, Mullins ME, Singel DJ, Loscalzo J, et al. Peroxynitrite induced rat colitis, a new model of colonic inflammation. Gastroenterology 1993;105:1681–8. [CrossRef]
- Geraghty JM, Talbot IC. Diversion colitis histological features in the colon and rectum after defunctioning colostomy. Gut 1991;32:1020– 3. [CrossRef]
- Elson CO, Sartor RB, Tennyson GS, Riddell RH. Experimental models of inflammatory bowel disease. Gastroenterology 1995;109:1344– 67. [CrossRef]
- Guimbaud R, Bertrand V, Chauvelot-Moachon L, Quartier G, Vidon N, Giroud JP, et al. Network of inflammatory cytokines and correlation with disease activity in ulcerative colitis. Am J Gastroenterol 1998;93:2397–404. [CrossRef]
- Mayer L. Current concepts of inflammatory bowel disease etiology and pathogenesis. In: Kirsner JB, editor. Inflammatory Bowel Disease, 5th ed. Philadelphia, PA: WB Saunders Co.; 2000. pp.280–96.
- Wolfgang S, German-Austrian Scfa Study Group. Treatment of distal ulcerative colitis with short chain fatty acid enemas. Dig Dis and Sci 1996;41:2254–9. [CrossRef]
- Primec M, Mičetić-Turk D, Langerholc T. Analysis of short-chain fatty acids in human feces: A scoping review. Anal Biochem 2017;526:9– 21. [CrossRef]
- Stumpff F. A look at the smelly side of physiology: transport of short chain fatty acids. Pflügers Archiv - Eur J Physiol 2018;470:571–98. [CrossRef]
- Çağlar A, Tomar O, Ekiz, T. Bütirik asit: yapısı, özellikleri ve sağlık üzerine etkileri. Kocatepe Vet J 2017;10:213–25. [CrossRef]
- Köseler E. Ülseratif kolitte nutrisyon. Güncel Gastroenteroloji 2016;20:263–6. http://guncel.tgv.org.tr/journal/67/pdf/100478.pdf
- Corrêa‐Oliveira R, Fachi JL, Vieira A, Sato FT, Vinolo MA. Regulation of immune cell function by short‐chain fatty acids. Clin Transl Immunology 2016;5:e73. [CrossRef]
- Miner PB. Clinical features, course, laboratory findings and complications in ulcerative colitis. In: Kirsner JB, editor. Inflammatory Bowel Disease, 5th ed. Philadelphia, PA: WB Saunders Co.; 2000. pp.299–304.
- Butzner JD, Parmar R, Bell CJ, Dalal V. Butyrate enema therapy stimulates mucosal repair in experimental colitis in the rat. Gut 1996;38:568–73. [CrossRef]
There are 29 citations in total.
Details
| Primary Language | Turkish |
|---|---|
| Journal Section | Research Article |
| Authors | |
| Publication Date | December 1, 2020 |
| Published in Issue | Year 2020Issue: 4 |