Araştırma Makalesi
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Aşırı Kilolu Kişilerde Olası Genotoksik Hasarın Comet Deneyi ile Analizi

Yıl 2022, Cilt: 17 Sayı: 3, 43 - 48, 02.11.2022
https://doi.org/10.17517/ksutfd.942657

Öz

Özet
Amaç: Son yıllarda yapılmış olan araştırmalar ile obezitenin, DNA zincir kırıklarının onarım mekanizmasını değiştirdiği ortaya çıkarılmıştır. Ayrıca beden kitle indeksinde artış ile genomik kararsızlık arasında ilişki tespit edilmiştir. Sunulan çalışmada aşırı kilolu bireylerin muhtemel genotoksik hasarının araştırılması amaçlanmış olup periferal kan örneklerinde Tek Hücre Jel Elektroforezi deneyi kullanılarak olası genotoksik hasar düzeyi hesaplanmıştır.
Gereç ve Yöntemler: Sunulan çalışmada 18 yaşından büyük aşırı kilolu ya da normal kiloya sahip bireylerin periferal lenfositlerinde olası DNA hasarı Comet Deneyi ile analiz edilmiştir. Sonuçlar SPSS analiz programı kullanılarak istatistiksel olarak karşılaştırılmıştır.
Bulgular: Sunulan araştırmaya yaş ortalaması 30.13±7.97 olan 23 kadın ve yaş ortalaması 38.13±10.63 olan 32 erkek (21 normal kilolu ve 34 aşırı kilolu) toplamda 55 gönüllü katılmıştır. DNA hasarının göstergesi olan kuyruk momenti değeri tüm bireylerde ortalama 1.21±0.45’dir. Aşırı kilolu kişilerin kuyruk momenti değeri ortalama 1.29±0.46 olarak bulunmuştur. Bu değer normal kiloya sahip bireylerin kuyruk momenti sonuçları (1.09±0.40) ile karşılaştırıldığında istatistiksel olarak anlamlı derecede fark bulunmamıştır (p>0.05). Çalışma sonuçlarımıza göre beden kitle indeksinde artış ile DNA hasarı arasında anlamlı
fark bulunmamıştır (p>0.05).
Sonuç: Sunulan çalışma Türkiye’deki yetişkin bireylerde aşırı kiloluluk ve DNA hasar düzeyinin değerlendirildiği ilk çalışma olma özelliğindedir. Sunulan çalışmada Comet Deneyi bulgularına göre beden kitle indeksi ve genotoksik hasar arasında ilişki bulunmadığı tespit edilmiş olup gelecekte DNA hasar düzeyinin farklı genotoksisite testleriyle araştırılacağı yeni çalışmaların yapılması önerilmektedir.

Kaynakça

  • World Health Organization. Health Topics Obesity. https://www.who.int/topics/obesity/en/.
  • Wlodarczyk M, Jablonowska-Lietz B, Olejarz W, Nowicka G. Anthropometric and dietary factors as predictors of DNA damage in obese women. Nutrients 2018;10:578.
  • Zaki M, Basha W, El Bassyouni, El-Toukhy S, Hussein T. Evaluation of DNA damage profile in obese women and its association to risk of metabolic syndrome, polycystic ovary syndrome and recurrent preeclampsia. Genes Dis 2018;5:367–373.
  • Włodarczyk M, Nowicka G. Obesity, DNA damage and development of obesity-related diseases. Int J Mol Sci 2019;20:1146.
  • Bukhari SA, Rajoka MI, Ibrahim Z, Jalal F, Rana SM, Nagra SA. Oxidative stress elevated DNA damage and homocysteine level in normal pregnant women in a segment of Pakistani population. Mol Biol Rep 2011;38:2703–2710.
  • Sancar A. Excision repair in mammalian cells. J Biol Chem 1995;270:15915–15918.
  • Yuzefovych LV, Musiyenko SI, Wilson GL, Rachek LI. Mitochondrial DNA damage and dysfunction, and oxidative stress are associated with endoplasmic reticulum stress, protein degradation and apoptosis in high fat diet-induced insulin resistance mice. PLoS ONE 2013;8:54059.
  • Pazmandi K, Agod Z, Kumar BV, Szabo A, Fekete T, Sogor V et al. Oxidative modification enhances the immunostimulatory effects of extracellular mitochondrial DNA on plasmacytoid dendritic cells. Free Radic Biol Med 2014;77:281–290.
  • Tyson J, Spiers A, Caple F, Hesketh JE, Mathers JC. Effects of age, body mass index and genotype on nucleotide excision repair in healthy adults. P Nutr Soc 2006;114.
  • Azzarà A, Pirillo C, Giovannini C, Federico G, Scarpato R. Different repair kinetic of DSBs induced by mitomycin C in peripheral lymphocytes of obese and normal weight adolescents. Mutat Res Fund Mol M. 2016;789:9-14.
  • Wolin KY, Carson K, Colditz GA. Obesity and cancer. The oncologist. 2010;15(6):556.
  • Avgerinos KI, Spyrou N, Mantzoros CS, Dalamaga M. Obesity and cancer risk: Emerging biological mechanisms and perspectives. Metabolism 2019;92:121-135.
  • Kyrgiou M, Kalliala I, Markozannes G, Gunter MJ, Paraskevaidis E, Gabra H et al. Adiposity and cancer at major anatomical sites: umbrella review of the literature. BMJ. 2017;356:477.
  • Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H et al. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 2000;35:206-221.
  • Gandhi G, Kaur G. Assessment of DNA damage in obese individuals. Res J Biol 2012;2:37-44.
  • Setayesh T, Nersesyan A, Mišík M, Ferk F, Langie S, Andrade VM, et al. Impact of obesity and overweight on DNA stability: Few facts and many hypotheses. Mutat Res Rev Mutat 2018;777:64-91.
  • Zaki ME, El-Bassyouni HT, Yousef W, Mohamed R, El Toukhy S, Ismail S. Body image, anxiety, depression and DNA damage in obese Egyptian women. Middle East J Med Genet 2019;8:42.
  • Santovito A, Gendusa C. Micronuclei frequency in peripheral blood lymphocytes of healthy subjects living in turin (North-Italy): Contribution of body mass index, age and sex. Ann Hum Biol 2020;47:48-54.
  • Franzke B, Schwingshackl L, Wagner KH. Chromosomal damage measured by the cytokinesis block micronucleus cytome assay in diabetes and obesity-A systematic review and meta-analysis. Mutat Res Rev Mutat Res 2020;108343.
  • Evenocheck A, Rhee Y, Hall, C. Do Health promoting compounds of flaxseed attenuate weight gain via modulation of obesity gene expression? Plant Foods Hum Nut 2020;75:441-445.
  • Tomasello B, Malfa G, Galvano F, Renis M. DNA damage in normal-weight obese syndrome measured by Comet assay. Mediterr J Nutr Metab 2011;2:99–104.
  • Scarpato R, Verola C, Fabiani B, Bianchi V, Saggese G, Federico G. Nuclear damage in peripheral lymphocytes of obese and overweight Italian children as evaluated by the gamma-H2AX focus assay and micronucleus test. FASEB J 2011;25:685–693.
  • Dupont C, Faure C, Sermondade N, Boubaya M, Eustache F, Clément P et al. Obesity leads to higher risk of sperm DNA damage in infertile patients. J Androl 2013;15:622.
  • Dönmez-Altuntas H, Sahin F, Bayram F, Bitgen N, Mert M, Guclu K et al. Evaluation of chromosomal damage, cytostasis, cytotoxicity, oxidative DNA damage and their association with body-mass index in obese subjects. Mutat Res Gen Tox En 2014;771:30-36.
  • Cerdá, C, Sánchez C, Climent B, Vázquez A, Iradi A, El Amrani F et al. Oxidative stress and DNA damage in obesity-related tumorigenesis. Adv Exp Med Biol 2014;824:5-17.
  • Milić M, Kišan M, Rogulj D, Radman M, Lovrenčić MV, Konjevoda P et al. Level of primary DNA damage in the early stage of metabolic syndrome. Mutat Res Gen Tox En 2013;758:1-5.

Investigation of Genotoxic Damage in Overweight Individuals Using the Comet Assay

Yıl 2022, Cilt: 17 Sayı: 3, 43 - 48, 02.11.2022
https://doi.org/10.17517/ksutfd.942657

Öz

Abstract
Objective: Research has revealed that obesity changed the repair mechanism of DNA chain breaks. Also, the increase in body mass index is found out to be associated with genomic instability. In this study, it was aimed to investigate the possible genotoxic damage of overweight individuals.
Material and Methods: In the present study the level of genotoxic damage was calculated in Turkish adult peripheral blood samples using Single Cell Gel Electrophoresis assay. The results of possible DNA damage levels belonging to overweight people were compared statistically by SPSS analysis program with the results of normal-weight people.
Results: Fifty five volunteers (21 normal weight and 34 overweight); 23 women, mean age=30.13±7.97 and 32 men, mean age=38.13±10.63 participated in the study. Tail moment is an average of 1.21±0.45 in all individuals. Tail moment value was found of overweight people as 1.29±0.46. When this value was compared with the results of individuals with normal weight (1.09±0.40), statistically no significant difference was determined (p>0.05). According to results, no significant difference was found between the increase in body mass index and DNA damage (p>0.05).
Conclusion: The present study is the first study which gives information about the level of DNA damage relation with being overweight in adults of Turkey. In the presented study, it was determined that there was no relationship between body mass index and genotoxic damage according to the findings of the comet assay, and we recommended that new studies should be conducted in the future to investigate the level of DNA damage with different genotoxicity tests.

Kaynakça

  • World Health Organization. Health Topics Obesity. https://www.who.int/topics/obesity/en/.
  • Wlodarczyk M, Jablonowska-Lietz B, Olejarz W, Nowicka G. Anthropometric and dietary factors as predictors of DNA damage in obese women. Nutrients 2018;10:578.
  • Zaki M, Basha W, El Bassyouni, El-Toukhy S, Hussein T. Evaluation of DNA damage profile in obese women and its association to risk of metabolic syndrome, polycystic ovary syndrome and recurrent preeclampsia. Genes Dis 2018;5:367–373.
  • Włodarczyk M, Nowicka G. Obesity, DNA damage and development of obesity-related diseases. Int J Mol Sci 2019;20:1146.
  • Bukhari SA, Rajoka MI, Ibrahim Z, Jalal F, Rana SM, Nagra SA. Oxidative stress elevated DNA damage and homocysteine level in normal pregnant women in a segment of Pakistani population. Mol Biol Rep 2011;38:2703–2710.
  • Sancar A. Excision repair in mammalian cells. J Biol Chem 1995;270:15915–15918.
  • Yuzefovych LV, Musiyenko SI, Wilson GL, Rachek LI. Mitochondrial DNA damage and dysfunction, and oxidative stress are associated with endoplasmic reticulum stress, protein degradation and apoptosis in high fat diet-induced insulin resistance mice. PLoS ONE 2013;8:54059.
  • Pazmandi K, Agod Z, Kumar BV, Szabo A, Fekete T, Sogor V et al. Oxidative modification enhances the immunostimulatory effects of extracellular mitochondrial DNA on plasmacytoid dendritic cells. Free Radic Biol Med 2014;77:281–290.
  • Tyson J, Spiers A, Caple F, Hesketh JE, Mathers JC. Effects of age, body mass index and genotype on nucleotide excision repair in healthy adults. P Nutr Soc 2006;114.
  • Azzarà A, Pirillo C, Giovannini C, Federico G, Scarpato R. Different repair kinetic of DSBs induced by mitomycin C in peripheral lymphocytes of obese and normal weight adolescents. Mutat Res Fund Mol M. 2016;789:9-14.
  • Wolin KY, Carson K, Colditz GA. Obesity and cancer. The oncologist. 2010;15(6):556.
  • Avgerinos KI, Spyrou N, Mantzoros CS, Dalamaga M. Obesity and cancer risk: Emerging biological mechanisms and perspectives. Metabolism 2019;92:121-135.
  • Kyrgiou M, Kalliala I, Markozannes G, Gunter MJ, Paraskevaidis E, Gabra H et al. Adiposity and cancer at major anatomical sites: umbrella review of the literature. BMJ. 2017;356:477.
  • Tice RR, Agurell E, Anderson D, Burlinson B, Hartmann A, Kobayashi H et al. Single cell gel/comet assay: guidelines for in vitro and in vivo genetic toxicology testing. Environ Mol Mutagen 2000;35:206-221.
  • Gandhi G, Kaur G. Assessment of DNA damage in obese individuals. Res J Biol 2012;2:37-44.
  • Setayesh T, Nersesyan A, Mišík M, Ferk F, Langie S, Andrade VM, et al. Impact of obesity and overweight on DNA stability: Few facts and many hypotheses. Mutat Res Rev Mutat 2018;777:64-91.
  • Zaki ME, El-Bassyouni HT, Yousef W, Mohamed R, El Toukhy S, Ismail S. Body image, anxiety, depression and DNA damage in obese Egyptian women. Middle East J Med Genet 2019;8:42.
  • Santovito A, Gendusa C. Micronuclei frequency in peripheral blood lymphocytes of healthy subjects living in turin (North-Italy): Contribution of body mass index, age and sex. Ann Hum Biol 2020;47:48-54.
  • Franzke B, Schwingshackl L, Wagner KH. Chromosomal damage measured by the cytokinesis block micronucleus cytome assay in diabetes and obesity-A systematic review and meta-analysis. Mutat Res Rev Mutat Res 2020;108343.
  • Evenocheck A, Rhee Y, Hall, C. Do Health promoting compounds of flaxseed attenuate weight gain via modulation of obesity gene expression? Plant Foods Hum Nut 2020;75:441-445.
  • Tomasello B, Malfa G, Galvano F, Renis M. DNA damage in normal-weight obese syndrome measured by Comet assay. Mediterr J Nutr Metab 2011;2:99–104.
  • Scarpato R, Verola C, Fabiani B, Bianchi V, Saggese G, Federico G. Nuclear damage in peripheral lymphocytes of obese and overweight Italian children as evaluated by the gamma-H2AX focus assay and micronucleus test. FASEB J 2011;25:685–693.
  • Dupont C, Faure C, Sermondade N, Boubaya M, Eustache F, Clément P et al. Obesity leads to higher risk of sperm DNA damage in infertile patients. J Androl 2013;15:622.
  • Dönmez-Altuntas H, Sahin F, Bayram F, Bitgen N, Mert M, Guclu K et al. Evaluation of chromosomal damage, cytostasis, cytotoxicity, oxidative DNA damage and their association with body-mass index in obese subjects. Mutat Res Gen Tox En 2014;771:30-36.
  • Cerdá, C, Sánchez C, Climent B, Vázquez A, Iradi A, El Amrani F et al. Oxidative stress and DNA damage in obesity-related tumorigenesis. Adv Exp Med Biol 2014;824:5-17.
  • Milić M, Kišan M, Rogulj D, Radman M, Lovrenčić MV, Konjevoda P et al. Level of primary DNA damage in the early stage of metabolic syndrome. Mutat Res Gen Tox En 2013;758:1-5.
Toplam 26 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Sağlık Kurumları Yönetimi
Bölüm Araştırma Makaleleri
Yazarlar

Gülşen Göney 0000-0002-5236-1241

Muhammed Oğuz Halisdemir 0000-0003-0548-7463

Erken Görünüm Tarihi 1 Kasım 2022
Yayımlanma Tarihi 2 Kasım 2022
Gönderilme Tarihi 25 Mayıs 2021
Kabul Tarihi 17 Ağustos 2021
Yayımlandığı Sayı Yıl 2022 Cilt: 17 Sayı: 3

Kaynak Göster

AMA Göney G, Halisdemir MO. Investigation of Genotoxic Damage in Overweight Individuals Using the Comet Assay. KSÜ Tıp Fak Der. Kasım 2022;17(3):43-48. doi:10.17517/ksutfd.942657