Öz
Kaynakça
- 1- Rausch V, Seybold D, Königshausen M, Köller M, Schildhauer TA, Geßmann J. Basic principles of fracture healing. Orthopade. 2017 Aug;46(8):640-7
- 2- Zura R, Xiong Z, Einhorn T, Watson JT, Ostrum RF, Prayson MJ, Della Rocca GJ, Mehta S, McKinley T, Wang Z, Steen RG. Epidemiology of fracture nonunion in 18 human bones. JAMA Surg. 2016 Nov 16; 151(11): e162775
- 3- Kerimoglu S, Livaoglu M, Sonmez B, Yulug E, Aynacı O, Topbas M, Yarar S. Effects of Human Amniotic Fluid on Fracture Healing in Rat Tibia. J. of Surg Res 2008: 152, 281-7
- 4- Dahl LB, Kimpton WG, Cahill RNP, Brown TJ, Fraser JRE. The origin and fate of hyaluronan in amniotic fluid. J Dev Physiol. 1989; 12:209-18
- 5- Longaker MT, Adzick NS, Hall JL, Stair SE, Crombleholme TM, Duncan BW, Bradley SM, Harrison MR, Stern R: Studies in fetal wound healing, VII. Fetal wound healing may be modulated by hyaluronic acid stimulating activity in amniotic fluid. J Pediatr Surg. 1990 Apr;25(4):430-3.
- 6- Longaker MT, Adzick NS: The biology of fetal wound healing: a review. Plast Reconstr Surg. 1991 Apr;87(4):788-98.
- 7- Longaker MT, Chiu ES, Harrison MR, Crombleholme TM, Langer JC, Duncan BW,Adzick NS, Verrier ED, Stern R: Studies in fetal wound healing. IV. Hyaluronic acid-stimulating activity distinguishes fetal wound fluid from adult wound fluid. Ann Surg. 1989 Nov;210(5):667-72.
- 8- Huang L, Cheng YY, Koo PL, Lee KM, Qin L, Cheng JC, Kumta SM. The effect of hyaluronan on osteoblast proliferation and differentiation in rat calvarial-derived cell cultures.Journal of Biomedical Materials Research 2003;66(4):880-84
- 9- Ozgenel GY, Samli B, Ozcan M: Effects of human amniotic fluid on peritendinous adhesion formation and tendon healing after flexor tendon surgery in rabbits. J Hand Surg [Am]. 2001 Mar;26(2):332-9
- 10- Ozgenel GY, Filiz G: Combined application of human amniotic membrane wrapping and hyaluronic acid injection in epineurectomized rat sciatic nerve. J Reconstr Microsurg. 2004 Feb;20(2):153-7.
- 11- Ozgenel GY: The effects of a combination of hyaluronic and amniotic membrane on the formation of peritendinous adhesions after flexor tendon surgery in chickens. J Bone Joint Surg Br. 2004 Mar;86(2):301-7
- 12- Tenenhaus M. The Use of Human Amnion/Chorion Membranes in the Treatment of Burns and Complex Wounds. Annals of Plastic Surgery: 78, S11-S13
- 13- Tejwani S, Kolari RS, Sangwan VS, Rao GN:Role of amniotic membrane graft for ocular chemical and thermal injuries. Cornea 2007;26:21-26
- 14- Lee H, Ha SW, Kim JC:A novel application of amniotic membrane in patients with bullous keratopathy. J Korean Med Sci.2006;21:324-28
- 15- Simpson AHRW,Mills L, Noble B:The role of growth factors and related agents in accelerating fracture healing. J Bone Joint Surg 2006;88B(6):701-05
- 16- Ozgenel GY, Filiz G: Effects of human amniotic fluid on peripheral nerve scarring and regeneration in rats. J Neurosurg. 2003 Feb;98(2):371-7
- 17- Ozgenel GY: The influence of human amniotic fluid on the potential of rabbit ear perichondrial flaps to form cartilage tissue. Br J Plast Surg. 2002 Apr;55(3):246-50.
- 18- Heiple KG, Goldberg VM, Powell AE, Bos GD, Zika JM:Biology of cancellous bone grafts.Orthop Clin North Am. 1987;18:179-85
- 19- Aslan M, Şimşek G,Dayı E:The effect of hyaluronic acid-supplemented bone graft in bone healing:Experimental study in rabbits.Journal of Biomaterials Applications.2006;20:209-20
- 20- Radomsky ML, Aufdemorte TB,Swain LD,Fox CW: Novel formulation of fibroblast growth factor-2 and hyaluronan gel accelerates fracture healing in nonhuman primates. J Orthop Res. 1999;17:607-14
- 21- Eckardt H, Christensen KS,Lind M, Hansen ES, Hall DW, Hvid I. Recombinant human bone morphogenetic protein-2 enhances bone healing in an experimental model of fractures at risk of nonunion. Injury.2005;36:489-94
- 22- Vishwakarma GK, Khare AK: Amniotic arthroplasty for tuberculosis of the hip. A preliminary clinical study. J Bone Joint Surg Br. 1986 Jan;68(1):68-74
- 23- Nunamaker DM:Experimental models of fracture repair. Clin Orthop Relat Res. 1998 ;355Supp:56-65
- 24- Mencucci R, Menchini U, Dei R:Antimicrobial activity of antibiotic-treated amniotic membrane:An in vitro study. Cornea 2006;25:428-31
- 25- Burgos H: Angiogenic and growth factors in human amnio-chorion and placenta.Eur J Clin Invest. 1983;13(4):289-96
- 26- Jackson RA, McDonald MM, Nurcombe V,Little DG,Cool SM:The use of heparan sulfate to augment fracture repair in a rat fracture model. J Orthop Res. 2006;Apr:636-44
- 27- Utvag SE, Grundnes O, Reikeras O:Effects of lesion between bone,periosteum and muscle on fracture healing in rats. Acta Orthop Scand. 1998;69(2):177-80
Öz
Purpose:Human amniotic fluid contains several growth factors and hyaluronic acid. It has positive effect in healing the tendon, nerve and bone injuries. Human amniotic membrane has positive effects on tendon, nerve healing and is also used for skin healing in burns and complex wounds. We hypothesized that human amniotic fluid and human amniotic membrane have positive stimulating effects on fracture healing. The aim of this study is to investigate the effects of human amniotic fluid and human amniotic membrane on bone healing in rat tibial bone fracture model.
Materials and Methods:A 72 open fracture model was created for both tibial bones of 36 male Wistar rats. They were divided into three groups. Group-1 was the control group. In Group-2, human amniotic fluid which was taken from amniocentesis of seronegative pregnants in their second trimester was injected to the fracture site. In group-3, human amniotic membrane which was taken from normal seronegative term pregnants after their caesarean sections was wrapped around fracture site and then human amniotic fluid was injected to the fracture site. Radiological fracture union patterns and histopathological evaluations with Modified Lane-Sandhu classification were performed with Modified Lane-Sandhu classification at the end of third and sixth weeks.
Results:At third week, Group-2 and Group-3 had better histopathological results than Group-1. At sixth week, Group-2 had significantly better results than Group-1. Group-3 had also better results than Group-1 although there was no statistical significance. At third and sixth weeks, histopathological scores of Group-2 were significantly better than Group-3(p=0.021, p=0.019).
Conclusion:Our data suggested that human amniotic fluid had positive effect while human amniotic membrane had no positive effect on bone healing. With its low immunity and easy delivery, human amniotic membrane can also be used to stimulate bone healing in combination with human amniotic fluid with different application techniques.
Anahtar Kelimeler
Tibia fracture model human amniotic fluid human amniotic membrane animal study
Kaynakça
- 1- Rausch V, Seybold D, Königshausen M, Köller M, Schildhauer TA, Geßmann J. Basic principles of fracture healing. Orthopade. 2017 Aug;46(8):640-7
- 2- Zura R, Xiong Z, Einhorn T, Watson JT, Ostrum RF, Prayson MJ, Della Rocca GJ, Mehta S, McKinley T, Wang Z, Steen RG. Epidemiology of fracture nonunion in 18 human bones. JAMA Surg. 2016 Nov 16; 151(11): e162775
- 3- Kerimoglu S, Livaoglu M, Sonmez B, Yulug E, Aynacı O, Topbas M, Yarar S. Effects of Human Amniotic Fluid on Fracture Healing in Rat Tibia. J. of Surg Res 2008: 152, 281-7
- 4- Dahl LB, Kimpton WG, Cahill RNP, Brown TJ, Fraser JRE. The origin and fate of hyaluronan in amniotic fluid. J Dev Physiol. 1989; 12:209-18
- 5- Longaker MT, Adzick NS, Hall JL, Stair SE, Crombleholme TM, Duncan BW, Bradley SM, Harrison MR, Stern R: Studies in fetal wound healing, VII. Fetal wound healing may be modulated by hyaluronic acid stimulating activity in amniotic fluid. J Pediatr Surg. 1990 Apr;25(4):430-3.
- 6- Longaker MT, Adzick NS: The biology of fetal wound healing: a review. Plast Reconstr Surg. 1991 Apr;87(4):788-98.
- 7- Longaker MT, Chiu ES, Harrison MR, Crombleholme TM, Langer JC, Duncan BW,Adzick NS, Verrier ED, Stern R: Studies in fetal wound healing. IV. Hyaluronic acid-stimulating activity distinguishes fetal wound fluid from adult wound fluid. Ann Surg. 1989 Nov;210(5):667-72.
- 8- Huang L, Cheng YY, Koo PL, Lee KM, Qin L, Cheng JC, Kumta SM. The effect of hyaluronan on osteoblast proliferation and differentiation in rat calvarial-derived cell cultures.Journal of Biomedical Materials Research 2003;66(4):880-84
- 9- Ozgenel GY, Samli B, Ozcan M: Effects of human amniotic fluid on peritendinous adhesion formation and tendon healing after flexor tendon surgery in rabbits. J Hand Surg [Am]. 2001 Mar;26(2):332-9
- 10- Ozgenel GY, Filiz G: Combined application of human amniotic membrane wrapping and hyaluronic acid injection in epineurectomized rat sciatic nerve. J Reconstr Microsurg. 2004 Feb;20(2):153-7.
- 11- Ozgenel GY: The effects of a combination of hyaluronic and amniotic membrane on the formation of peritendinous adhesions after flexor tendon surgery in chickens. J Bone Joint Surg Br. 2004 Mar;86(2):301-7
- 12- Tenenhaus M. The Use of Human Amnion/Chorion Membranes in the Treatment of Burns and Complex Wounds. Annals of Plastic Surgery: 78, S11-S13
- 13- Tejwani S, Kolari RS, Sangwan VS, Rao GN:Role of amniotic membrane graft for ocular chemical and thermal injuries. Cornea 2007;26:21-26
- 14- Lee H, Ha SW, Kim JC:A novel application of amniotic membrane in patients with bullous keratopathy. J Korean Med Sci.2006;21:324-28
- 15- Simpson AHRW,Mills L, Noble B:The role of growth factors and related agents in accelerating fracture healing. J Bone Joint Surg 2006;88B(6):701-05
- 16- Ozgenel GY, Filiz G: Effects of human amniotic fluid on peripheral nerve scarring and regeneration in rats. J Neurosurg. 2003 Feb;98(2):371-7
- 17- Ozgenel GY: The influence of human amniotic fluid on the potential of rabbit ear perichondrial flaps to form cartilage tissue. Br J Plast Surg. 2002 Apr;55(3):246-50.
- 18- Heiple KG, Goldberg VM, Powell AE, Bos GD, Zika JM:Biology of cancellous bone grafts.Orthop Clin North Am. 1987;18:179-85
- 19- Aslan M, Şimşek G,Dayı E:The effect of hyaluronic acid-supplemented bone graft in bone healing:Experimental study in rabbits.Journal of Biomaterials Applications.2006;20:209-20
- 20- Radomsky ML, Aufdemorte TB,Swain LD,Fox CW: Novel formulation of fibroblast growth factor-2 and hyaluronan gel accelerates fracture healing in nonhuman primates. J Orthop Res. 1999;17:607-14
- 21- Eckardt H, Christensen KS,Lind M, Hansen ES, Hall DW, Hvid I. Recombinant human bone morphogenetic protein-2 enhances bone healing in an experimental model of fractures at risk of nonunion. Injury.2005;36:489-94
- 22- Vishwakarma GK, Khare AK: Amniotic arthroplasty for tuberculosis of the hip. A preliminary clinical study. J Bone Joint Surg Br. 1986 Jan;68(1):68-74
- 23- Nunamaker DM:Experimental models of fracture repair. Clin Orthop Relat Res. 1998 ;355Supp:56-65
- 24- Mencucci R, Menchini U, Dei R:Antimicrobial activity of antibiotic-treated amniotic membrane:An in vitro study. Cornea 2006;25:428-31
- 25- Burgos H: Angiogenic and growth factors in human amnio-chorion and placenta.Eur J Clin Invest. 1983;13(4):289-96
- 26- Jackson RA, McDonald MM, Nurcombe V,Little DG,Cool SM:The use of heparan sulfate to augment fracture repair in a rat fracture model. J Orthop Res. 2006;Apr:636-44
- 27- Utvag SE, Grundnes O, Reikeras O:Effects of lesion between bone,periosteum and muscle on fracture healing in rats. Acta Orthop Scand. 1998;69(2):177-80
Ayrıntılar
| Birincil Dil | İngilizce |
|---|---|
| Konular | Klinik Tıp Bilimleri |
| Bölüm | Araştırma Makaleleri |
| Yazarlar | |
| Yayımlanma Tarihi | 1 Mart 2020 |
| Gönderilme Tarihi | 11 Şubat 2019 |
| Kabul Tarihi | 15 Nisan 2019 |
| Yayımlandığı Sayı | Yıl 2020 Cilt: 27 Sayı: 1 |
Süleyman Demirel Üniversitesi Tıp Fakültesi Dergisi/Medical Journal of Süleyman Demirel University is licensed under Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International.