Research Article
Year 2023,
, 488 - 494, 01.10.2023
Abstract
Background: Human colostrum, the first form of milk produced by mammary glands, is crucial for newborn development. Nowadays, there is a great interest in finding alternative colostrum from different species to replace the extremely scarce human colostrum. In this study, we investigated the proteomic profiles of whey fractions of human and goat colostrum samples to understand the proteomic differences and gain insight into the potential functions of these proteins.
Methods: Proteomic profiles of human (n=6) and goat (n=6) colostrum that were collected at the early stages of lactation were investigated using two-dimensional difference gel electrophoresis (DIGE). Spot intensity differences were detected and spots were identified by MALDI-TOF/TOF mass spectrometry. Functional annotation analyses were performed.
Results: In total, 533 spots were detected and identified in human and goat colostrum samples. Immunoglobulin, casein, lactoferrin, lactoglobulin, albumin, lactotransferrin, and lactalbumin proteins were found to be abundant. Low abundance proteins such as α1-antitrypsin, cathelicidin, galectin-3-binding protein, lactadherin, tenascin, and apolipoprotein J were also detected. Functional annotation analysis showed that human colostrum proteins were commonly involved in the phagosome, complement and coagulation pathways, and disease-related pathways.
Conclusion: Our results provide a preliminary proteomic comparison between human and goat colostrum samples. The proteins detected in the whey fractions of human and goat colostrum showed a remarkable number of common proteins. Moreover, human colostrum showed disease-related pathway enrichments and further suggests the role of passive immunization that might protect the newborn from diseases.
Supporting Institution
This work was funded in part by short term scientific mission granted by COST 20 Action (FA1002) on Farm Animal Proteomics.
References
- Hettinga K, van Valenberg H, de Vries S, et al (2011) The host defense proteome of human and bovine milk. PLoS One 6:e19433. https://doi.org/10.1371/journal.pone.0019433
- Lönnerdal B (2016) Bioactive Proteins in Human Milk: Health, Nutrition, and Implications for Infant Formulas. J Pediatr 173 Suppl:S4-9. https://doi.org/10.1016/j.jpeds.2016.02.070
- Hurley WL, Theil PK (2011) Perspectives on immunoglobulins in colostrum and milk. Nutrients 3:442–474. https://doi.org/10.3390/nu3040442
- Mangé A, Bellet V, Tuaillon E, et al (2008) Comprehensive proteomic analysis of the human milk proteome: Contribution of protein fractionation. J Chromatogr B Anal Technol Biomed Life Sci 876:252–256. https://doi.org/10.1016/j.jchromb.2008.11.003
- Roncada P, Piras C, Soggiu A, et al (2012) Farm animal milk proteomics. J Proteomics 75:4259–4274. https://doi.org/10.1016/j.jprot.2012.05.028
- Stelwagen K, Carpenter E, Haigh B, et al (2009) Immune components of bovine colostrum and milk. J Anim Sci 87:3–9. https://doi.org/10.2527/jas.2008-1377
- Lifschitz C, Szajewska H (2015) Cow’s milk allergy: evidence-based diagnosis and management for the practitioner. Eur J Pediatr 174:141–150. https://doi.org/10.1007/s00431-014-2422-3
- Park YW (1994) Hypo-allergenic and therapeutic significance of goat milk. Small Rumin Res 14:151–159. https://doi.org/https://doi.org/10.1016/0921-4488(94)90105-8
- Lönnerdal B (2003) Nutritional and physiologic significance of human milk proteins. Am J Clin Nutr 77:. https://doi.org/10.1093/ajcn/77.6.1537s
- Senda A, Fukuda K, Ishii T, Urashima T (2011) Changes in the bovine whey proteome during the early lactation period. Anim Sci J 82:698–706. https://doi.org/10.1111/j.1740-0929.2011.00886.x
- Pereira PC (2014) Milk nutritional composition and its role in human health. Nutrition 30:619–627. https://doi.org/10.1016/j.nut.2013.10.011
- D’Alessandro A, Scaloni A, Zolla L (2010) Human milk proteins: An interactomics and updated functional overview. J Proteome Res 9:3339–3373. https://doi.org/10.1021/pr100123f
- Sun Y, Wang C, Sun X, Guo M (2020) Proteomic analysis of whey proteins in the colostrum and mature milk of Xinong Saanen goats. J Dairy Sci 103:1164–1174. https://doi.org/https://doi.org/10.3168/jds.2019-17159
- Sun Y, Wang C, Sun X, et al (2020) Characterization of the milk fat globule membrane proteome in colostrum and mature milk of Xinong Saanen goats. J Dairy Sci 103:3017–3024. https://doi.org/https://doi.org/10.3168/jds.2019-17739
- Liao Y, Alvarado R, Phinney B, Lönnerdal B (2011) Proteomic characterization of specific minor proteins in the human milk casein fraction. J Proteome Res 10:5409–5415. https://doi.org/10.1021/pr200660t
- Nissen A, Bendixen E, Ingvartsen KL, Røntved CM (2012) In-depth analysis of low abundant proteins in bovine colostrum using different fractionation techniques. Proteomics 12:2866–2878. https://doi.org/10.1002/pmic.201200231
- Palmer DJ, Kelly VC, Smit AM, et al (2006) Human colostrum: Identification of minor proteins in the aqueous phase by proteomics. Proteomics 6:2208–2216. https://doi.org/10.1002/pmic.200500558
- Yang M, Deng W, Cao X, et al (2020) Quantitative Phosphoproteomics of Milk Fat Globule Membrane in Human Colostrum and Mature Milk: New Insights into Changes in Protein Phosphorylation during Lactation. J Agric Food Chem 68:4546–4556. https://doi.org/10.1021/acs.jafc.9b06850
- Yang M, Cao X, Wu R, et al (2017) Comparative proteomic exploration of whey proteins in human and bovine colostrum and mature milk using iTRAQ-coupled LC-MS/MS. Int J Food Sci Nutr 68:671–681. https://doi.org/10.1080/09637486.2017.1279129
- Taylor FB, Hack E, Lupu F (2006) Observations on Complement Activity in the Two-Stage Inflammatory/Hemostatic Response in the Baboon and Human Models of E. Coli Sepsis and Endotoxemia BT - Current Topics in Complement. In: Lambris JD (ed). Springer US, Boston, MA, pp 203–216
- Mendonça M, Moreira GMSG, Conceição FR, et al (2016) Fructose 1,6-Bisphosphate Aldolase, a Novel Immunogenic Surface Protein on Listeria Species. PLoS One 11:e0160544–e0160544. https://doi.org/10.1371/journal.pone.0160544
- Kessler EC, Bruckmaier RM, Gross JJ (2019) Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany. J Dairy Sci 102:5542–5549. https://doi.org/https://doi.org/10.3168/jds.2018-16235
- Charlwood J, Hanrahan S, Tyldesley R, et al (2002) Use of proteomic methodology for the characterization of human milk fat globular membrane proteins. Anal Biochem 301:314–324. https://doi.org/10.1006/abio.2001.5498
- Ah-Leung S, Bernard H, Bidat E, et al (2006) Allergy to goat and sheep milk without allergy to cow’s milk. Allergy 61:1358–1365. https://doi.org/10.1111/j.1398-9995.2006.01193.x
- (2012) Scientific Opinion on the suitability of goat milk protein as a source of protein in infant formulae and in follow-on formulae. EFSA J 10:2603. https://doi.org/10.2903/j.efsa.2012.2603
- Hodgkinson AJ, McDonald NA, Kivits LJ, et al (2012) Allergic responses induced by goat milk αS1-casein in a murine model of gastrointestinal atopy. J Dairy Sci 95:83–90. https://doi.org/10.3168/jds.2011-4829
- Tay EP, Gam LH (2011) Proteomics of human and the domestic bovine and caprine milk. Asia-Pacific J Mol Biol Biotechnol 19:45–53
- Artym J, Zimecki M (2013) Milk-derived proteins and peptides in clinical trials. Postepy Hig Med Dosw 67:800–816. https://doi.org/10.5604/17322693.1061635
Year 2023,
, 488 - 494, 01.10.2023
Abstract
References
- Hettinga K, van Valenberg H, de Vries S, et al (2011) The host defense proteome of human and bovine milk. PLoS One 6:e19433. https://doi.org/10.1371/journal.pone.0019433
- Lönnerdal B (2016) Bioactive Proteins in Human Milk: Health, Nutrition, and Implications for Infant Formulas. J Pediatr 173 Suppl:S4-9. https://doi.org/10.1016/j.jpeds.2016.02.070
- Hurley WL, Theil PK (2011) Perspectives on immunoglobulins in colostrum and milk. Nutrients 3:442–474. https://doi.org/10.3390/nu3040442
- Mangé A, Bellet V, Tuaillon E, et al (2008) Comprehensive proteomic analysis of the human milk proteome: Contribution of protein fractionation. J Chromatogr B Anal Technol Biomed Life Sci 876:252–256. https://doi.org/10.1016/j.jchromb.2008.11.003
- Roncada P, Piras C, Soggiu A, et al (2012) Farm animal milk proteomics. J Proteomics 75:4259–4274. https://doi.org/10.1016/j.jprot.2012.05.028
- Stelwagen K, Carpenter E, Haigh B, et al (2009) Immune components of bovine colostrum and milk. J Anim Sci 87:3–9. https://doi.org/10.2527/jas.2008-1377
- Lifschitz C, Szajewska H (2015) Cow’s milk allergy: evidence-based diagnosis and management for the practitioner. Eur J Pediatr 174:141–150. https://doi.org/10.1007/s00431-014-2422-3
- Park YW (1994) Hypo-allergenic and therapeutic significance of goat milk. Small Rumin Res 14:151–159. https://doi.org/https://doi.org/10.1016/0921-4488(94)90105-8
- Lönnerdal B (2003) Nutritional and physiologic significance of human milk proteins. Am J Clin Nutr 77:. https://doi.org/10.1093/ajcn/77.6.1537s
- Senda A, Fukuda K, Ishii T, Urashima T (2011) Changes in the bovine whey proteome during the early lactation period. Anim Sci J 82:698–706. https://doi.org/10.1111/j.1740-0929.2011.00886.x
- Pereira PC (2014) Milk nutritional composition and its role in human health. Nutrition 30:619–627. https://doi.org/10.1016/j.nut.2013.10.011
- D’Alessandro A, Scaloni A, Zolla L (2010) Human milk proteins: An interactomics and updated functional overview. J Proteome Res 9:3339–3373. https://doi.org/10.1021/pr100123f
- Sun Y, Wang C, Sun X, Guo M (2020) Proteomic analysis of whey proteins in the colostrum and mature milk of Xinong Saanen goats. J Dairy Sci 103:1164–1174. https://doi.org/https://doi.org/10.3168/jds.2019-17159
- Sun Y, Wang C, Sun X, et al (2020) Characterization of the milk fat globule membrane proteome in colostrum and mature milk of Xinong Saanen goats. J Dairy Sci 103:3017–3024. https://doi.org/https://doi.org/10.3168/jds.2019-17739
- Liao Y, Alvarado R, Phinney B, Lönnerdal B (2011) Proteomic characterization of specific minor proteins in the human milk casein fraction. J Proteome Res 10:5409–5415. https://doi.org/10.1021/pr200660t
- Nissen A, Bendixen E, Ingvartsen KL, Røntved CM (2012) In-depth analysis of low abundant proteins in bovine colostrum using different fractionation techniques. Proteomics 12:2866–2878. https://doi.org/10.1002/pmic.201200231
- Palmer DJ, Kelly VC, Smit AM, et al (2006) Human colostrum: Identification of minor proteins in the aqueous phase by proteomics. Proteomics 6:2208–2216. https://doi.org/10.1002/pmic.200500558
- Yang M, Deng W, Cao X, et al (2020) Quantitative Phosphoproteomics of Milk Fat Globule Membrane in Human Colostrum and Mature Milk: New Insights into Changes in Protein Phosphorylation during Lactation. J Agric Food Chem 68:4546–4556. https://doi.org/10.1021/acs.jafc.9b06850
- Yang M, Cao X, Wu R, et al (2017) Comparative proteomic exploration of whey proteins in human and bovine colostrum and mature milk using iTRAQ-coupled LC-MS/MS. Int J Food Sci Nutr 68:671–681. https://doi.org/10.1080/09637486.2017.1279129
- Taylor FB, Hack E, Lupu F (2006) Observations on Complement Activity in the Two-Stage Inflammatory/Hemostatic Response in the Baboon and Human Models of E. Coli Sepsis and Endotoxemia BT - Current Topics in Complement. In: Lambris JD (ed). Springer US, Boston, MA, pp 203–216
- Mendonça M, Moreira GMSG, Conceição FR, et al (2016) Fructose 1,6-Bisphosphate Aldolase, a Novel Immunogenic Surface Protein on Listeria Species. PLoS One 11:e0160544–e0160544. https://doi.org/10.1371/journal.pone.0160544
- Kessler EC, Bruckmaier RM, Gross JJ (2019) Immunoglobulin G content and colostrum composition of different goat and sheep breeds in Switzerland and Germany. J Dairy Sci 102:5542–5549. https://doi.org/https://doi.org/10.3168/jds.2018-16235
- Charlwood J, Hanrahan S, Tyldesley R, et al (2002) Use of proteomic methodology for the characterization of human milk fat globular membrane proteins. Anal Biochem 301:314–324. https://doi.org/10.1006/abio.2001.5498
- Ah-Leung S, Bernard H, Bidat E, et al (2006) Allergy to goat and sheep milk without allergy to cow’s milk. Allergy 61:1358–1365. https://doi.org/10.1111/j.1398-9995.2006.01193.x
- (2012) Scientific Opinion on the suitability of goat milk protein as a source of protein in infant formulae and in follow-on formulae. EFSA J 10:2603. https://doi.org/10.2903/j.efsa.2012.2603
- Hodgkinson AJ, McDonald NA, Kivits LJ, et al (2012) Allergic responses induced by goat milk αS1-casein in a murine model of gastrointestinal atopy. J Dairy Sci 95:83–90. https://doi.org/10.3168/jds.2011-4829
- Tay EP, Gam LH (2011) Proteomics of human and the domestic bovine and caprine milk. Asia-Pacific J Mol Biol Biotechnol 19:45–53
- Artym J, Zimecki M (2013) Milk-derived proteins and peptides in clinical trials. Postepy Hig Med Dosw 67:800–816. https://doi.org/10.5604/17322693.1061635
There are 28 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Research Article |
| Authors | |
| Early Pub Date | September 4, 2023 |
| Publication Date | October 1, 2023 |
| Submission Date | April 5, 2023 |
| Published in Issue | Year 2023 |