Flow Cytometric Analysis of Lymphocyte Subsets of Covid-19 Patients from A Single Centre in Turkey

Tuğrul Elverdi; Abdülkadir Erçalışkan; Rıdvan Karaali; İlker İnanç Balkan; Deniz Özmen; Ayşe Salihoğlu; Birgül Mete; Ahmet Emre Eşkazan; Zafer Başlar; Fehmi Tabak; Muhlis Cem Ar

doi:10.31067/acusaglik.1335196

EN

Flow Cytometric Analysis of Lymphocyte Subsets of Covid-19 Patients from A Single Centre in Turkey

Abstract

Purpose: Lymphocyte counts have been shown to negatively correlate with the severity in Covid-19. The aim of this study is to analyse the distribution of lymphocyte subsets in response to SARS-CoV-2 infection and its relation to the severity of the disease. Methods: Blood samples were obtained from 67 consecutive patients between April 2020 and July 2020. Data on other laboratory parameters, and clinical course were collected retrospectively from patient files and patients were defined to have as severe or non-severe (mild/moderate) disease. Leukocyte subsets to be studied were identified by using flow cytometric analysis (Beckman Coulter Navios Ex V2.0). Patients were allocated into 3 groups based on the day of blood sample collection: Days 0-7, 8-14 and >14 as Group I, Group II and Group III, respectively. In 10 of 67 patients an additional analysis was done 7-10 days after the initial sampling. Results: A total of 67 patients (30 female, 37 male) with a median age of 57 were evaluated. Lower total lymphocyte, CD3 positive, CD4 positive and B-cell counts were identified in severe infection compared to non-severe infection group which were also correlated with high serum CRP, D-dimer and ferritin levels. NK and monocyte counts were not different between the two groups. Activation markers CD38 and HLA-DR on CD4 and CD8 positive lymphocytes also were not different in either group. Conclusion: CD3 and CD4 lymphopenia were lower in accordance with previous studies and were associated with severe disease. The expectancy of high activation markers was not met. Future studies with detailed subgroup analyses at different time-points will shed more light on our general knowledge of the immune response to COVID-19.

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Details

Primary Language

English

Subjects

Haematology

Journal Section

Research Article

Authors

Tuğrul Elverdi ^*
0000-0001-9496-5353
Türkiye

Abdülkadir Erçalışkan
0000-0002-1795-5773
Türkiye

Rıdvan Karaali
0000-0003-2440-7529
Türkiye

İlker İnanç Balkan
0000-0002-8977-5931
Türkiye

Deniz Özmen
0000-0001-7561-446X
Türkiye

Ayşe Salihoğlu
0000-0002-8758-7945
Türkiye

Birgül Mete
0000-0001-9417-8640
Türkiye

Ahmet Emre Eşkazan
0000-0001-9568-0894
Türkiye

Zafer Başlar
0000-0002-1807-7351
Türkiye

Fehmi Tabak
0000-0001-8632-2825
Türkiye

Muhlis Cem Ar
0000-0002-0332-9253
Türkiye

Early Pub Date

September 20, 2023

Publication Date

October 1, 2023

Submission Date

August 1, 2023

Acceptance Date

September 10, 2023

Published in Issue

Year 1970 Volume: 14 Number: 4

DOI

https://doi.org/10.31067/acusaglik.1335196

IZ

https://izlik.org/JA76ZN22MY

Cite

RIS / Bibtex

EndNote

Elverdi T, Erçalışkan A, Karaali R, Balkan İİ, Özmen D, Salihoğlu A, Mete B, Eşkazan AE, Başlar Z, Tabak F, Ar MC (October 1, 2023) Flow Cytometric Analysis of Lymphocyte Subsets of Covid-19 Patients from A Single Centre in Turkey. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi 14 4 534–541.