Nikotinin Sıçan Pankreatit Modelindeki Hafifletici Etkisinde Vagusun Rolünün Araştırılması

Meltem Kolgazi; Zozan Güleken; Bircan Kolbaşı; Canberk Sami Başıbüyük; Feriha Ercan; Berrak Yeğen

doi:10.31067/acusaglik.849995

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Nikotinin Sıçan Pankreatit Modelindeki Hafifletici Etkisinde Vagusun Rolünün Araştırılması

Öz

Nikotininbirçok farklı dokuda anti-inflamatuvar etkilere sahip olduğu ve bu etkileri kolinerjik anti-inflamatuvar yolağı aktive ederek gerçekleştirdiği gösterilmiştir. Çalışmada akut pankreatit modelinde nikotin tedavisinin etkilerini ve bu etkilerin kolinerjik yolla ilişkisini araştırmak amaçlanmıştır. Wistar albino sıçanların pankreas-safra ortak kanalı bağlanırken(PSKB),bir grubada yalancı-cerrahi (YC)uygulandı. PSKB sıçanlaravagal aferent denervasyon(perivagal kapsaisin; 10mg/ml)veya trunkal vagotomi uygulandı ya da vagusları sağlam bırakıldı. PSKB grupları cerrahi sonrası ikiye ayrılarak 4 gün boyunca intraperitoneal nikotin (1 mg/kg/gün) ya da serum fizyolojik verildi. Dördüncü günde dekapitasyonu takiben,serumda tümör nekroz faktör (TNF)- ve interlökin (IL)-10 ölçümü, akciğer, karaciğer ve pankreas dokularındamiyeloperoksidaz aktivitesi (MPO), malondialdehit (MDA), glutatyon (GSH) ölçümleri ve histolojik inceleme yapıldı. TNF- düzeyi PSKB grubunda YC grubuna göre belirgin şekilde daha yüksekken, nikotin tedavisi alanlarda belirgin şekilde daha düşüktü. IL-10 düzeyi ise SF tedavisi almış PSKB grubunda YC grubuna göre daha düşüktü ve nikotin tedavisi ile arttığı gözlendi.YC grubuna göre PSKB grubunun pankreas, karaciğer ve akciğer dokularında mikroskopik hasar, MDA, MPO düzeyleri artarken GSH düzeyleri azaldı. Nikotin tedavisi ile karaciğer ve pankreas dokularındaki hasar, MDA, MPO düzeyleri azaldı ve GSH miktarları korundu. Vagal aferent denervasyon ya da trunkal vagotomi yapılması nikotinin bu koruyucu etkilerini değiştirmedi. Sonuçlar, nikotininpankreatit hasarına karşıkoruyucu etkilerini vagal yoldan bağımsız olarak, doğrudan immün hücreler üzerindeki 7nAChR aktivasyonu ile nötrofil infiltrasyonunu ve pro-inflamatuvar sitokinleri inhibe ederek gerçekleştirdiğini ortaya koymaktadır.

Anahtar Kelimeler

Investigation of The Role of Vagus in The Ameliorative Effect of Nicotine on Rat Pancreatitis Model

Abstract

The anti-inflammatory effects of nicotine on various organs and the involvement of the cholinergic anti-inflammatory pathway activation in these effects were previously shown. The purpose of the study is to evaluate the effects of nicotine treatment on acute pancreatitis model and to investigate the association of cholinergic pathway with these effects.In Wistar albino rats common pancreatic-biliaryduct was ligated (PBDL) or sham-operation were performed. In PBDL rats, vagal afferent denervation (perivagal capsaicin; 10mg/ml) or truncal vagotomy was appliedor the vagi were left intact. Postoperatively, PBDL groups were treated intraperitoneally with either nicotine (1 mg/kg/day) or saline for 4 days. Following decapitation on the 4th day, serum tumor necrosis factor (TNF)-and interleukin (IL)-10 levels andmyeloperoxidase activity (MPO), malondialdehiyde (MDA) and glutathione (GSH) levels in the pancreas, liver and lung tissues were measured, and histopathological analyses were made. While the TNF-α level was significantly higher in the PBDL group than in the YC group, it was significantly lower in the subjects takennicotine treatment. IL-10 level was lower in the PBDL group treated with saline than in the YC group and an increase was observed with nicotine treatment.Compared to sham-operated group, microscopic damage scores, MDA, MPO levels in the pancreas, liver and lung tissues of the PBDL group were elevated, while GSH levels were reduced. Nicotine treatment depressed MDA and MPO levels,preserved GSH contents and reduced histological damage in the pancreas and liver. Vagal afferent denervation or truncal vagotomy did not alter these protective effects of nicotine. The results suggest that the protective effects of nicotine on pancreatic inflammation could occur independently of the vagal pathways, but directly by the activation of 7nAChR on the immune cells and by inhibiting neutrophil infiltration and pro-inflammatory cytokine release.

Keywords

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Details

Primary Language

Turkish

Subjects

Internal Diseases

Journal Section

Research Article

Authors

Meltem Kolgazi ^*
Türkiye

Zozan Güleken
Türkiye

Bircan Kolbaşı
Türkiye

Canberk Sami Başıbüyük
Türkiye

Feriha Ercan
Türkiye

Berrak Yeğen
Türkiye

Publication Date

April 1, 2021

Submission Date

February 11, 2020

Acceptance Date

-

Published in Issue

Year 2021 Volume: 12 Number: 2

DOI

https://doi.org/10.31067/acusaglik.849995

IZ

https://izlik.org/JA58DE78BP

Cite

RIS / Bibtex

EndNote

Kolgazi M, Güleken Z, Kolbaşı B, Başıbüyük CS, Ercan F, Yeğen B (April 1, 2021) Nikotinin Sıçan Pankreatit Modelindeki Hafifletici Etkisinde Vagusun Rolünün Araştırılması. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi 12 2 166–175.