GNAI2 Geninde İki Aday Polimorfizm

Emrah Kara; Cevdet Nacar; Beki Kan

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GNAI2 Geninde İki Aday Polimorfizm

Öz

Amaç: GNAI2 geninin 193 numaralı kodonunda bir hipofiz tümöründe ilk kez saptanmış olan bir mutasyonun polimorfizm olma olasılığının incelenmesi. Hastalar ve Yöntem: Kandan elde edilen DNA’lar GNAI2 geninin beşinci ve altıncı eksonları ile aralarındaki intron bölgesini içerecek şekilde Polimeraz Zincir Tepkimesi PZT ile çoğaltıldı. PZT ürünleri Tek Sarmal Konformasyon Polimorfizmi SSCP yöntemiyle mutasyon belirleme MDE ve poliakrilamit jellerinde incelendi. Seçilen bazı örneklerin otomatik DNA analizi gerçekleştirildi. Bulgular: SSCP analizinde farklı örnekçe belirlenen iki örneğin otomatik DNA dizi analizlerinde, 193 numaralı kodonda mutasyon olmadığı ancak GNAI2 geninin beşinci ve altincı eksonları arasındaki intron bölgesinde bir C/T C20151 değişikliğinin olduğu saptandı. Sonuç: Bulgularımız GNAI2 geninin 193 numaralı kodonunda daha önce belirlenen değişikliğin polimorfizm olma olasılığını desteklememektedir.

Anahtar Kelimeler

G proteini,polimorfizm

References

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Turkish

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Emrah Kara

Cevdet Nacar

Beki Kan

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June 1, 2012

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Year 1970 Number: 2

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https://izlik.org/JA56PD99JE

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Kara E, Nacar C, Kan B (June 1, 2012) GNAI2 Geninde İki Aday Polimorfizm. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi 2 104–107.

Two Candidate Polymorphisms of The Gnai2 Gene

GNAI2 Geninde İki Aday Polimorfizm

Öz

Anahtar Kelimeler

Two Candidate Polymorphisms of The Gnai2 Gene

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