Prenatal Dönemde 900Mhz Elektromanyetik Alanın Sıçan Omurilik Dokusu Üzerine Etkilerinin Histopatolojik Olarak İncelenmesi

Tolga Mercantepe; Levent Tümkaya; Mehmet Fatih Gökçe; Barış Uzunok; Zehra Suzan Topal; Seda Çınar; Erva Esmer

EN TR

The Effect of 900Mhz Electromagnetic Field On Rat Spinal Cord In The Prenatal Period: a Histopathological Investigation

Abstract

Objective: Technological devices, which are introduced into our daily lives and radiate increasingly more electromagnetic waves with a certain frequency, expose people to an electromagnetic field. It is also well known that electromagnetic fields caused by these devices have certain adverse effects on the embryonic and pubertal development, and cause pathological changes in the tissues. Pathological changes, which emerge as a result of the damages in the spinal tissue, may cause signal conduction problems in the brain, and peripheral nervous system. Therefore, in our study, we investigated with histopathological analysis techniques the changes in the spinal tissue of rats during the prenatal period that were exposed to an electromagnetic field frequency: 900MHz .Materials and Methods: Total 18 rats 6 pregnant Spraque-Dawley female rats and 12 baby rats delivered by these rats were included into our study. We divided the rats into two groups as electromagnetic field group and control group. The electromagnetic group consisted of 6 newborn rats, which were exposed to the electromagnetic field during the prenatal period. Pregnant rats in the electromagnetic field group were exposed to a magnetic field of 900MHz radio frequency with a digital signal generator ER device that was placed in the center of the cage for 24 hours and 20 days. Newborn rats were kept 60 days for the pubertal stage development, and then they were anesthetized.Results: We observed that the neuronal perikaryon and oligodendrocytes in the samples of the electromagnetic field group had pyknotic nuclear structures. We also determined that the Kaspaz-3 positive cells were increased in the neurons and oligodendrocytes in the gray matter of the spinal tissue samples in the electromagnetic field group compared to the control group p=0.0001, p

Keywords

Spinal cord,electromagnetic field,rat

Prenatal Dönemde 900Mhz Elektromanyetik Alanın Sıçan Omurilik Dokusu Üzerine Etkilerinin Histopatolojik Olarak İncelenmesi

Öz

Amaç: Günümüzde yaşantımıza giren ve giderek artan belli bir frekansta elektromanyetik dalga yayılımına neden olan teknolojik cihazlar insanları elektromanyetik alana maruz bırakmaktadır. Bu cihazlar tarafından yayılan elektromanyetik alanın embriyonal ve pubertal gelişim süresince olumsuz etkilere neden olduğu ve dokularda patolojik değişikliklere sebep olduğu bilinmektedir. Omurilik, dokusunda gelişebilecek hasar sonucunda ortaya çıkabilecek patolojik değişiklikler beyin ve periferik sinir sistemi arasındaki sinyal iletiminde sorunlara neden olabilir. Bu nedenle çalışmamızda prenatal dönemde histopatolojik analiz teknikleriyle 900MHz frekansında elektromanyetik alana maruz bırakılan sıçanların omurilik dokusunda meydana gelen değişiklikleri araştırılmıştır.Gereç ve Yöntem: Çalışmamızda 6 adet gebe Sprague-Dawley dişi sıçan ve bu gebe sıçanlardan elde edilen 12 adet yavrularından olmak üzere toplam 18 adet sıçan kullanıldı. Çalışmamız kontrol ve elektromanyetik alan olmak üzere iki gruba ayrıldı. Elektromanyetik alan grubu, doğum öncesi dönemde elektromanyetik alana maruz bırakılmış altı yeni doğmuş sıçandan oluşmaktadır. Elektromanyetik alan grubuna ait gebe sıçanların bulunduğu kafeslerin ortasına 20 gün süreyle 24 saat boyunca dijital sinyal üreteci ER cihazı ile 900MHz radyo frekansı ile manyetik alana maruz bırakıldı. Yeni doğan sıçanlar pubertal evre gelişimi için 60 gün bekletildikten sonra uyutulmuştur.Bulgular: Elektromanyetik alan grubuna ait örnekler incelendiğinde özelikle nöronların perikaryonları ve oligodendrositlerin piknotik çekirdek yapılarına sahip olduklarını gözlemledik. Elektromanyetik alan grubu omurilik doku örneklerinin gri cevherlerindeki nöronlarda ve oligodendrositlerde Kaspaz-3 pozitif hücre sayısı kontrol grubuna kıyasla anlamlı olarak artmış olduğu saptanmıştır p=0,001, p

Anahtar Kelimeler

Omurilik,elektromanyetik alan,sıçan

References

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Details

Primary Language

Turkish

Subjects

-

Journal Section

-

Authors

Tolga Mercantepe

Levent Tümkaya

Mehmet Fatih Gökçe

Barış Uzunok

Zehra Suzan Topal

Seda Çınar

Erva Esmer

Publication Date

June 1, 2018

Submission Date

-

Acceptance Date

-

Published in Issue

Year 2018 Number: 2

IZ

https://izlik.org/JA94JM63FH

Cite

RIS / Bibtex

EndNote

Mercantepe T, Tümkaya L, Gökçe MF, Uzunok B, Topal ZS, Çınar S, Esmer E (June 1, 2018) Prenatal Dönemde 900Mhz Elektromanyetik Alanın Sıçan Omurilik Dokusu Üzerine Etkilerinin Histopatolojik Olarak İncelenmesi. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi 2 137–141.