Evaluation of Feed Strategy for High Quality Biosimilar IgG Production in CHO Cell Fed-batch Process

Duygu Ayyıldız Tamis; Berna Ustuner; Secil Dayankac Unver; Tunç Turgut; Deniz Baycın

doi:10.31067/acusaglik.934263

EN

Evaluation of Feed Strategy for High Quality Biosimilar IgG Production in CHO Cell Fed-batch Process

Abstract

Purpose: Chinese Hamster Ovary (CHO) cells are currently the leading hosts for biosimilar Immunoglobulin G (IgG) production in the biopharmaceutical industry. Most eukaryotic proteins are glycosylated, and charge variants affect both the in vivo and in vitro properties of monoclonal antibodies (mAb). Adjusting the N-glycosylation patterns and charge variants while achieving high antibody titer is a production challenge. In this study, the effects of feed type and strategy on cell growth, product titer, glycosylation and charge variation were investigated using different CHO clones producing different IgG mAbs. Methods: Cultivated CHO cells were supplemented with different feeding schemes, under fed-batch productions of 14 days. Screenings were conducted in spin-tubes and further investigated in 3L bioreactor systems. Results: Change in feed strategy decreased productivities by 10.4% (P < 0.05), while it increased non-fucosylated glycoforms by 33.3% and enhanced galactosylation up to 3-folds. Basic variants were observed to increase 2.5 folds. Conclusion: These remarkable alterations are of great importance in terms of mAb quality, in a manufacturing point of view, as they provide modulation of efficacy and safety. This reveals that feed strategy is a major driving force that significantly impacts culture longevity, galactosylated glycoforms, high-mannose glycan contents and charge variants.

Keywords

References

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Details

Primary Language

English

Subjects

Microbiology

Journal Section

Research Article

Authors

Duygu Ayyıldız Tamis ^*
0000-0002-3073-2857
Türkiye

Berna Ustuner
0000-0002-4939-3103
Türkiye

Secil Dayankac Unver
0000-0003-2258-4898
Türkiye

Tunç Turgut
0000-0002-8512-0321
Türkiye

Deniz Baycın
0000-0001-9577-4495
Türkiye

Publication Date

January 1, 2022

Submission Date

May 7, 2021

Acceptance Date

November 10, 2021

Published in Issue

Year 2022 Volume: 13 Number: 1

DOI

https://doi.org/10.31067/acusaglik.934263

IZ

https://izlik.org/JA67UY39JY

Cite

RIS / Bibtex

EndNote

Ayyıldız Tamis D, Ustuner B, Dayankac Unver S, Turgut T, Baycın D (January 1, 2022) Evaluation of Feed Strategy for High Quality Biosimilar IgG Production in CHO Cell Fed-batch Process. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi 13 1 6–15.

Öz

Evaluation of Feed Strategy for High Quality Biosimilar IgG Production in CHO Cell Fed-batch Process

Abstract

Keywords

References

Details

Primary Language

Subjects

Journal Section

Authors

Publication Date

Submission Date

Acceptance Date

Published in Issue

DOI

IZ

Cite