Research Article

Detection of H. pylori in Pediatric Patients’ Stool Sample by Multiplex Urease PCR

Volume: 13 Number: 1 January 1, 2022
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Detection of H. pylori in Pediatric Patients’ Stool Sample by Multiplex Urease PCR

Abstract

Background and objectives: This study aims to detect the H. pylori infection in pediatric patients’ stool samples by using a multiplex urease PCR assay. Materials and Methods: A retrospective analysis was performed and the H. pylori multiplex urease PCR test from a stool sample of 55 pediatric patients was evaluated from August 2017 to November 2018 compared with the H. pylori antigen test and histopathology. Results: Thirty-six patients (65%) were detected H. pylori-positive including nineteen boys (50%) and nineteen girls (50 %) by H. pylori multiplex urease PCR test. Fifteen (54 %) of the positive patients were in the 0-6 age range, eighteen (95%) in the 7-12 age range, and three (38%) in the 13-18 age range. Comparison results with the histopathology and H. pylori antigen test were showed that the positive predictive value of the multiplex urease PCR test for the stool sample was 72.22%. The test could detect a negative sample of 100 %. Conclusions: Due to the results of this study it was showed that the prevalence of H. pylori infection is still high for pediatric patients. The multiplex urease PCR test for stool samples could be used in the clinic detection of H. pylori as a non-invasive method and easy to an applicable method, but it needs to be evaluated for high specificity and sensitivity to detect accurate results.

Keywords

References

  1. Reference 1. Stewart BW, Wild CP.,World cancer report 2014. Chapter 5.4: Stomach cancer. Lyon, France: International Agency for Research on Cancer
  2. Reference 2. Frenck RW Jr., Clemens J. Helicobacter in the developing world. Microbes and infection. Institut Pasteur. 2003;5(8):705–13. Epub 2003/06/20. pmid:12814771.
  3. Reference 3. de Brito BB, da Silva FAF, Soares AS, et al. Pathogenesis and clinical management of Helicobacter pylori gastric infection. World J Gastroenterol. 2019;25(37):5578-5589. doi:10.3748/wjg.v25.i37.5578
  4. Reference 4. Fischbach W, Malfertheiner P. Helicobacter Pylori Infection. Dtsch Arztebl Int. 2018;115(25):429-436. doi:10.3238/arztebl.2018.0429
  5. Reference 5. Can F, Karahan C, Dolapci I, Demirbilek M, Tekeli A, Arslan H. Urease activity and urea gene sequencing of coccoid forms of H. pylori induced by different factors. Curr Microbiol. 2008;56(2):150-155. doi:10.1007/s00284-007-9047-y.
  6. Reference 6. Okullu SO, Kocagöz T, Tiftikçi A, Yazgan AS, Tozun N, et al. (2017) A Multiplex-Urease PCR Assay for Detection of Helicobacter pylori Infection Directly from Gastric Biopsy Specimens and Comparison of Multiplex-Urease PCR Results with Rapid Urease Test and Histopathology. Clin Microbiol 6: 279. doi:10.4172/2327-5073.1000279
  7. Reference 7. https://www.medcalc.org/calc/diagnostic_test.php

Details

Primary Language

English

Subjects

Medical Microbiology

Journal Section

Research Article

Authors

Publication Date

January 1, 2022

Submission Date

May 18, 2021

Acceptance Date

September 10, 2021

Published in Issue

Year 2022 Volume: 13 Number: 1

DOI

https://doi.org/10.31067/acusaglik.938789

IZ

https://izlik.org/JA56DC38XR

Cite

RIS / Bibtex
EndNote
Öktem Okullu S, Işık OC, Kocagöz S, Akyar I (January 1, 2022) Detection of H. pylori in Pediatric Patients’ Stool Sample by Multiplex Urease PCR. Acıbadem Üniversitesi Sağlık Bilimleri Dergisi 13 1 16–20.

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