Research Article
Year 2023,
Volume: 5 Issue: 1, 22 - 33, 04.05.2023
Abstract
References
- Acet T, Ozcan K, Zengin G. An assessment of phenolic profiles, fatty acid compositions, and biological activities of two Helichrysum species: H. plicatum and H. chionophilum. J Food Biochem 2019:1–11.
- Apaydin Yildirim B, Kordali S, Terim Kapakin KA, Yildirim F, Aktas Senocak E, Altun S. Effect of Helichrysum plicatum DC. subsp plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats. J Zhejiang Univ Sci B 2017;18:501–11.
- Appendino, G., Ottino, M., Marquez, N., Bianchi, F., Giana, A., Ballero, M., Sterner, O., Fiebich, B.L., Munoz, E. (2007). Arzanol, an Anti-inflammatory and Anti-HIV-1Phloroglucinolα-Pyrone from Helichrysum italicum ssp. Microphyllum, Natural Products (70) 4, 608-612.
- Azwanida, NN. (2015). A. review on the extraction methods use in medicinal plants, principle, strength and limitation. Med. Aromat. Plants, 4(3): 6.
- Bigovic, D., Brankovic, S., Kitic, D., Radenkovic, M., Jankovic, T., Savikin, K., Zivanovic,S.(2010). Relaxant effect of the ethanol extract of Helichrysum plicatum (Asteraceae) on isolated ratileum contractions. Molecules, 15(5): 3391-3401.
- Chiavari-Fredorigo, M.O., Barbosa, L.N., dos Sentos, I.C., da Silva, G.R., de Castro, A.F. (2020). Antimicrobial activity of Asteraceae species against bacterial pathogens isolated from postmenopausal women. Plos one, 1-14.
- Clinical and Laboratory Standards Institute (CLSI) (2016). Performance Standards for Antimicrobial Susceptibility Testing; Twentieth Informational Supplement. United States: Wayne Press.
- Demir A, Taban BM, Aslan M, Yeşilada E, Aytaç SA. Antimicrobial effect of Helichrysum plicatum subsp. plicatum. Pharma Biol. 2009;47(4): 289-297.
- Davis, PH., Mill, RR., Tan, K. (1988). Flora of Turkey and East Aeagen Islands. v. X. Edinburgh: Edinburgh University Press, p. 159-160.
- Erik, S., Güner A., Özhatay N., Ekim T., Başer KHC. (2000) Helichrysum gaertner. Flora of Turkey and East Aeagen Islands, 11: 153-154.
- Eroglu, E.H., Budak, U., Hamzaoglu, E., Aksoy, A., Albayrak, S. (2010). In Vıtro Cytotoxıc Effects Of Methanol Extracts Of Sıx Helıchrysum Taxa Used In Tradıtıonal Medıcıne, Pak. J. Bot., 42(5): 3229-3237.
- Galovic, A.J., Lijescovic, N.J., Vidovic, S. Vladic, J., Mrkonic, Z., Gigov, S., Ilıc, M., Kojic, V., Jakimov, D., Zloh, M. (2022). Potential of Helicrysum italicum cultivated in urban environment: SCCO2 extract cytotoxicity & NF-kB activation in HeLa, MCF-7 and MRC-5 cells. Sustainable Chemistry and Pharmacy 26, 100622.
- Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M.T. (edlr.), (2012). Türkiye bitkileri listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. İstanbul.
- Gunes A, Kordali S, Turan M, Bozhuyuk AU. Determination of antioxidant enzyme activity and phenolic contents of some species of the Asteraceae family from medicanal plants. Ind Crop Prod 2019;137:208–13.
- Kaya, E. (2022). Helichrysum plicatum Çiçeklerinin Su, Etanol, Aseton, Kloroform ve Hekzan Ekstrelerinin Antioksidan Aktiv. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 5(2), 840–849.
- Kordali, S., Çakır, A., Akçin, T.A., Mete, E., Akçin, A., Aydın, T., Kılıç, H. (2009). Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola Hub-Mor. and Achillea biebersteinii Afan. (Asteraceae). Industrial crops and products 29 (2009) 562–570.
- Kothavade, P.S., Nagmoti, D.M., Bulani, V.D., Juvekar, A.R. (2013). Arzanol, a Potent mPGES-1 Inhibitor: Novel Anti-Inflammatory Agent, The Scientific World Journal.
- Kulevanova, S., Stefova, M., Stafiloy, T. (2000). HPLC idebtification and determination of flavone aglycones in Helichrysum plicatum DC. (Asteraceae). Pharmazie 2000;55:391–2.
- Mosmann, T. (1983). Rapid Colorometric Assay for Cellular Growth and Survival: Aplication to Proliferation and Cytotoxicity Assay and Cell Biology. Journal of Immünological Methods, 65(2), 55-63.
- Petropoulos, S.A., Fernandes, A., Tzortzkis, N., Sokovic, M., Ciric, A., Barros, L., Ferreira, I.C.F.R. (2019). Bioactive compounds content and antimicrobial activities of wild edible Asteraceae species of the Mediterranean flora under commercial cultivation conditions, Food Research International 119, 859-868.
- Poudineh, F., Azari, A.A., Fozouni, l. (2021). Antibacterial Activity of Ethanolic Extract of Matricaria chamomilla, Malva cylvestris, and Capsella bursa-pastoris against MultidrugResistant Pseudomonas aeruginosa Strains. Avicenna Journal of Clinical Microbiology and Infection. 8(1), 23-26.
- Rosa, A., Atzeri, A., Nieddu, M., Appendino, G. (2017). New insights into the antioxidant activity and cytotoxicity of arzanol and effect of methylation on its biological properties, Chemistry and Physics of Lipids 55-64.
- Sala, A., Carmen Recio, M., Giner, R.M., Manez, S., Tournier, H., Schinella, G., Rios, J.L. (2002). Antiinflammatory and antioxidant properties of Helichrysum italicum, Journal of Pharmacy and Pharmacology 54(3), 365-371.
- Taşkın, T., Gezmiş, T., Çam, M.E., Taşkın, D., Çelik, B.Ö., Şenkardeş, İ., Selçuk, S.S. (2020). The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. Plicatum. Brazilian Journal of Pharmaceutical Sciences. 56, 1-10.
- Tatlı, I.I., Sahpaz, S., Akkol, E.K., Martin-Nizard, F., Gressier B, Ezer N, et al. Antioxidant, antiinflammatory, and antinociceptive activities of Turkish medicinal plants. Pharm Biol 2009;47:916–21.
- Tekin, Z., Küçükbay, Z.F., Dikme, A. (2021). In Vitro Antioxidant Activities of Methanol Extracts of Three Achillea Species from Turkey. Journal of the Turkish Chemical Society Section A: Chemistry. 8(2), 483-490.
IN VITRO STUDY ON ANTIMICROBIAL AND CYTOTOXIC ACTIVITY OF METHANOL EXTRACT of Helichrysum plicatum subsp. polyphyllum (Asteraceae)
Abstract
Helichrysum plicatum subsp. polyphyllum (H. plicatum), is an important species belonging to the family Acteraceae, which is used in alternative medicine. For this reason, it was aimed to determine the antimicrobial and cytotoxic activity of H. plicatum extract in this study. Firstly, H. plicatum was extracted with methanol for 6 hours using a Soxhlet. Secondly, the antimicrobial activity of the extract was tested against Gram-positive (Staphylococcus aureus ATCC®25923, Bacillus cereus ATCC®7064, Bacillus subtilis ATCC®6633) and Gram-negative (Escherichia coli ATCC®25922, Klebsiella pneumoniae ATCC®706003, Salmonella Enteritidis ATCC®13076) bacteria and a yeast (Candida albicans ATCC®10231) using well diffusion and microdilution methods. Finally, the cytotoxic activity of H. plicatum extract on DLD-1 (human colon adenocarcinoma) and CCD-18Co (normal colon) cell lines was determined by MTT method. According to the results of the antimicrobial effect, H. plicatum extract was found to have a stronger antibacterial activity against Salmonella Enteritidis (24.13±1.15 and 156µg/mL) among Gram-negative bacteria. It was determined to have inhibitory activity against also Bacillus cereus (16.66±1.52 and 312µg/mL) among Gram-positive bacteria. In addition, the extract showed a strong antifungal effect on Candida albicans (22.66±0.57 and 312µg/mL). In addition, the H. plicatum extract did not show any cytotoxic effect on CCD-18Co cells, while the IC50 value on the DLD-1 cell line was determined as 8.17mg/mL. This indicates that the plant extract has a selective toxic effect on cancer cells. These results reveal that H. plicatum has the potential to be used for therapeutic purposes after being supported by further studies.
References
- Acet T, Ozcan K, Zengin G. An assessment of phenolic profiles, fatty acid compositions, and biological activities of two Helichrysum species: H. plicatum and H. chionophilum. J Food Biochem 2019:1–11.
- Apaydin Yildirim B, Kordali S, Terim Kapakin KA, Yildirim F, Aktas Senocak E, Altun S. Effect of Helichrysum plicatum DC. subsp plicatum ethanol extract on gentamicin-induced nephrotoxicity in rats. J Zhejiang Univ Sci B 2017;18:501–11.
- Appendino, G., Ottino, M., Marquez, N., Bianchi, F., Giana, A., Ballero, M., Sterner, O., Fiebich, B.L., Munoz, E. (2007). Arzanol, an Anti-inflammatory and Anti-HIV-1Phloroglucinolα-Pyrone from Helichrysum italicum ssp. Microphyllum, Natural Products (70) 4, 608-612.
- Azwanida, NN. (2015). A. review on the extraction methods use in medicinal plants, principle, strength and limitation. Med. Aromat. Plants, 4(3): 6.
- Bigovic, D., Brankovic, S., Kitic, D., Radenkovic, M., Jankovic, T., Savikin, K., Zivanovic,S.(2010). Relaxant effect of the ethanol extract of Helichrysum plicatum (Asteraceae) on isolated ratileum contractions. Molecules, 15(5): 3391-3401.
- Chiavari-Fredorigo, M.O., Barbosa, L.N., dos Sentos, I.C., da Silva, G.R., de Castro, A.F. (2020). Antimicrobial activity of Asteraceae species against bacterial pathogens isolated from postmenopausal women. Plos one, 1-14.
- Clinical and Laboratory Standards Institute (CLSI) (2016). Performance Standards for Antimicrobial Susceptibility Testing; Twentieth Informational Supplement. United States: Wayne Press.
- Demir A, Taban BM, Aslan M, Yeşilada E, Aytaç SA. Antimicrobial effect of Helichrysum plicatum subsp. plicatum. Pharma Biol. 2009;47(4): 289-297.
- Davis, PH., Mill, RR., Tan, K. (1988). Flora of Turkey and East Aeagen Islands. v. X. Edinburgh: Edinburgh University Press, p. 159-160.
- Erik, S., Güner A., Özhatay N., Ekim T., Başer KHC. (2000) Helichrysum gaertner. Flora of Turkey and East Aeagen Islands, 11: 153-154.
- Eroglu, E.H., Budak, U., Hamzaoglu, E., Aksoy, A., Albayrak, S. (2010). In Vıtro Cytotoxıc Effects Of Methanol Extracts Of Sıx Helıchrysum Taxa Used In Tradıtıonal Medıcıne, Pak. J. Bot., 42(5): 3229-3237.
- Galovic, A.J., Lijescovic, N.J., Vidovic, S. Vladic, J., Mrkonic, Z., Gigov, S., Ilıc, M., Kojic, V., Jakimov, D., Zloh, M. (2022). Potential of Helicrysum italicum cultivated in urban environment: SCCO2 extract cytotoxicity & NF-kB activation in HeLa, MCF-7 and MRC-5 cells. Sustainable Chemistry and Pharmacy 26, 100622.
- Güner, A., Aslan, S., Ekim, T., Vural, M., Babaç, M.T. (edlr.), (2012). Türkiye bitkileri listesi (Damarlı Bitkiler). Nezahat Gökyiğit Botanik Bahçesi ve Flora Araştırmaları Derneği Yayını. İstanbul.
- Gunes A, Kordali S, Turan M, Bozhuyuk AU. Determination of antioxidant enzyme activity and phenolic contents of some species of the Asteraceae family from medicanal plants. Ind Crop Prod 2019;137:208–13.
- Kaya, E. (2022). Helichrysum plicatum Çiçeklerinin Su, Etanol, Aseton, Kloroform ve Hekzan Ekstrelerinin Antioksidan Aktiv. Osmaniye Korkut Ata Üniversitesi Fen Bilimleri Enstitüsü Dergisi, 5(2), 840–849.
- Kordali, S., Çakır, A., Akçin, T.A., Mete, E., Akçin, A., Aydın, T., Kılıç, H. (2009). Antifungal and herbicidal properties of essential oils and n-hexane extracts of Achillea gypsicola Hub-Mor. and Achillea biebersteinii Afan. (Asteraceae). Industrial crops and products 29 (2009) 562–570.
- Kothavade, P.S., Nagmoti, D.M., Bulani, V.D., Juvekar, A.R. (2013). Arzanol, a Potent mPGES-1 Inhibitor: Novel Anti-Inflammatory Agent, The Scientific World Journal.
- Kulevanova, S., Stefova, M., Stafiloy, T. (2000). HPLC idebtification and determination of flavone aglycones in Helichrysum plicatum DC. (Asteraceae). Pharmazie 2000;55:391–2.
- Mosmann, T. (1983). Rapid Colorometric Assay for Cellular Growth and Survival: Aplication to Proliferation and Cytotoxicity Assay and Cell Biology. Journal of Immünological Methods, 65(2), 55-63.
- Petropoulos, S.A., Fernandes, A., Tzortzkis, N., Sokovic, M., Ciric, A., Barros, L., Ferreira, I.C.F.R. (2019). Bioactive compounds content and antimicrobial activities of wild edible Asteraceae species of the Mediterranean flora under commercial cultivation conditions, Food Research International 119, 859-868.
- Poudineh, F., Azari, A.A., Fozouni, l. (2021). Antibacterial Activity of Ethanolic Extract of Matricaria chamomilla, Malva cylvestris, and Capsella bursa-pastoris against MultidrugResistant Pseudomonas aeruginosa Strains. Avicenna Journal of Clinical Microbiology and Infection. 8(1), 23-26.
- Rosa, A., Atzeri, A., Nieddu, M., Appendino, G. (2017). New insights into the antioxidant activity and cytotoxicity of arzanol and effect of methylation on its biological properties, Chemistry and Physics of Lipids 55-64.
- Sala, A., Carmen Recio, M., Giner, R.M., Manez, S., Tournier, H., Schinella, G., Rios, J.L. (2002). Antiinflammatory and antioxidant properties of Helichrysum italicum, Journal of Pharmacy and Pharmacology 54(3), 365-371.
- Taşkın, T., Gezmiş, T., Çam, M.E., Taşkın, D., Çelik, B.Ö., Şenkardeş, İ., Selçuk, S.S. (2020). The in vitro and in vivo investigation of biological activities and phenolic analysis of Helichrysum plicatum subsp. Plicatum. Brazilian Journal of Pharmaceutical Sciences. 56, 1-10.
- Tatlı, I.I., Sahpaz, S., Akkol, E.K., Martin-Nizard, F., Gressier B, Ezer N, et al. Antioxidant, antiinflammatory, and antinociceptive activities of Turkish medicinal plants. Pharm Biol 2009;47:916–21.
- Tekin, Z., Küçükbay, Z.F., Dikme, A. (2021). In Vitro Antioxidant Activities of Methanol Extracts of Three Achillea Species from Turkey. Journal of the Turkish Chemical Society Section A: Chemistry. 8(2), 483-490.
There are 26 citations in total.
Details
| Primary Language | English |
|---|---|
| Subjects | Clinical Sciences |
| Journal Section | Research Articles |
| Authors | |
| Publication Date | May 4, 2023 |
| Submission Date | December 17, 2022 |
| Published in Issue | Year 2023 Volume: 5 Issue: 1 |